The objective of this study was to evaluate the applicability of the Petrifilm™ plates to enumerate microbial groups in sheep milk. Samples of sheep milk (n = 30) were plated simultaneously, to enumerate mesophilic aerobes, total coliforms, lactic acid bacteria, Staphylococcus aureus and Escherichia coli, using convencional reference protocols and Petrifilm™ plates. The results were compared using McNemar's test, linear regression and ANDVA (p < 0,05). The results demonstrated good significant between conventional methodologies and Petrifilm™ plates. Further, the Petrifim™ STX for counting S. aureus had higher recoverability of bacteria compared with the conventional methodology. Based on the results obtained and in view of the ease and rapidity procedures results, Petrifim ™ plates may be considered as alternatives for microbiological testing in sheep milk.Keywords: milk analyzes; sheep's milk; Escherichia coli; Aerobic mesophilic bacteria.Practical Application: This study generated knowledge for the practical application of the Petrifilm™ system in microorganisms search in sheep milk. From this study was possible to conclude that the use of this system is reliable for microorganisms surveyed, facilitating the work, and generate faster results, an important factor when it comes to monitoring of food-borne outbreaks. Thus, it was possible to verify the adequacy of Petrifilm™ system as a new alternative for enumeration of mesophilic aerobic microorganisms, coliforms, lactic acid bacteria, Staphylococcus aureus and Escherichia coli. Finally, the use of Petrifilm™ system generated reliable results more easily and more quickly, including facilitating the development of the work in the laboratory microbiological analyzes of food, because of no necessity of using large quantities of materials, glassware, petri plates, pipettes, while minimizing the preparation of material for the analyzes.
We analyzed the flow-volume curves of 50 patients with complaints of snoring and daytime sleepiness in treatment at the Pneumology Unit of the University Hospital of Brasília. The total group was divided into snorers without obstructive sleep apnea (OSA) (N = 19) and snorers with OSA (N = 31); the patients with OSA were subdivided into two groups according to the apnea/hypopnea index (AHI): AHI<20/h (N = 14) and AHI>20/h (N = 17). The control group (N = 10) consisted of nonsmoking subjects without complaints of snoring, daytime sleepiness or pulmonary diseases. The population studied (control and patients) consisted of males of similar age, height and body mass index (BMI); spirometric data were also similar in the four groups. There was no significative difference in the ratio of forced expiratory and inspiratory flows (FEF 50% /FIF 50% ) in any group: control, 0.89; snorers, 1.11; snorers with OSA (AHI<20/h), 1.42, and snorers with OSA (AHI>20/h), 1.64. The FIF at 50% of vital capacity (FIF 50% ) of snoring patients with or without OSA was lower than the FIF 50% of the control group (P<0.05): snorers 4.30 l/s; snorers with OSA (AHI<20/h) 3.69 l/s; snorers with OSA (AHI>20/h) 3.17 l/s and control group 5.48 l/s. The FIF 50% of patients with severe OSA (AHI>20/h) was lower than the FIF 50% of snorers without OSA (P<0.05): 3.17 l/s and 4.30 l/s, respectively. We conclude that 1) the FEF 50% /FIF 50% ratio is not useful for predicting OSA, and 2) FIF 50% is decreased in snoring patients with and without OSA, suggesting that these patients have increased upper airway resistance (UAR).
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