Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil

Abstract

In the Staphylococcus spp. analyzed, the ermC gene was found more often than the ermA, although the iMLSB phenotype had been less frequent than the cMLSB. It was important to perform the D test for its detection to guide therapeutic approaches.

Phenotypic Detection of Β-Lactam Antibiotics, Methicillin and Inducible Clindamycin Resistance Among Bacterial Isolates in Patients with Otitis Externa

“…It is noteworthy to mention that among 14 MDR CoNS isolates, 3 (16.7%) strains were D-zone test positive and 2 (14.3%) strains were resistant to the methicillin group. According to studies conducted by Niedja (Brazil) and Bhatt (India), none of the CoNS isolates were D-zone test positive (5,27).…”

“…The most common resistance mechanism occurs within Staphylococci that is due to the erm gene, encoding RNA methylase through plasmids (4). Clindamycin is prescribed for the treatment of skin and soft tissue infections, in cases of bacterial antibiotic resistance against methicillin and penicillin allergy (5). Methicillin is a derivative of the penicillin group and resistant to penicillinase.…”

Antibiotics resistance patterns of Panton-Valentine leukocidin-positive methicillin-resistant staphylococci isolated from clinical samples in Abidjan, Cte dIvoire

“…The frequency of cMLS B and iMLS B of the present study was lower than those found in previous studies. Two other previous Brazil studies (Bottega et al, 2014;Pereira et al, 2016) have documented the rate of cMLS B and iMLS B in clinical MRSA strains ranging from 14.3 to 37.9% and 2.1 to 4.9%, respectively; while the KTG-R phenotype was not detected. In India, the MLS B phenotypes of MRSA in clinical samples were higher than those reported in this study.…”

“…Reporting the susceptibility to clindamycin without verifying the presence of inducible resistance, may lead to inadequate therapy with this drug. In contrast, a negative result for inducible resistance to clindamycin, confirms the susceptibility of this antimicrobial, providing a very good therapeutic option (Pereira et al, 2016). Clindamycin is the preferred agent for the treatment of MRSA due to its excellent pharmacokinetic properties, such as optimal tissue penetration and accumulation in abscesses.…”

“…The susceptibility testing was carried out by culturing strains on Mueller-Hinton agar (Bio-rad, Marne-la-coquette, France). Antibiotics used for susceptibility testing included penicillin 6 µg, cefoxitin 30 µg, ciprofloxacin 5 µg, norfloxacin 5 µg, gentamicin 10 µg, kanamycin 30 µg, tobramycin 10 µg, netilmicin 10 µg, (Kumari et al, 2016;Pereira et al, 2016). Multidrug resistance (MDR) was defined as resistance to at least three distinct antimicrobial classes or being MRSA (Magiorakos et al, 2011).…”