Resposta inflamatória peritoneal de asininos (Equus asinus) submetidos à ovariectomia por laparotomia e laparoscopia

Barros, I. O.; Nunes, T.; Spagnolo, J.D.; Santos, Jadson Pinheiro; Neto, Argemiro Soares de Aliança; Oliveira, Maria Gláucia Carlos de; Nogueira, Andressa Francisca Silva; Sousa, Rejane Santos; Silva, L.C.L.C.; Júnior, Raimundo Alves Barrêto

doi:10.1590/1678-4162-9542

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Nogueira et al (2013) observed in the equine species that the ratio for both IgA and IgG increased significantly in horses submitted to intestinal obstruction. Barros (2016) also observed an increase in the concentrations of these proteins in PF and a consequent increase in the ratios in donkeys submitted to ovariectomy. The increased concentrations of these immunoglobulins (IgA and IgG) may be related to the local antigenic stimulus triggered by digestive diseases, as well as due to surgical interventions (Peiró et al, 2009).…”

Section: Proteinogrammentioning

confidence: 63%

Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis

Santos

Rego

Afonso

et al. 2021

Semina: Ciênc. Agrár.

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This study aimed to evaluate the hematologic response and the serum and peritoneal fluid (PF) proteinogram of cattle affected by digestive diseases. Twenty-seven animals were distributed in two groups: GI (intestinal diseases) and GII (traumatic reticuloperitonitis, TRP). The animals were previously submitted to a physical exam. Subsequently, blood samples were collected to perform the complete blood count, determine the plasma protein and fibrinogen, and obtain the serum for proteinogram in polyacrylamide gel (SDS-PAGE). Simultaneously, PF was collected to perform physical and chemical evaluation and the electrophoretic profile (SDS-PAGE). ANOVA at the 5% probability level was used to compare the groups. The animals showed signs of apathy, dehydration, and gastrointestinal hypomotility in both groups. However, GI animals showed more significant clinical changes. The blood count of both groups (P > 0.05) showed leukocytosis due to neutrophilia and a regenerative left shift with hyperfibrinogenemia. The proteinogram of both body fluids allowed the identification of proteins albumin (ALB), transferrin (TRF), ceruloplasmin, haptoglobin, ?1-acid glycoprotein (?1-AGP), MW 23000 Da, ?1-antitrypsin, IgA, and IgG. The [PT] PF/[PT] blood serum ratio of each of the identified proteins increased, showing statistical differences between groups (P < 0.05) regarding PT, ALB, TRF, ?1-AGP, and IgG values, with GI animals showing the highest ratio. Intestinal diseases and TRP triggered a systemic and local response characterized by clinical, hematological, and serum and PF proteinogram alterations. The proteins ?1-GPA, haptoglobin, and TRF measured in PF were good inflammation biomarkers and useful as an auxiliary tool for the diagnosis and prognosis of digestive diseases in cattle.

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Section: Proteinogrammentioning

confidence: 63%

Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis

Santos

Rego

Afonso

et al. 2021

Semina: Ciênc. Agrár.

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Pain Assessment and Acute Phase Response in Donkeys Submitted to Inguinal Orchiectomy

nascimento

Graboschii

Fonseca

et al. 2023

Journal of Equine Veterinary Science

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Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization

et al. 2023

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IntroductionHorses submitted to carbohydrate overload can develop laminitis due to changes in cecal pH and microbiota, followed by an increase in transmural absorption of luminal content, including bacterial toxins. In response to acute injury there is hepatic overproduction of several proteins known as acute phase proteins (APP). Few studies have evaluated protein fractionation to characterize the inflammatory response in acute laminitis. The aim of this study was to test the viability of an experimental model to induce acute laminitis, using a single carbohydrate overload, and the influence of a buffering solution on the development of the disease; also, study the kinetics of APP during acute laminitis, as well as the correlation between these proteins and clinical signs associated to this syndrome.MethodsTen healthy horses were divided in a factorial and randomized way into four groups (n = 5): control group (CG), starch group (SG), buffer group (BG), and starch C buffer group (SBG). They were evaluated at seven times (T0h, T4h, T8h, T12h, T24h, T48h, and T72h), which included clinical evaluation and blood sample collection. Total serum protein and albumin concentrations were determined by colorimetry and the other APP by polyacrylamide gel electrophoresis containing sodium dodecyl sulfate and commercial ELISA kits. Data were analyzed by two-way ANOVA, followed by Tukey's test (p < 0.05). The correlation between clinical signs and APP were verified using the Pearson's correlation coefficient.Results and discussion40% of the animals from SG and 60% from SBG developed clinical laminitis. A single administration of buffer solution was not able to prevent clinical signs of laminitis. There was no difference between groups on total serum protein, albumin, serum amyloid A and C-reactive protein concentrations (p > 0.05). Transferrin, considered a negative APP, showed a positive response pattern in SG and SBG. Ceruloplasmin had a positive correlation with Obel grade, heart rate on animals from SGB and number of steps on horses submitted to starch overload (SG and SBG). Ceruloplasmin, α-1-antitrypsin and haptoglobin concentrations increased in SBG, suggesting an inflammatory response in animals of this group. Changes in clinical parameters were also more evident in the SBG, corroborating the protein fractionation findings.

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Resposta inflamatória peritoneal de asininos (Equus asinus) submetidos à ovariectomia por laparotomia e laparoscopia

Cited by 3 publications

References 14 publications

Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis

Hematologic response and serum and peritoneal fluid proteinogram of cattle affected by intestinal diseases and traumatic reticuloperitonitis

Pain Assessment and Acute Phase Response in Donkeys Submitted to Inguinal Orchiectomy

Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization

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