In this study, the antioxidative effects of Aronia melanocarpa fruit and leaf extracts were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities (FSC50) of the ethylacetate and aglycone fractions of fruit extracts were 16.29 μg/mL, and 12.29 μg/mL, respectively. The free radical scavenging activity of fruit extract was higher than that of leaf extracts. Reactive oxygen species (ROS) scavenging activities (OSC50) of the ethylacetate and aglycone fractions of fruit extracts on ROS generated in Fe
3+-EDTA/H2O2 system using the luminol-dependent chemiluminescence assay showed 2.86 μg/mL, and 1.80 μg/mL, respectively. ROS scavenging activity of the aglycone fraction of fruit extracts was similar to that of L-ascorbic acid (1.50 μg/mL). The ROS scavenging activity of fruit extracts was higher than that of leaf extracts. The cellular protective effects of aglycone fraction of fruit extracts (τ50 = 72.3 min) on the 1 O2-induced cellular damage of human erythrocytes especially were increased in a concentration dependent manner (5 ~ 50 μg/mL). τ50 (72.3 min) of the aglycone fraction showed 1.9 times higher than (+)-α -tocopherol (38 min), known as lipophilic antioxidant at 10 μg/mL. These results incidicate that A. melanocarpa fruit extracts have higher antioxidant effects than leaf extracts and could be applicable to functional cosmetics materials for antioxidants by protecting skin exposed to solar UV radiation against ROS including 1 O2.
In this study, Polygomun aviculare L. (P. aviculare L.) extract loaded elastic liposomes (ELPs) were investigated to enhance the transdermal delivery of P. aviculare L. extract composed of various flavonoids. ELPs were composed of egg phospholipids (PC) and edge activator (Tego ® care 450) and the physical properties and in vitro permeation studies of ELPs were performed. Particle size ranged from 148.1 to 262.2 nm and deformability index was recorded as 11.5~25.4. Loading efficiency was from 53.1 to 66.3%. In vitro skin permeation studies using Franz diffusion cell demonstrated that ELP-4 having ratio of 85:15 for PC to Tego ® care 450 exhibited the higher skin permeability than ELP-1, the general liposome without Tego ® care 450. It was visually seen by fluorescence image restoration microscopy. The findings suggest that ELP-4 selected as the optimal formulation could be used as useful formulation for transdermal delivery of the extract.
In this study, the antioxidative effects and component analysis for the extracts of Rumex acetosa L. were investigated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from dried R. acetosa L.. Free radical scavenging activities (1,1-diphenyl-2-picrylhydrazyl) size of, in the order of aglycone fraction > ethyl acetate fraction > 50% ethanol extract, aglycone fraction (45.10 µg/mL) showed the highest radical scavenging activity. Reactive oxygen species (ROS) scavenging activity (total antioxidant capacity, OSC 50 ) on ROS generated in Fe
3+-EDTA/H 2 O 2 system was also, in the order of ethyl acetate fraction> aglycone fraction> 50% ethyl acetate fraction, ethyl acetate fraction (2.68 µg/mL) was shown a great antioxidant capacity. The total antioxidant capacity of the ethyl acetate fraction was found to be greater than L-ascorbic acid, known as a typical hydrophilic antioxidant (6.88 µg/mL). The cellular protective effects of R. acetosa L. extracts on the 1 O 2 -induced cellular damage of
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