DIVERSIDADE GENOTÍPICA E PATOGÊNICA DE Colletotrichum musae NO ESTADO DE PERNAMBUCO

Santos, Paulo Cézar das Mercês; Lima, Waléria Guerreiro; Bezerra, Cintia de Sousa; Michereff, Sami Jorge; Câmara, Marcos Paz Saraiva

doi:10.1590/0100-2945-034/14

Cited by 7 publications

(4 citation statements)

References 19 publications

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“…Similar results were also observed by Santos et al (2015) when evaluated genotypic and pathogenic diversity of Colletotrichum musae in the state of Pernambuco/Brasil using ISSR, and observed a tendency of clustering isolates from the same geographical location.…”

Section: Discussionsupporting

confidence: 85%

Genetic variability of Colletotrichum sublineolum through ISSR markers

Guimarães

Barelli

Corrêa

et al. 2021

RSD

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The occurrence of diseases is a limiting factor in the development of sorghum crop. Among the diseases that causes losses in sorghum production, anthracnose is the main and most severe, mainly by the genetic variability of the pathogen. In this context, the aim of this study was to evaluate the genetic variability of Colletotrichum sublineolum isolates. DNA were extracted from 56 monosporic isolates of C. sublineolum using a DNA extraction kit, and to perform the analysis of genetic diversity of the isolates were used ISSR primers. After amplification, it was determined the polymorphic information content (PIC), allelic frequency, UPGMA and Tocher clustering analyzes and, using software Structure, the genetic structure. According to the descriptive analysis of the genetic variability of C. Sublineolum isolates, primer AP1 presented the higher value of polymorphic information content (PIC). The higher allelic frequency was observed in loci 06, 09, 10, and 24, and the lowest in locus 02. As for the clustering method, it was observed a tendency of grouping C. sublineolum isolates according the geographic origin and, in addition to demonstrating the genetic variability between the C. sublineolum isolates, it was observed the occurrence of introgression among the isolates.

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Section: Discussionsupporting

confidence: 85%

Genetic variability of Colletotrichum sublineolum through ISSR markers

Guimarães

Barelli

Corrêa

et al. 2021

RSD

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“…It is one of the most destructive diseases of sweet and chili peppers in tropical and subtropical regions, and its spread occurs mainly in crops that are grown outdoors during periods of high temperature and humidity. The fungus produces conidia (spores) as reproductive structures, which are released and disseminated by splashes of rain, wind, and insects, among other vectors [3].…”

Section: Introductionmentioning

confidence: 99%

Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

et al. 2019

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There are several phytosanitary problems that have been causing serious damage to the Capsicum crops, including anthracnose. Upon attack by certain pathogens, various protein molecules are produced, which are known as proteins related to pathogenesis (PR proteins), including antimicrobial peptides such as protease inhibitors, defensins and lipid transfer proteins (LTPs). The objective of this work is to identify antimicrobial proteins and/or peptides of two genotypes from Capsicum annuum fruits infected with Colletotrichum gloeosporioides. The fungus was inoculated into Capsicum fruits by the deposition of a spore suspension (106 conidia ml−1), and after 24 and 48 h intervals, the fruits were removed from the humid chamber and subjected to a protein extraction process. Protein analysis of the extracts was performed by tricine gel electrophoresis and Western blotting. The distinctive bands between genotypes in the electrophoresis profiles were subjected to mass spectrometry sequencing. Trypsin inhibition assays, reverse zymographic detection of protease inhibition and β-1,3-glucanase activity assays were also performed and extracts were also tested for their ability to inhibit the growth of C. gloeosporioides fungi ‘in vitro’. There were several low molecular weight proteins in all treated samples, and some treatments in which antimicrobial peptides such as defensin, lipid transfer protein (LTP) and protease inhibitor have been identified. It was shown that the green fruits are more responsive to infection, showing the production of antimicrobial peptides in response to injury and inoculation of the fungus, what did not occur in ripe fruits under any treatment.

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“…Este resultado pode ser devido à maior quantidade de isolados analisados desta procedência, evidenciando maior número de loci polimórficos ou ainda pela ocorrência de fluxo gênico. SANTOS et al (2015), estudaram a diversidade genotípica e patogênica de C. musae, observaram que os índices de diversidade genotípica indicaram a ocorrência de fluxo gênico, provavelmente, devido ao fungo ser transportado via material de propagação e não haver fiscalização sanitária entre regiões.…”

Section: Caracterização Molecularunclassified