Pine Nanocellulose and Bacterial Nanocellulose Dressings Are Similar in the Treatment of Second-Degree Burn? Experimental Study in Rats

Abstract

Background: Despite all the advances in medicine and the wide variety of dressings available, the treatment of burn wounds still represents an important medical challenge. The pinus cellulose membrane dressing is a biomaterial with characteristics similar to those of bacterial cellulose, but with lower cost. Aim: To evaluate the efficacy of pinus nanocellulose membrane on healing of deep second degree burns in rats and compare with Membracel®. Method: Thirty male Wistar rats were submitted to deep second degree burn in dorse, with boiling water at 97o C for 20 s, generating a 314 mm² area wound. The animals were distributed in three dressing groups (n=10): group 1 - simple gauze; group 2 - bacterial cellulose membrane (Membracel®); and group 3 - pinus cellulose membrane. They were evaluated for 20 days to verify clinical condition, macro and microscopic appearance and wound contraction. Results: All of them remained clinically well with no differences in weight. Crusts were observed in group 1, and none in groups 2 and 3. Regarding to scar contraction, groups 2 and 3 were similar, better than group 1. Microscopic analysis showed predominance of advanced healing degree in groups 1 and 3, and initial in group 2. Mature collagen was predominant in all groups. Conclusion: The pinus nanocellulose membrane is effective in the treatment of experimental second degree burn in rats and its effectiveness is similar to that of the bacterial nanocellular membrane.

Vegetable Cellulose Nanofiber Dressing Aids in the Healing Process of Third-Degree Burns? Study on Rats

“…This suggests the clinical safety of the tested products. These data are compatible with Hakkarainen et al 11 and Coelho et al 7 who tested cellulose membranes of plant origin in wounds on the backs of rats and proved the biocompatibility and atoxicity of the material.…”

“…Muangman et al 16 presented the contribution of bacterial membranes to the healing process of 2 nd degree burns on the face. Coelho et al 7 carried out a study with a vegetal membrane in 2 nd degree deep lesions in rats and obtained results similar to those of the bacterial membrane commercialized in Brazil (Membracel ® ).…”

“…Fantinati et al 8 used a plastic cylinder device resistant to high temperatures and exposed the animal to hot liquid to obtain 3 rd degree burns on the back of the murines. Coelho et al 7 , carried out deep 2 nd degree burns, depositing boiling water in a cut 20 ml syringe, which was kept in contact with the rat’s skin for 20 s. Similar to these studies, the present research also used a device to perform the burn, but in the shape of a funnel, made of material resistant to high temperatures. This device proved to be more practical and safer than the syringe for placing boiling water in the device, reducing the risk of the liquid running and burning the researcher’s hand.…”

“…According to Coelho et al 7 , approximately 80% of medical research uses rodents as experimental animals and in the case of experimental research involving burns and scarring, this is one of the models that gives better results.…”

“…Small perforations were made in the cellulose films with an 18G gauge needle. Before being applied to the wounds of the animals, the membranes were sterilized in an autoclave at 121° C for 30 min, which, under these conditions, the cellulose film does not undergo changes in physical properties 7 .…”