Immobilization and characterization of latex cysteine peptidases on different supports and application for cow’s milk protein hydrolysis

The Use of Immobilised Enzymes for Lipid and Dairy Processing and Their Waste Products: A Review of Current Progress

“…Protease (Novo-Pro D) immobilised on agarose activated with a glyoxyl group was used to hydrolyse casein ten times without any decrease in activity [60]. Others have also reported multiple reuses of enzymes immobilised covalently [16,61,62].…”

“…Enzymes have been used for milk modifications to produce several dairy-based products such as whey protein isolate (WPI), whey protein concentrate (WPC), hydrolysed infant formula, and milk fat [9][10][11][12]. For instance, whey was obtained via enzymatic coagulation of cow milk by rennet [13] and whey protein hydrolysates were produced by the hydrolysis of cow and goat milk using different proteases including alcalase, neutrase, trypsin, bromelain, pepsin, papain, and proteinase [14][15][16]. Enzymes have also been used to produce bioactive peptides from milk proteins and dairy by-products [17,18], as well as structured and specialty lipids with functional properties via processes such as hydrolysis, esterification, and interesterification [19][20][21].…”