Identification of Grape (Vitis) Rootstocks Using Sequence Characterized Amplified Region DNA Markers

徐虹,; Bakalinsky, Alan T.

doi:10.21273/hortsci.31.2.267

Cited by 19 publications

(6 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1990), several studies have shown that the RAPD assay is unusually sensitive to changes in reaction conditions and that even some of the banding patterns could be DNA polymerase dependent (Black 1993; Wolf et al . 1993; Xu & Bakalinsky 1996). These problems can be partly solved by converting RAPD markers into SCAR markers (Paran & Michelmore 1993).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of sequence amplified characterized region (SCAR) markers of Helicoverpa armigera: a new polymerase chain reaction‐based technique for predator gut analysis

Agustí¹,

Vicente²,

Gabarra³

1999

Molecular Ecology

107

104

View full text Add to dashboard Cite

A method is described for the development of DNA markers for detection of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) in predator gut analysis, based on sequence characterized amplified regions (SCARs) derived from a randomly amplified polymorphic DNA (RAPD) band. A 1200-bp DNA fragment of H. armigera, absent in the predator band pattern and in other closely related prey species, was identified by RAPD analysis. This fragment was cloned and its extremes sequenced to design extended strand-specific 20-mer oligonucleotide primers. Three pairs of SCAR primers, which amplified three different DNA fragments, were used to study the effect of fragment length on detection of prey in the predator gut. Using the pair of primers that amplified the longest fragment of H. armigera DNA, a single band of 1100 bp was obtained, but its detection was not possible in the predator gut. Detection of the ingested prey was possible with the other two pairs of SCAR primers, obtaining bands of 600 and 254 bp, respectively. Detection of H. armigera DNA in the gut of the predator Dicyphus tamaninii was evaluated immediately after ingestion (t = 0) and after 4 h. Detection of H. armigera DNA after 4 h was only possible using the pair of primers that amplified the shortest fragment (254 bp). The test for specificity, using these last pair of primers, showed that H. armigera was the only species detected. The detection threshold was defined at a 1:8192 dilution of a H. armigera whole egg in all samples.

show abstract

Section: Discussionmentioning

confidence: 99%

“…1996; Ohmori et al . 1996; Xu & Bakalinsky 1996) and animal species (Cargill et al . 1995; Gutiérrez‐Adán et al .…”

Section: Introductionmentioning

confidence: 99%

Development of sequence amplified characterized region (SCAR) markers of Helicoverpa armigera: a new polymerase chain reaction‐based technique for predator gut analysis

Agustí¹,

Vicente²,

Gabarra³

1999

Molecular Ecology

107

104

View full text Add to dashboard Cite

show abstract

“…Cultivar identification and phylogeny A large set of markers has been produced to characterise grape cultivars, with particular reference to RFLPs (Bourquin et al 1993), AFLPs (Cervera et al 1998, RAPDs (Stravrakakis and Biniari 1998), SCARs (Xu and Bakalinski 1996), and microsatellites (Botta et al 1995;Bowers et al 1996Bowers et al , 1999aCipriani et al 1994;Lamboy and Alpha 1998;Sefc et al 1997Sefc et al , 1999Thomas and Scott 1993;Thomas et al 1994). The origin of the classic European wine grapes has been the subject of much speculation and the parental relationships were analysed by means of microsatellite loci in more than 300 cultivars (Bowers and Meredith 1997;Bowers et al 1999b).…”

Section: Grapementioning

confidence: 99%

Genetic modification of agronomic traits in fruit crops

Baldoni¹,

Rugini²

2002

Fruit and Vegetable Biotechnology

View full text Add to dashboard Cite

“…Walker and Liu [24] by using 5 isoenzyme systems were able to obtain a unique isoenzyme profile for each rootstock. Research for easier and more reproducible markers went on with the assessment of RAPD markers [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Nuclear SSR-based Genetic Diversity and Structure Analysis of Vitis Rootstocks

Bibi

2022

ARTOAJ

View full text Add to dashboard Cite

We have genetically identified 43 plants representing 28 rootstocks from three different ampelographic collections in Greece with the use of seven nuclear single sequence repeats (SSR) previously characterized. Analysis of SSR alleles and a similarity dendrogram construction was followed by a STRUCTURE and a hierarchical STRUCTURE analysis. We also compared the obtained genetic data with literature data about the origins of these rootstocks. In most cases known pedigrees of the studied rootstocks were confirmed but in some other cases microsatellite profiling did not support the pedigrees data, casting a doubt about the identity of a particular rootstock or on pedigree given by the literature. The genetic profiling of these plants enabled to reassign wrong identities in the collections and proved to be very efficient in assessing rootstock identities in a commercial dispute. The identity of an unknown rootstock was also determined. This work provides the molecular fingerprinting of 28 rootstocks and adds to their ancestry characterization.

show abstract

Identification of Grape (Vitis) Rootstocks Using Sequence Characterized Amplified Region DNA Markers

Cited by 19 publications

References 5 publications

Development of sequence amplified characterized region (SCAR) markers of Helicoverpa armigera: a new polymerase chain reaction‐based technique for predator gut analysis

Development of sequence amplified characterized region (SCAR) markers of Helicoverpa armigera: a new polymerase chain reaction‐based technique for predator gut analysis

Genetic modification of agronomic traits in fruit crops

Nuclear SSR-based Genetic Diversity and Structure Analysis of Vitis Rootstocks

Contact Info

Product

Resources

About