Simultaneous quantification of six flavonoids in four Scutellaria taxa by HPLC-DAD method

Bardakçı, Hilal; Acar, Ebru Türköz; Kırmızıbekmez, Hasan

doi:10.1016/j.bjp.2018.09.006

Cited by 14 publications

(11 citation statements)

References 12 publications

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“…However, scutellarin is the one that is characteristic and present in all species studied by us, regardless of their habitat. In the plant material of S. albida from both regions, in addition to significant amounts of scutellarin, we also find the phenylethanoid glycoside-verbascoside, which was also identified by Matsa et al [ 27 ] as well as luteoline—determined by Bardakci et al [ 28 ] in S. albida subsp velenovskyi from the Karabük region of Turkey.…”

Section: Discussionsupporting

confidence: 82%

Metabolite Profile and Antioxidant Activity of Some Species of Genus Scutellaria Growing in Bulgaria

Georgieva

Stoyanov

et al. 2020

Plants

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Until now, the interest to plants from genus Scutellaria in Bulgaria has been focused mainly on the terpenes in them. The purpose of this study is to enrich the information on the composition of the Bulgarian Scutellaria species in terms of both polyphenolic content as well as primary metabolites such as mono-, oligosaccharides and organic acids. An aerial part of three Scutellaria species growing in four low mountain regions of Southern Bulgaria was used. The flavonoids scutellarin, baicalin, baicalein, wogonin, wogonoside, luteolin, chrysin and a caffeoyl phenylethanoid glycoside-verbascoside have been identified via HPLC in different extracts from Scutellaria altissima, Scutellaria albida and Scutellaria galericulata. The antioxidant activity of the extracts has been evaluated. The Scutellaria altissima from Mezek and Scutellaria galericulata from Parvenets we studied, which are the richest in flavonoids (represented mainly by baicalin, scutellarin and wogonoside), show the highest Oxygen Radical Absorption Capacity. Hydroxyl Radical Averting Capacity of Scutellaria albida from Mezek and Scutellaria altissima from Bachkovo is the most pronounced, probably due to the content of scutellarin and luteolin and chrysin, respectively. Antioxidant activity of aqueous, methanolic and 70% and 96% ethanol extracts were also determined by the electrochemical method.

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Section: Discussionsupporting

confidence: 82%

Metabolite Profile and Antioxidant Activity of Some Species of Genus Scutellaria Growing in Bulgaria

Georgieva

Stoyanov

et al. 2020

Plants

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“…Recent studies have reported a LOD for six flavonoids in plants ranging from around 0.058–1.88 µg/mL [ 24 ]; however, these limits are not enough for pharmacokinetic studies where the sample is only a few microliters of serum. Bhatt and coworkers [ 25 ] reported a LOD for hesperidin of 60 ng/mL using MS/MS.…”

Section: Resultsmentioning

confidence: 99%

Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

et al. 2020

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The purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µL) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimization process allowed for high sensitivity with detection limits of approximately picogram order using an electrospray ionization (ESI) source in negative mode and an experiment based on multiple reaction monitoring (MRM). The validation parameters showed excellent linearity and detection limits, with a precision of less than 8% and a recovery of over 90%. This methodology was applied to compare the pharmacokinetic parameters for the administration of hesperidin and naringenin in individual form or in the form of a mixture. The results showed an absence of significant effects (p > 0.05) for Tmax and Cmax; however, the AUC presented significant differences (p < 0.05) for both flavonoids when administered as a mixture, showing an improved absorption ratio for both flavonoids.

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“…The acetylcholinesterase, butyrylcholinesterase and tyrosinase inhibitory activities of methanol extracts and the antioxidant activity of methanol and ethyl acetate extracts were investigated by DPPH and FRAP experiments in a study by Şenol et al [25]. In recent years, İçen [29]. In this study, we planned to investigate the antibacterial and antifungal activity of methanol extracts of 15 subspecies of S. orientalis using broth microdilution method.…”

Section: Introductionmentioning

confidence: 99%

Comparison of The Antimicrobial Activity of Some Scutellaria orientalis L. Taxa Growing in Turkey

Yılmaz

Şimşek

Altanlar

et al. 2020

International Journal of Secondary Metabolite

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Scutellaria species are commonly used in the treatment of various diseases in traditional medicine. One of the members of Lamiaceae, the genus Scutellaria L. has approximately 471 species on earth. The genus is represented by 39 taxa in Turkey which 17 of them are endemics. In this study, plant samples of 15 S. orientalis subspecies from different regions of our country were collected and methanol extracts were prepared from aerial parts. In vitro antimicrobial activity of these extracts against three gram positive and three gram negative bacteria and against a yeast using broth microdilution method. Methanol extracts of S. orientalis taxa were found to have moderate to low antimicrobial activity compared to the literature.

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Simultaneous quantification of six flavonoids in four Scutellaria taxa by HPLC-DAD method

Cited by 14 publications

References 12 publications

Metabolite Profile and Antioxidant Activity of Some Species of Genus Scutellaria Growing in Bulgaria

Metabolite Profile and Antioxidant Activity of Some Species of Genus Scutellaria Growing in Bulgaria

Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

Comparison of The Antimicrobial Activity of Some Scutellaria orientalis L. Taxa Growing in Turkey

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