Five sequence characterized amplified region (SCAR) DNA markers were reevaluated at substantially higher annealing temperatures than originally reported; four were polymorphic among nine rootstocks tested. Four new informative SCAR markers also are reported, based on redesigning primers from previously cloned random amplified polymorphic DNA (RAPD) markers. Based on the eight polymorphic markers, rootstocks MG 420A, MG101-14, Richter 99, Couderc 3309, and Kober 5BB were distinguishable. Riparia Gloire and Couderc 1616 could be distinguished from the others, but not from one another, and SO4 and 5C also could be distinguished from the others, but not from one another.
The Street Museum is the first nationwide initiative in Wuhan to apply new museology to the protection planning of historical streets. The Lihuangpi Road Street Museum is the first street museum in Wuhan. It has played an active role in protecting historical relics, showing the unique architectural style and spatial texture of Lihuangpi Road, reflecting urban diversity, improving the living environment, and promoting the development of cultural and leisure industries effect. However, problems such as irrationality in planning and design, low follow-up investment, lack of management and supervision, and inactive participation of community residents have affected its effectiveness. Through in-depth investigations and interviews, this article summarizes the practical results and problems of the Lihuangpi Road Street Museum since its establishment, and proposes targeted opinions, with a view to providing further reference for the protection and planning of historical blocks in other cities.
A total of eight random amplified polymorphic DNA (RAPD) markers were generated in a screen of 77 primers of 10-base length and were detected reproducibly among nine different grape (Vitis) rootstocks. Occasional failed amplifications could not be explained rationally nor easily corrected by systematic replacement of individual reaction components. In an effort to improve their reliability, the RAPD markers were cloned, their termini sequenced, and new sequence-specific primer pairs were synthesized based on addition of 10 to 14 bases to the 3' termini of the original 10-mers. Six pairs of the new primers were evaluated at their optimal and higher-than optimal annealing temperatures. One primer pair amplified a product the same size as the original RAPD marker in all rootstocks, resulting in loss of polymorphism. Post-amplification digestion with 7 different restriction endonucleases failed to reveal restriction site differences. Three primer pairs amplified an unexpected length variant in some accessions. Two other pairs of primers amplified a number of unexpected bands. Better approaches for exploiting the sequence differences that account for the RAPD phenomenon will be discussed.
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