Different Protocols of Postconditioning Does Not Attenuate Mesenteric Ischemia-Reperfusion Injury After Short-Term Reperfusion

Brito, Marcus Vinicius Henriques; Yasojima, Edson Yuzur; Machado, Andressa A.; Silveira, Matheus Paiva Pacheco Reis; Teixeira, Renan Kleber Costa; Yamaki, Vítor Nagai; Costa, Felipe Lobato da Silva

doi:10.1590/0102-6720201700010008

Cited by 3 publications

(5 citation statements)

References 23 publications

(44 reference statements)

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“…In this model, weight loss was more related to the length of the removed small bowel rather than the exclusion of the ileocecal valve. Also, all animals survive until experimental end, making this model relevant to study long-term effect of short bowel syndrome and to create translational experiments of new intestinal transplantation models 3 , 5 , 6 , 21 using rats recipients with short bowel syndrome, like in clinical management.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Ileocecal Valve Removal in a Model of Short Bowel Syndrome

Soler¹,

Lee²,

D’Albuquerque³

et al. 2019

ABCD, arq. bras. cir. dig.

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Background:Short bowel syndrome is a harmful condition that needs experimental research. Aim:To assess the impact of the ileocecal valve removal in a model of short bowel syndrome, in order to investigate the evolution of the colon under this circumstance. Method:Fifteen Wistar rats were equitable divided into: Control (Sham), Group I (70% enterectomy preserving ileocecal valve) and Group II (70% enterectomy excluding ileocecal valve). After enterectomy was performed jejunoileal or jejunocecal anastomosis and sacrificed the animals on 30th postoperative day for histomorphometric study of the colon. During this period, was observed the clinical evolution of the animals weekly including body weight measurement. Results:Group I and II presented progressive loss of weight. In Group I was observed diarrhea, perineal hyperemia and purple color of the colon during autopsy. Histomorphometry assay showed hypertrophy and hyperplasia of colon mucosa in Group I. In Group II the colon wall was thicker due to hypertrophy and muscular hyperplasia, and in mucosa vascular proliferation and inflammatory infiltrate were intense. Conclusion:This short bowel syndrome model is relevant and achieve 100% of survival. Animal’s weight loss was not altered by the presence or exclusion of the ileocecal valve. Animals with 70% of small bowel removal and presence of the ileocecal valve attained a better clinical evolution and histological colon adaptation than those without ileocecal valve.

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Section: Discussionmentioning

confidence: 99%

The Effect of Ileocecal Valve Removal in a Model of Short Bowel Syndrome

Soler¹,

Lee²,

D’Albuquerque³

et al. 2019

ABCD, arq. bras. cir. dig.

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“…After exposure of an organ to ischemic insult, restoration of blood flow is the only way to stop the ischemic cascade. However, reperfusion may result in reactive oxygen species formation and increase in inflammatory reaction which can induce tissue damage greater than ischemia itself [28,45] . Renal ischemia reperfusion injury (I/R) is a common result of clinical procedures such as renal resection, transplantation, trauma, sepsis and shock [2,3] .…”

Section: Discussionmentioning

confidence: 99%

“…This is in line with previous reports indicated that, the protective effect of EPO was still apparent even when administration is delayed up to 6h after the onset of injury [57] . Although less effective, EPO post treatment has greater clinical applicability than EPO pre-treatment because the ischemic insult is difficult to be predicted [28] .…”

Section: Discussionmentioning

confidence: 99%

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Potential Hepatic Protection by Erythropoietin and Ischemic Conditioning in a Rat Model of Renal Ischemia-Reperfusion Injury

Ibrahim¹,

Megahed

Fathy³

et al. 2018

The Medical Journal of Cairo University

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Background: Renal ischemia reperfusion injury is a common consequence of many clinical conditions including renal transplantation, sepsis, trauma, and shock and usually leads to acute kidney injury. Its adverse effects extend to other organs including the liver leading to remote organ injury. Erythropoietin and ischemic conditioning have an antiinflammatory, anti-oxidant and anti-apoptotic effects and were reported to have a protective effect against ischemia reperfusion injury in different organs. Aim of the Study:To evaluate the possible protective effect of erythropoietin and ischemic conditioning on the liver following renal ischemia reperfusion injury and to declare some of the possible mechanisms involved. Material and Methods:This study was conducted on 66 adult male albino rats divided into 6 equal groups: Group I: Sham group; Group II: Renal ischemia reperfusion group (I/R); Group III: Erythropoietin pre-treatment renal ischemia reperfusion group (EPO pre-I/R); Group IV: Erythropoietin post-treatment renal ischemia reperfusion group (EPO post-I/R); Group V: Ischemic pre-conditioning-renal ischemia reperfusion group (IPC) and Group VI: Ischemic postconditioning-renal ischemia reperfusion group (IPOC).Results: Group II showed significant increase in serum creatinine, blood urea, blood urea nitrogen, AST and ALT levels with significant increase in renal and hepatic injury score indicating renal and hepatic injury following renal ischemia reperfusion. In the same group, a significant increase in serum IL6, TNFa , MDA and a significant decrease in serum SOD were found. Both group III and IV revealed a significant decrease in serum creatinine, blood urea, blood urea nitrogen, AST, ALT, renal and hepatic injury score. Levels of IL6, TNFa , and MDA significantly decrease and SOD significantly increased in both groups relative to group II and these changes were significantly higher in group III relative to group IV. In group V and VI, a significant decrease in serum creatinine, blood urea, blood urea nitrogen and renal injury score was found in both groups, while serum AST, ALT and hepatic injury score were significantly decreased in group VI but remained insignificantly changed in group V versus group II. Serum IL6 and TNFa significantly decreased in both groups, while MDA significantly decreased and SOD significantly Correspondence to: Maha A. Fathy, Email: y_maha_m@hotmail.com increased in group VI only and no significant change was noticed in group V versus group II.Conclusion: Erythropoietin pre and post treatment significantly improved renal and hepatic function and morphology following renal ischemia reperfusion injury with better results for erythropoietin pre-treatment. Ischemic post-conditioning induced significant decrease in renal and hepatic injury, while ischemic pre-conditioning provided renal protective effect which didn't extend to involve the liver.

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“…Immediately after, a 2 cm ileum sample distant 3 cm from the cecum valve was harvested. This fragment was fixed in 10% buffered formaldehyde, and underwent histological processing; the slides were stained with hematoxylin-eosin and examined under light microscopy to determine the degree of injury based on the scale of Chiu et al 7 and villous length measurements were done by measuring ten well-oriented villous 5 (cut across its length) in each of the animals.…”

Section: Methodsmentioning

confidence: 99%

Effect of Copaiba Oil in Intestinal Mucosa of Rats Submitted to Hypovolemic Shock

Teixeira

Costa

Calvo

et al. 2019

ABCD, arq. bras. cir. dig.

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Background: Hypovolemic shock is a common disease in polytrauma patients and may develop ischemia in various organs, increasing morbidity and mortality. The bowel is usually most affected by this condition. Aim: To evaluate the effects of copaiba oil on the intestinal mucosa’s injury of rats submitted to hypovolemic shock. Method: Fifteen rats were divided into three groups: sham - simulated surgery; ischemia - animals submitted to hypovolemic shock; and copaiba - animals submitted to hypovolemic shock previously treated with copaiba oil. Mean blood pressure, arterial blood gas after shock induction, degree of intestinal lesion and villus length were evaluated. Results: The sham presented the lowest values of lactate and PaCO2 and the highest values of mean arterial pressure, pH and bicarbonate in relation to the other groups. The degree of mesenteric lesion was zero in the sham group; 3.00±1.00 in the ischemia group; and 3.00±0.71 in the copaiba group. The villus length was 173.60±8.42 in the sham, 142.77±8.33 in the ischemia and 143.01±9.57 in the copaiba group. There was a significant difference between the sham and the other groups (p<0.05); however, there not significant difference between groups Ischemia and copaiba. Conclusion: Administration of copaiba oil did not reduce the intestinal mucosa lesion of rats after hypovolemic shock.

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Different Protocols of Postconditioning Does Not Attenuate Mesenteric Ischemia-Reperfusion Injury After Short-Term Reperfusion

Cited by 3 publications

References 23 publications

The Effect of Ileocecal Valve Removal in a Model of Short Bowel Syndrome

The Effect of Ileocecal Valve Removal in a Model of Short Bowel Syndrome

Potential Hepatic Protection by Erythropoietin and Ischemic Conditioning in a Rat Model of Renal Ischemia-Reperfusion Injury

Effect of Copaiba Oil in Intestinal Mucosa of Rats Submitted to Hypovolemic Shock

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