Gas Chromatography-Mass Spectrometry Analysis and Antimicrobial and Antioxidant Activities of Some Orchid (Orchidaceae) Species Growing in Turkey

Ertürk, Ömer; Ayvaz, Melek Çöl; Çil, Elif; Bağdatlı, Emine

doi:10.1590/1678-4324-2023210265

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Once we had characterized the volatile portion of O. purpurea , we focused our attention on the characterization of the non-volatile constituents. Thus, to the best of our knowledge, only the non-volatile fraction obtained by the hypogeal part of O. purpurea has already been analyzed in previous work [ 23 ]. Consistently, both leaves and flowers were sequentially extracted by exploiting a Soxhlet apparatus with n -hexane, ethyl acetate, and methanol.…”

Section: Resultsmentioning

confidence: 99%

Qualitative Metabolite Profiling of Orchis purpurea Huds. by GC and UHPLC/MS Approaches

Cavalloro,

Pagliari,

Gosetti

et al. 2024

Plants

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Orchids are experiencing wide success in ornamental, medicinal, and food fields. The reason for their success is correlated with both their morphology and metabolomics, the latter linked to their taste and biological effects. Despite many orchids having already been the subject of chemotaxonomic works, some of them are still untapped, like the case of Orchis purpurea. O. purpurea is one of the most common species of the genus Orchis, present in hedgerows, verges, and light woodland, where it is one of the few herbaceous plants able to be unpleasant to herbivorous animals. Essential oil from roots, stems, leaves, and flowers were analyzed via GC/MS analyses, revealing the presence of 70 compounds, with a clear prevalence of coumarin. The high concentration of this metabolite may explain the resistance of O. purpurea to herbivores, being associated with appetite-suppressing properties and a bitter taste. Non-volatile fractions were analyzed via UHPLC-MS analysis revealing the presence of hydroxycinnamic acid derivatives, polyphenols, and glycosidic compounds, probably responsible for their color and fragrance. Taken together, the herein presented results shed light on both the defensive strategy and the chemotaxonomy of O. purpurea.

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Section: Resultsmentioning

confidence: 99%

Qualitative Metabolite Profiling of Orchis purpurea Huds. by GC and UHPLC/MS Approaches

Cavalloro,

Pagliari,

Gosetti

et al. 2024

Plants

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Phenolic and Antioxidant Profile: Ftir and Lc-MS Analyses of Serapias Orientalis

Aytar,

Özdener Kömpe

2024

Ankara Ecz. Fak. Derg.

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Objective: The objective of this study is to characterize the aerial parts of S. orientalis using Fourier Transform Infrared (FTIR) spectroscopy, evaluate the phenolic content and antioxidant activity of seeds, stems, and flowers, and conduct quantitative analysis of phenolic compounds using LC-MS/MS. Material and Method: Fourier Transform Infrared (FTIR) spectroscopy was employed to characterize the aerial parts of S. orientalis. The analysis focused on identifying various functional groups such as -OH vibrations associated with polysaccharides, C-H vibrations from lipids and lignin compounds, and C=O vibrations related to cellulose derivatives. The total phenolic, flavonoid, flavanol, tannin, and proanthocyanidin contents of S. orientalis seeds, stems, and flowers were evaluated using standard analytical methods. DPPH radical scavenging activity was determined to assess antioxidant potential, with IC50 values calculated for each plant part. Quantitative analysis of phenolic compounds in the plant extract was conducted using LC-MS/MS. The abundance of various phenolic acids including P-coumaric acid, trans-ferulic acid, caffeic acid, and vanillic acid was determined. Additionally, other phenolic compounds such as gallic acid, chlorogenic acid, salicylic acid, (+) taxifolin, rutin hydrate, ellagic acid, quercetin dihydrate, and apigenin were also detected and quantified. Result and Discussion: The evaluation of phenolic content showed differences among different plant parts, with flowers exhibiting the highest total phenolic and proanthocyanidin content. Seeds demonstrated superior DPPH radical scavenging activity. Quantitative analysis of phenolic compounds using LC-MS/MS highlighted the abundance of various phenolic acids and other phenolic compounds in S. orientalis. These findings underscore the potential of S. orientalis as a valuable source of natural antioxidants. Overall, the results suggest that S. orientalis possesses significant phenolic diversity and antioxidant activity, which could contribute to its potential applications in various industries, including pharmaceuticals and nutraceuticals.

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Evaluation of Anticancer Activity and Structural Analysis of Biosynthesized Silver Nanoparticles (AgNPs) from Centella Asiatica

et al. 2024

Scopus Indexed

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Introduction: The synthesis of silver nanoparticles (AgNPs) from an ethanolic extract of Centella asiatica leaf, which exhibits anticancer characteristics, was investigated in this study. Green synthesis, a non-harmful procedure for the environment, aids in the production of nanoparticles from Centella asiatica extract. Centella asiatica has long been regarded to have beneficial properties for neurological function, anti-inflammation, and wound treatment. Objective: FT-IR, GC-MS, and thin-layer chromatography were used to find the phytochemicals present in the extract, and the anti-cancer abilities of the nanoparticles were tested against the PC3 cell line. Methods: Soxhlet extraction is used to extract the bioactive, and then nanoparticles are synthesised. Following that, they are subjected to preliminary phytochemical analysis and thin-layer chromatography. These can assess the presence or absence of phytochemicals. Soon after, GCMS is done to reveal the molecular weight of the bioactive compounds using the charge-to-mass ratio. The structural peaks of different functional groups were identified using FT-IR. Under different concentrations, the silver nanoparticles were portrayed against the PC3 cell line of prostate cancer. Result: The concentration of silver nanoparticles was shown to negatively affect cell viability. The IC50 value was 11.8 µg/ml, showing that the inhibition of growth increased with increasing doses of the AgNPs. Conclusion: Our study highlights the significance of green synthesis of AgNPs from Centella asiatica extract, with bioactive compounds identified using GC-MS showing potential as anticancer agents against PC3 cells, suggesting a promising avenue for cancer therapy.

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Gas Chromatography-Mass Spectrometry Analysis and Antimicrobial and Antioxidant Activities of Some Orchid (Orchidaceae) Species Growing in Turkey

Cited by 3 publications

References 25 publications

Qualitative Metabolite Profiling of Orchis purpurea Huds. by GC and UHPLC/MS Approaches

Qualitative Metabolite Profiling of Orchis purpurea Huds. by GC and UHPLC/MS Approaches

Phenolic and Antioxidant Profile: Ftir and Lc-MS Analyses of Serapias Orientalis

Evaluation of Anticancer Activity and Structural Analysis of Biosynthesized Silver Nanoparticles (AgNPs) from Centella Asiatica

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