Aloe vera and copaiba oleoresin-loaded chitosan films for wound dressings: microbial permeation, cytotoxicity, and in vivo proof of concept

Genesi, Bianca Pereira; Barbosa, Raquel de Melo; Severino, Patrícia; Rodas, Andrea C.D.; Yoshida, Cristiana Maria Pedroso; Mathor, Mônica Beatriz; Lopes, Patrícia S.; Viseras, César; Souto, Eliana B.; Silva, Classius Ferreira da

doi:10.1016/j.ijpharm.2023.122648

Cited by 15 publications

(2 citation statements)

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“…Regardless of its species, all studies showed that copaiba oil presented a bactericidal and/or bacteriostatic effect in vitro [ 6 ]. Recently, Genesi et al (2023) [ 7 ] reported promising results of chitosan films containing 0.5% copaiba oil for wound healing in adult female rats.…”

Section: Introductionmentioning

confidence: 99%

“…The developed solid lipid nanoparticles containing allantoin and copaiba oil improved the antifungal activity compared to nonencapsulated copaiba oil, showing a similar result compared to commercial antifungal agents [ 35 ]. More recently, copaiba oil was proposed in combination with aloe vera in the production of chitosan films for wound healing [ 7 , 36 ]. The in vivo experiments run in female adult rats confirmed that tested 0.5% copaiba-loaded chitosan films were superior to commercial dressing formulations in their capacity to regenerate epithelium and vessel neoformation.…”

Section: Introductionmentioning

confidence: 99%

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Conventional and PEGylated Liposomes as Vehicles of Copaifera sabulicola

et al. 2023

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Traditional medicine uses resin oils extracted from plants of the genus Copaifera for several purposes. Resin oils are being studied to understand and profile their pharmacological properties. The aim of this work was to prepare and to characterize conventional and pegylated liposomes incorporating resin oils or the hexanic extract obtained from Copaifera sabulicola (copaiba) leaves. The cytotoxic effect of these products was also investigated. Conventional and stealth liposomes with copaiba extract showed similar average diameters (around 126 nm), encapsulation efficiencies greater than 75% and were stable for 90 days. A cytotoxicity test was performed on murine glioma cells and the developed liposomes presented antiproliferative action against these cancer cells at the average concentration of 30 μg/mL. Phytochemicals encapsulated in PEGylated liposomes induced greater reduction in the viability of tumor cells. In addition, bioassay-s measured the cytotoxicity of copaiba resin oil (Copaifera sabulicola) in liposomes (conventional and PEGylated), which was also checked against pheochromocytoma PC12 cells. Its safety was verified in normal rat astrocytes. The results indicate that liposomes encapsulating copaiba oil showed cytotoxic activity against the studied tumor strains in a dose-dependent fashion, demonstrating their potential applications as a chemotherapeutic bioactive formulation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Conventional and PEGylated Liposomes as Vehicles of Copaifera sabulicola

et al. 2023

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Curcumin-Laden Crosslinked Chitosan–PVA Films: The Synergistic Impact of Genipin and Curcumin on Accelerating Wound Closure

Ramdani,

Rao,

Pokharel

et al. 2023

JOM

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Wound dressings play a critical role in healing by maintaining a moist environment and protecting against infection. Here, we fabricate crosslinked films of chitosan–polyvinyl alcohol–genipin–curcumin to investigate the synergistic wound-healing effect of genipin and curcumin. The chemical bonding, morphology, strength, water retention capacity, curcumin release characteristics, and cytotoxicity of the films were investigated. The results showed a 192% increase in tensile strength and good water retention, and the infrared analysis and scanning electron microscopy micrographs confirmed genipin crosslinking and the presence of curcumin in the films, whose morphology was uniform and continuous. The curcumin-loaded films were found to have insignificant cytotoxicity against 3T3 fibroblast cells, proving their biocompatibility. The curcumin dissolution tests determined the amount of curcumin released from the films with time, and assessed the release characteristics to be sustained at all pH levels. In vivo trials were carried out on rodents to evaluate the wound-healing effects of the films, and the results showed that the curcumin-incorporated crosslinked films accelerated wound healing compared to the uncrosslinked and curcumin-absent films, confirming their effectiveness for wound healing.

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