Amino Acid Supplementation Improves the Production of Extracellular Peptidases by Aspergillus Section Flavi and their Ionic Immobilization

Abstract

reversibly immobilized on ionic supports, DEAE-Agarose and MANAE-Agarose the derivative showed different profiles of thermal and pH stabilities. Hence, this study revealed the basic properties and biochemical characteristics that allowed the production improvement of this class of enzyme. Moreover, with known properties stabilization and immobilization process is required to further explore its biotechnological capacities.

Género Aspergillus: fuente potencial de péptidos bioactivos

“…Aspergillus flavus Peptidasa, fitasa, proteasa, lipasa, DNasa, elastasa, keratinasa, quitinasa, amilasa, poligalacturonasa [12]- [16] Aspergillus oryzae Hidrolasa, amilasa, tanasa, proteasa, fitasa, lipasa, transferasa, xylenasa, hexosaminidasa, naranginasa, peptidasa [12], [17]- [25] Aspergillus tamarii Proteasa, keratinasa, lipasa, DNasa, elastasa, xylanasa, tanasa [11], [13], [26], [27] Aspergillus nidulan Oxidasa, xylanasa [28], [29] Aspergillus clavatus Amilasa, celulasa [30] Aspergillus niger Proteasa, lipasa, peptinasa, amilasa, fitasa, keratinasa, DNasa, elastasa, OTasa, α-galactosidasa, nucleasa, hidrolasa, β-glucosidasa, xylanasa, tirosinasa [13], [17], [19], [31]- [41] Aspergillus ficuum Fitasa [19] Aspergillus fumigatus Fitasa, keratinasa, lipasa, proteasa, DNasa, elastasa, amilasa, tanasa, xylanasa [13], [19], [42]- [44] Aspergillus carbonarius Fitasa [19] Aspergillus tubingensis Fitasa, amilasa [19], [45] Aspergillus terreus Keratinasa, lipasa, proteasa, DNasa, elastasa [13] Aspergillus japonicus Fitasa [19] Aspergillus flavipes Hidrolasa, peptinasa [46], [47] Aspergillus ochraceus Tanasa [48] Aspergillus thermomutatus Invertasa [49] Aspergillus allahabadii Dextrinasa [50] Por otro lado, las nrps se caracterizan por poseer una estructura modular al menos de tres dominios: de adenilación (A), condensación (C) y proteína portadora de peptidilo (pcp), también conocida como tiolación (T) [55]. En la figura 2, se evidencia la síntesis de péptidos mediados por esta ruta metabólica.…”