Esterilização Química E Agentes Geleificantes Alternativos Na Propagação Vegetativa in Vitro Do Abacaxizeiro ‘Vitória’

Abstract

RESUMO-O objetivo deste trabalho foi avaliar a eficiência da esterilização química do meio de cultura e de agentes geleificantes alternativos e sua influência no desenvolvimento in vitro e ex vitro de plantas de abacaxizeiro 'Vitória'. O meio de cultivo utilizado foi composto pelos sais do MS e vitaminas de White, com 30 g L -1 de sacarose e 100 mg L -1 de mioinositol. Foram realizadas duas formas de esterilização dos meios de cultivo e das vidrarias: em autoclave a 121°C e com solução de hipoclorito de sódio (NaClO) a 0,003% para enxágue das vidrarias e 0,0003% no meio de cultivo. Os agentes geleificantes testados foram:). Após 30 dias de cultivo in vitro, constatou-se que o NaClO e os agentes geleificantes alternativos não interferiram no crescimento e no desenvolvimento das mudas. Parte das mudas de cada tratamento foi aclimatizada durante 90 dias, após os quais se verificou que não houve influência dos agentes geleificantes no crescimento destas, e, para a maioria dos parâmetros avaliados, as mudas oriundas dos tratamentos envolvendo a esterilização química apresentaram resultados semelhantes ou superiores às dos meios autoclavados. Termos para indexação: Ananas comosus var. comosus, hipoclorito de sódio, amido de milho, amido de mandioca, micropropagação. CHEMICAL STERILIZATION AND ALTERNATIVE GELLING AGENTS IN THE IN VITRO VEGETATIVE PROPAGATION OF PINEAPPLE 'VITÓRIA'ABSTRACT -The objective of this study was to evaluate the efficiency of chemical sterilization of the culture medium and alternative gelling agents and their influence on development in vitro and ex vitro plants of pineapple 'Vitória'. The culture medium used was composed of MS salts and White vitamins, with 30 g L -1 of sucrose and 100 mg L -1 of myo-inositol. Two forms of sterilization of the media culture and the glassware were performed: in autoclave at 121°C and with solution of sodium hypochlorite (NaClO) at 0.003% to rinse the glassware and 0.0003% in the culture medium. The gelling agents tested were: M1: agar (6.0 g L ). After 30 days of in vitro cultivation was found that the NaClO and alternative gelling agents did not affect the growth and development of plantlets. Part of the plantlets of each treatment was acclimatized during 90 days, after which it was found that there was no influence of gelling agents on plantlets growth. For most of the parameters evaluated, the plantlets derived from treatments involving the chemical sterilization showed results similar or superior to the autoclaved media.

Costreductionin the micropropagation of Solanum lycopersicumL. var.cerasiforme

“…Moreover, the different types of sterilization did not affect plant growth. The use of sodium hypochlorite as a sterilizing agent was efficient for decontaminating the medium (Table 4), as observed in other studies with pineapple (TEIXEIRA et al, 2006, OLIVEIRA et al, 2015, sequoia (RIBEIRO et al, 2011), sugarcane (TIWARI et al, 2012) and the "cabeça-de-frade" cactus (RESENDE et al, 2021). In addition, the use of sodium hypochlorite is a viable and efficient option in the sterilization of the culture medium, since it has a lower toxicity index when compared to other products, such as mercury (TEIXEIRA et al, 2006;2008).…”

In Vitro Seed Germination and Plant Growth of “Cabeça-De-Frade” (Cactaceae)

“…Brondani et al (2013) warn that active chlorine added to a culture medium can both cause damage to tissues and be beneficial depending on the concentration. Oliveira et al (2015) observed that sterilization with NaOCl allowed similar or superior results compared to autoclaving for the analyzed variables for Ananas comosus var. comomus.…”

Concentrations of cytokinin and the use of agricultural residues in the in vitro propagation media of highbush blueberry

“…In order to reduce the costs of micropropagation, research to replace reagents such as agar with alternative solidifying materials for culture media that also allow for adequate development of the explants has been conducted. For example, GOLLE et al (2010) experimented with corn starch, vermiculite, filter paper, and hydrophilic cotton for micropropagation of the loblolly pine (Pinus taeda), CURTI & REINIGER (2014) tested vermiculite, Plantmax ® , and fine sand in yellow poinciana (Peltophorum dubium), OLIVEIRA et al (2015aOLIVEIRA et al ( , 2015b tested corn starch and cassava starch in pineapple (Ananas comosus), andRODRIGUES et al (2016) andNADAL et al (2018) assessed the use of coconut fiber, carbonized rice husk, and in natura rice husk for propagation of golden rain orchid (Oncidium baueri).…”