Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with <i>Ehrlichia canis</i>

Taques, Ísis Indaiara Gonçalves Granjeiro; Koiyama, Maíra Fernanda Gonçalves; Campos, Amanda Noeli Silva; Costa, Jackeliny Santos; Hongyu, Kuang; Aguiar, Daniel Moura de

doi:10.1111/vcp.12908

Cited by 7 publications

(18 citation statements)

References 27 publications

(46 reference statements)

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“…Complete clinical data of the dogs in this study were not available for reasons of medical confidentiality. No clinical differences between US or Br genotypes were observed in a hospital population of dogs suffering from CME in an endemic area in Brazil [ 5 ], while clinical differences were observed in dogs infected by different genotypes in another study [ 18 ]. To date, the genetic diversity may be related to the host–pathogen relationship.…”

Section: Discussionmentioning

confidence: 99%

“…ELISA was performed with synthetic peptides corresponding to epitopes from E. canis TRP19 (HFTGPTSFEVNLSEEEKMELQEVS) [ 7 ], USTRP36 (TEDSVSAPATEDSVSAPA) [ 3 ], BrTRP36 (ASVVPEAEASVVPEAEASVVPEAE) [ 4 ] and CRTRP36 (EASVVPAAEAPQPAQQTEDEFFSDGIEA) [ 11 ]. All the ELISA assays were performed according to a previously described protocol [ 4 , 5 ]. Differences between the results found per region and E. canis TRP36 peptides were evaluated by the chi-square test and p ≤ 0.05 was considered significant.…”

Section: Methodsmentioning

confidence: 99%

“…Although they appeared to be located predominantly in the cytoplasm of Ehrlichia, they also appeared to be present outside the morula, suggesting that they may be involved in host cell interactions during infection. TRP19 is a specific target for serological diagnostics [ 3 , 4 , 5 , 6 ] and a strong candidate for vaccine antigen against CME, due to its high degree of conservation and because it is a component of the morula membrane [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil

Taques

Campos

Kavasaki

et al. 2020

Veterinary Sciences

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Tandem repeat proteins (TRPs) are major immunoreactive proteins of Ehrlichia canis, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different E. canis isolates expressed on both reticulate and dense-core cells and observed in the extracellular matrix or associated with the morula membrane. TRP36 is differentially expressed only on the surface of the dense-core form of the bacterium and exhibits more divergence among isolates. The aim of this study was to evaluate the distribution of the American (USTRP36), Brazilian (BrTRP36) and Costa Rican (CRTRP36) genotypes of E. canis in Brazil, using ELISA assays. Serum samples of 814 dogs from 49 municipalities from all over Brazil were analyzed. Our results showed that 33.9% of the samples were reactive to the USTRP36 genotype and 32.6% to the BrTRP36 genotype. The two genotypes appeared to occur equally throughout Brazil, although the frequency of seropositivity was lower in the south than in the country’s other regions. Dogs that reacted to at least one of the synthetic peptides (TRP19 and TRP36) were 456 (56%). A few dogs (n = 5; 0.6%) reactive to the E. canis TRP36 genotype (CRTRP36) were also detected in the northeast and southern regions. We concluded that the American and Brazilian genotypes of E. canis are distributed evenly in Brazil, especially in the tropical region, while the temperate region in the south presented the lowest prevalence rates. This study offers the first report of dogs seropositive for the Costa Rican genotype in Brazil.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil

Taques

Campos

Kavasaki

et al. 2020

Veterinary Sciences

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“…ELISA was performed with synthetic peptides corresponding to epitopes from E. canis TRP19 (HFTGPTSFEVNLSEEEKMELQEVS) [7], USTRP36 (TEDSVSAPATEDSVSAPA) [3], BrTRP36 (ASVVPEAEASVVPEAEASVVPEAE) [4] and CRTRP36 (EASVVPAAEAPQPAQQTEDEFFSDGIEA) [11]. All the ELISA assays were performed according to the protocol described [4][5]. Differences between results found per region and E. canis TRP36 peptides were evaluated by the chi-square test and p≤0.05 was considered significant.…”

Section: Methodsmentioning

confidence: 99%

“…TRP19 is highly conserved among the known E. canis strains. It is a specific target for serological diagnostics [3][4][5][6] and a strong candidate for vaccine antigen for CME, due to its high degree of conservation and because it is a component of the morula membrane [7].…”

Section: Introductionmentioning

confidence: 99%

Geographic Distribution of Ehrlichia Canis Genotypes in Brazil

Taques¹,

Campos²,

Kavazaki³

et al. 2020

Preprint

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Tandem repeat proteins of 36 kDa (TRP36) are major immunoreactive proteins of Ehrlichia canis, which have been used in the serological diagnosis of different genotypes of the microorganism. The aim of this study was to evaluate the distribution of the American (USTRP36), Brazilian (BrTRP36) and Costa Rican (CRTRP36) genotypes of E. canis in Brazil, using ELISA assays. Serum samples of 815 dogs from 49 cities from all over Brazil were analyzed. Our results showed that 33.9% of the samples were reactive to the USTRP36 genotype and 32.6% to the BrTRP36 genotype. The two genotypes appeared to occur equally throughout Brazil, although the frequency of seropositivity was lower in the south than in the country’s other regions. Co-positivity for the American and Brazilian genotypes was also observed in 16% of samples. A few dogs (n=5; 0.6%) reactive to E. canis-TRP36 genotype (CRTRP36) were also detected in the northeast and southern regions. We conclude that the American and Brazilian genotypes of E. canis are distributed evenly in Brazil, especially in the tropical region, while the temperate region in the south presented the lowest prevalence values. This study offers the first report of dogs seropositive for the Costa Rican genotype in Brazil.

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Correlation between canine biochemical analytes and TRP36 ELISA seropositivity for Ehrlichia canis in Brazil

Zorzo

Pereira

Hongyu

et al. 2023

Veterinary Clinical Pathol

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BackgroundSynthetic peptides of tandem repeat proteins (TRPs) have been employed in the serologic analysis of canine monocytic ehrlichiosis (CME) and used in epidemiological studies in Brazil. Based on molecular studies of TRPs, different genotypes of Ehrlichia canis have been described, but data on their pathogenicity remain unknown.ObjectivesTo correlate hepatic, renal, and muscular alterations in relation to different genotypes of E. canis in naturally exposed dogs using enzyme‐linked immunosorbent assay (ELISA) with TRP19 and TRP36 synthetic protein antigens.MethodsTwo hundred serum samples were subjected to ELISA with the antigens of TRP19 and three genotypes (US, Br, and CR) of TRP36 of E. canis circulating in Brazil. Positive sera were evaluated through eight biochemical parameters, and the results were evaluated by principal component analysis and canonical correlation.ResultsELISA revealed that 47 (23.5%) serum samples reacted to the BrTRP36 peptide, 36 (18%) reacted to the TRP19 peptide, and 8 (4%) reacted to the USTRP36 and CRTRP36 peptides separately. The most frequent biochemical alterations observed were for CK (59.4%), ALB (31.8%), GLO (28.9%), TP (28.9%), ALP (26%), urea (24.6%), creatinine (14.4%), and ALT (14.4%). The most prominent diagnostic method in canonical correlation analysis was BrTRP36, followed by TRP19, which correlated with hyperglobulinemia and hypoalbuminemia.ConclusionsAntibodies that reacted against the Brazilian genotype of E. canis correlated positively with hyperglobulinemia and increases in serum urea and creatinine. According to our results, the Brazilian genotype of E. canis is related to the chronic phase of CME.

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Canonical correlative analyses among an enzyme‐linked immunosorbent assay using synthetic peptides, an indirect fluorescent antibody test, and hematologic measurements in dogs infected with Ehrlichia canis

Cited by 7 publications

References 27 publications

Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil

Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil

Geographic Distribution of Ehrlichia Canis Genotypes in Brazil

Correlation between canine biochemical analytes and TRP36 ELISA seropositivity for Ehrlichia canis in Brazil

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