Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples

Makino, Hérica; Sousa, Valéria Régia Franco; Fujimori, Mahyumi; Rodrigues, Juliana Yuki; Dias, Álvaro Felipe de Lima Ruy; Dutra, Valéria; Nakazato, Luciano

doi:10.1590/0103-8478cr20150090

Cited by 7 publications

(7 citation statements)

References 19 publications

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“…We also assessed indirect fluorescent antibody tests (IFATs) at different cut‐off levels against possible clinical and hematologic parameters associated with seropositivity. A population of dogs with suspected CME, treated at a veterinary hospital in Brazil's central‐west region, where E canis infection is considered endemic, were included in the present study 15‐17 . Possible associations among serum and hematologic changes, as functions of different variables, were evaluated by canonical correlation tests, which can highlight variables that stand out when specific antigens are used.…”

Section: Introductionmentioning

confidence: 99%

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Taques

Koiyama

Campos

et al. 2020

Veterinary Clinical Pathol

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BackgroundImmunoreactive tandem repeat proteins (TRPs) in amino acid sequences were identified and employed in the serologic diagnosis of canine monocytic ehrlichiosis (CME).ObjectivesThis study evaluated using TRP19 and TRP36 synthetic protein antigens with enzyme‐linked immunosorbent assays (ELISAs) and compared the results with an indirect fluorescent antibody test (IFAT) to diagnose CME in the serum of dogs with suspected CME.MethodsThe sera of 243 dogs that exhibited clinical and hematologic signs suggestive of CME had IFATs performed. An ELISA with synthetic TRP19 and TRP36 antigens from two E canis genotypes (USTRP36 and BrTRP36) that circulate in Brazil were also performed. Canonical correlations and chi‐square tests were evaluated on the results of these tests.ResultsAmong the 243 dogs, 179 (73.6%) were reactive at an IFAT ≥ 40, 167 (68.72%) reacted at the cut‐off ≥160, and 149 (61.31%) reacted at ≥640. The ELISA revealed that 172 (70.8%) dogs reacted to TRP19 peptide, 163 (67.1%) reacted to USTRP36, and 114 (46.9%) reacted to BrTRP36. The canonical correlation analyses showed that the TRP19 ELISA was the most effective diagnostic method. Serum total protein levels showed strong positive correlations with the USTRP36 peptide. Qualitative analyses revealed an association between the TRP19 peptide and the presence of anemia, thrombocytopenia, and hyperproteinemia. The IFAT results at titers of ≥160 and ≥640 were associated with thrombocytopenia.ConclusionsThe results of this study indicated that the TRP19 peptide was an excellent antigen and that the IFAT was diagnostically important in confirming the diagnosis of CME in serum samples.

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Section: Introductionmentioning

confidence: 99%

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Taques

Koiyama

Campos

et al. 2020

Veterinary Clinical Pathol

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“…Epidemiological studies on the etiologic agents of CME and CCT have already been carried out in several regions of Brazil (LEAL et al, 2015;MAKINO et al, 2016;SOARES et al, 2017;VIEIRA et al, 2018;LOPES et al, 2019). However, there are few studies on the molecular epidemiology of these agents in the northern region of the country.…”

Section: Discussionmentioning

confidence: 99%

“…ROTONDANO et al (2017) detected E. canis in 8.9% of pet dogs in the urban area of the state of Paraíba. MAKINO et al (2016) reported an infection rate of 76.3% in pet dogs in the state of Mato Grosso. In all the studies mentioned above, as well as in our present study, molecular diagnostics were performed in animals without clinical suspicion of CME, indicating that a highly sensitive technique can reveal cryptic cases of infection by this agent.…”

Section: Agent ------------------------------------Group-------------mentioning

confidence: 99%

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

et al. 2019

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The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

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“…The Nested PCR method was used to identify E. canis DNA. In the initial phase, the primers ECC (5´ AGAACGAACGCTGGCGGCAAGC-3´) and ECB (5´ CGTATTACCGCGGCTGCTGGCA-3´) were used to amplify the 16S rRNA gene section, while in the second phase, the E. canis specifi c primer ECAN5 (5-CAATTATTTATAGCCTCTGGCTATAGGA-3´) and the primer HE3 (5´-TATAGGTACCGTCATTATCTTCCCTAT-3´) were used [17][18][19]. The protocol suggested in the studies of Ayan et al (2019) and Ayan et al (2020) was used for the PCR [1,20].…”

Section: Pcr Amplifi Cationmentioning

confidence: 99%

Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province

et al. 2021

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This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province.

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Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples

Abstract: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande

Cited by 7 publications

References 19 publications

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province

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