Genetic modification of agronomic traits in fruit crops

Baldoni, Luciana; Rugini, E.

doi:10.1533/9781855736412.1.25

Cited by 3 publications

(1 citation statement)

References 449 publications

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“…Moreover, nearly all Crimson Seedless vineyards in warm regions require Ethrel (ethephon) in the véraison for optimum color development, while the application of GA at bloom reduces fruitset and increases berry length and berry weight but also reduces berry color, increases berry shatter, and decreases return fruitfulness (Dokoozlian and Peacock 2001;Avenant and Avenant 2006). The development of specific genetic transformation systems would allow the introduction of genes that confer resistance to biotic or abiotic stresses (Baldoni and Rugini 2002). Moreover, efficient genetic transformation systems are valuable tools for functional genomic studies, now that grape genome has been sequenced by the French-Italian Public Consortium for Grapevine Genome Characterization (Jaillon et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Development of highly efficient genetic transformation protocols for table grape Sugraone and Crimson Seedless at low Agrobacterium density

López-Pérez

Velasco

Pazos-Navarro

et al. 2008

Plant Cell Tiss Organ Cult

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Highly efficient genetic transformation protocols and the regeneration of transgenic plants of Sugraone and Crimson Seedless grapevines (Vitis vinifera L.) were achieved from embryogenic calli co-cultured with low Agrobacterium tumefaciens densities. The sensitivity of embryogenic cultures to kanamycin, as well as the effect of Agrobacterium strains, C58(pMP90) or EHA105, and the bacterial concentration (0.06 or 0.2 at Optical Density OD 600 ) on transformation efficiency were studied. Embryogenic cultures showed different kanamycin sensitivities and the total suppression of embryo differentiation at 20 and 50 mg/l kanamycin for Crimson Seedless and Sugraone, respectively. sgfp gene expression was evaluated in callus co-cultured with each bacterial strain. Although GFP transient expression was higher with A. tumefaciens EHA105 in both cultivars at the beginning of the culture, there were no significant differences 28 days post-inoculation. However, the concentration of Agrobacterium did affected transformation efficiency: 0.06 OD 600 being more effective for the transformation of Crimson Seedless and 0.2 OD 600 for Sugraone. By following the optimised procedure, 21 and 26 independent transgenic plants were generated from Sugraone and Crimson Seedless respectively, three to five months post-infection. PCR analyses were carried out to verify the integration of the sgfp and nptII genes into grapevine genome and the stable integration of the sgfp gene was confirmed by Southern blot.

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Section: Introductionmentioning

confidence: 99%

Development of highly efficient genetic transformation protocols for table grape Sugraone and Crimson Seedless at low Agrobacterium density

López-Pérez

Velasco

Pazos-Navarro

et al. 2008

Plant Cell Tiss Organ Cult

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Agrobacterium-mediated transformation of Persian walnut usingBADHgene for salt and drought tolerance

Bolagh

Solouki

Tohidfar

et al. 2020

The Journal of Horticultural Science and Biotechnology

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Recursos genéticos y mejoramiento de frutales andinos: una visión conceptual

Arias

2007

CTA

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Andean fruits comprise species at different development stages, and of potential importance for their countries. Their cultivation is generally with heterogeneous landraces without technology. Higher efficiency and competitiveness of these fruits depends on the development of germplasm collections of cultivated taxa and related species, leading to varieties which overcome limiting problems. On the short term, the cultivar offer could be based on selection processes of local populations with a participative approach and cloning of outstanding individuals. In the medium and long term, the creation of a wide genetic base, enriched with attributes of the wild relative species is key. With this, selection processes and massive cloning using tissue culture to propagate different clones to avoid vulnerability. To date in Colombia, several collections of Andean fruits have been assembled and genetic variability characterized, and some breeding activities have been achieved. These are lulo domestication, prebreeding and breeding, and tomato tree preebreeding, based on interespecific hybridization to transfer fruit anthracnose resistance. This experience allows the development of a proposal on genetic resource-production of improved materials as an effective method to support productive function based on genetics.Recursos genéticos y mejoramiento de frutales andinos: una visión conceptual R E S U M E N Los frutales andinos comprenden especies con diversos grados de desarrollo y con potencial importante en los países del área. Su cultivo generalmente se realiza con materiales de agricultor heterogéneos sin aplicación de recomendaciones tecnológicas con enfoque sistémico. La eficiencia productiva y la competitividad de estas especies dependen de la constitución de colecciones de los taxa cultivados y especies relacionadas, debidamente conocidos en sus atributos, que hagan posible el desarrollo de variedades que representen soluciones a problemas limitantes. En el corto plazo la oferta de materiales para la siembra puede basarse en procesos selectivos en las poblaciones locales, con enfoque participativo y clonación de individuos superiores. En el mediano y largo plazos ésta puede enfocarse en la creación de una base genética amplia, enriquecida con atributos de las especies silvestres relacionadas. La selección y clonación masiva deben apoyarse, preferiblemente, en cultivo de tejidos, con propagación de diversos clones para prevenir la vulnerabilidad. Hasta el presente, en Colombia se han conformado colecciones de varios frutales andinos, se han llevado a cabo procesos de caracterización de la variabilidad y se han desarrollado algunas actividades de mejoramiento. éstas corresponden a domesticación, premejoramiento y mejoramiento en lulo y premejoramiento en tomate de árbol con relación a la incorporación de resistencia a la antracnosis de los frutos. Las experiencias sirven para proponer el desarrollo de una plataforma recursos genéticos/oferta de materiales mejorados, que apoye eficazmente la función productiva desde la óp...

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Genetic modification of agronomic traits in fruit crops

Cited by 3 publications

References 449 publications

Development of highly efficient genetic transformation protocols for table grape Sugraone and Crimson Seedless at low Agrobacterium density

Development of highly efficient genetic transformation protocols for table grape Sugraone and Crimson Seedless at low Agrobacterium density

Agrobacterium-mediated transformation of Persian walnut usingBADHgene for salt and drought tolerance

Recursos genéticos y mejoramiento de frutales andinos: una visión conceptual

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