Molecular Identification of Ehrlichia canis in Rhipicephalus Sanguineus Ticks from Siirt Province

Abstract

This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province.

Low Genetic Diversity of Vector-Borne Haemoparasites in Dogs and Their Ticks Revealed Local and Long-Range Transmission in Peninsular Malaysia

“…Although E. canis is widely distributed globally and has been identified for a long time, little is known about its genetic diversity and strains from geographically distant regions (Hsieh et al, 2010). Recent studies have focused on 16S rRNA genes for molecular work, while there is a dearth of information on other genes (Alhassan et al, 2021;Çelik et al, 2021;Low et al, 2018;Malik et al, 2018;Selim et al, 2021;Sipin et al, 2020). The 16S rRNA gene sequences of E. canis have high similarity despite originating from South America, North America, Asia, Europe, Africa, and the Middle East.…”

Low Genetic Diversity of Vector-Borne Haemoparasites in Dogs and Their Ticks Revealed Local and Long-Range Transmission in Peninsular Malaysia

“…Although E. canis is widely distributed globally and has been identified for a long time, little is known about its genetic diversity and strains from geographically distant regions (Hsieh et al, 2010). Recent studies have focused on 16S rRNA genes for molecular work, while there is a dearth of information on other genes (Alhassan et al, 2021;Çelik et al, 2021;Low et al, 2018;Malik et al, 2018;Selim et al, 2021;Sipin et al, 2020). The 16S rRNA gene sequences of E. canis have high similarity despite originating from South America, North America, Asia, Europe, Africa, and the Middle East.…”