Costreductionin the micropropagation of Solanum lycopersicumL. var.cerasiforme

Abstract

The aimof this study was to establish a low-cost alternative protocol for micropropagation ofSolanum lycopersicumL. var.cerasiforme,popularly known as cherry tomato. In the in vitroestablishment, culture mediacontaining Laboratory Reagent-grade (LR)and commercialsucrose and varied concentrations of corn starch and agarwere tested. The replacement of thermal sterilization, usingautoclave,withchemicalsterilization,adding sodium hypochlorite (2%) in the medium, was also evaluated. In the multiplication stage,the mediumwas supplemented with agar and/or corn starch and commercial sucrose.Forrooting, agrowth regulator-free medium withcommercialsucrose supplemented with agar and/or starch was used. Themicroplants were thentransplanted into plasticcontainerscontainingonlygarden substrateandsubsequentlyacclimatized in a greenhouse.The results make it possibleto conclude that the reduction of costs in the micropropagation of cherry tomatocan beobtained by replacing LRsucrose with commercial sucrose,and by the use of chemical sterilization of the culture medium withsodium hypochlorite. The replacement of agar withcorn starch can be done partially, in the stages of establishment and multiplication,and totally, during rooting

Gelation of Culture Medium with K-Carrageenan Improves and Reduces the Cost of in vitro Propagation of Comanthera mucugensis (Giul.) L. R. Parra & Giul

“…The reduction in the cost of micropropagation of plants through the partial and total substitution of agar has been reported in several studies and has generated promising results [14,[33][34][35]. The main low-cost alternatives are: corn starch, sago, cassava starch and isabgol.…”

“…Over the years, several types of gelling agents have been developed and incorporated into in vitro culture protocols, including with superior results compared to agar [12][13][14]. For example, gelation with κcarrageenan, a polysaccharide also obtained from red macroalgae composed of galactose and ester sulfate subunits, has been reported in studies involving direct and indirect organogenesis, and somatic embryogenesis in several plant species [9,15,16].…”