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The aim of the present study was to determine the efficacy of the application of mixtures containing various combinations of humic substances, with herbicides and nitrogen fertilizers, in weed control and optimizing the plant nutrition system. We also aimed to evaluate the influence of these substances on winter wheat productivity. Five Ukrainian winter wheat cultivars (‘Kryzhynka,’ ‘Smuhlyanka,’ ‘Slavna,’ ‘Kubus,’ and ‘Mulan’) were sown in a randomized complete block design, with three replications, in the years 2014–2019. The analysis of the effect of the compositions containing herbicides, with various physiologically active substances, in a mixture with humic preparations (Humifield, 4R Foliar concentrate) was performed by counting weeds per square meter in each experimental plot. The best performance in weed control, including perennial species, was obtained from using a mixture of Grodil Maxi herbicide with the humic preparation, Humifield. The crop treatment of this mixture resulted in a 23.6% reduction in weeds, compared to the treatment with the Grodil Maxi herbicide only. At the same time, the complex application of a number of herbicides in a mixture with the humic preparation, 4R Foliar concentrate led to the opposite effect. Various applications of mixtures of humates (4R Foliar concentrate, 5R SoilBoost) with nitrogen fertilizers (ammonium nitrate; carbamide-ammonium mixture) to optimize the winter wheat nutritional system and yield increases have been studied. The highest yield increase of 20%–22% was harvested in the plots treated with 5R SoilBoost and 4R Foliar concentrate plus ammonium nitrate. In addition, the efficacy of wheat crop foliar feeding with mixtures of humates, plus a carbamide-ammonia mixture, in different phases of vegetation has been established. A yield increase of 10.0%–21.4% resulting from the use of such compositions was obtained.

Flow cytometry (FCM) analysis of live antibody-coated spermatozoa subjected to immunofluorescence staining (FCM test) is considered an objective method for the quantitative detection of antisperm antibodies (ASA). But the cross-linking of cell surface antigen (Ag) with bivalent antibodies and/or antigen-antibody (Ag-Ab) complexes with second antibodies may induce the reorganization of surface components (patching and capping) and result in their shedding from the sperm surface. The present study estimates the relationship between aggregation of Ag-Ab complexes on the sperm surface and the results of indirect FCM test. Swim-up spermatozoa of normozoospermic men were incubated with ASA-positive sera from infertile patients and with second antibodies fluorescein isothiocyanate (FITC)-labelled goat anti-human IgG polyclonal antiserum under different conditions and then analysed by FCM and fluorescence microscopy. It was shown that low temperature, cytochalasin B, excess or lack of the primary and/or secondary antibodies and sperm fixation by paraformaldehyde may inhibit aggregation and shedding of Ag-Ab complexes and dramatically increase ASA quantity determined on the sperm surface. However, inhibition of aggregation on the live sperm surface was observed only in a minority of ASA-positive samples and was poorly reproducible using semen of different donors. A high probability of Ag-Ab complex shedding from the sperm surface during experimental manipulation limits the use of indirect FCM test for quantitative ASA determination.

<p>An investigation of the effects of UV-C radiation from source having the power of 20 W on improvement of seed quality of Ukrainian winter wheat (<em>Triticum aestivum </em>L.) six cultivars: ‘Gratsіia Myronivska’, ‘Trudivnytsia Myronivska’, ‘Vezha Myronivska’, ‘Mudrist Odeska’, ‘Nyva Odeska’ and ‘Lira Odeska’, has been done. In the performed experiment the UV-C irradiation in the range of 50-1000 J m^-2 was applied. Based on the experimental results it is concluded that irradiation of UV-C on wheat seeds stimulated the seed vigour and germination. It has been established the optimal irradiation dose of UV-C radiation - 250 J m^-2 for ‘Trudіvnitsia Myronіvska’, ‘Mudrіst Odeska’, ‘Nyva Odeska’, ‘Lіra Odeska’ cultivars and 500 J m^-2 for ‘Hratsіia Myronіvska’ and ‘Vezha Myronіvska’ cultivars, which leads to maximum effect. The highest biometric indices of seedling structural elements were obtained from the seeds of all wheat cultivars, for which the values of seed vigour and germination were also maximum ones, i.e. at the same irradiation dose.</p>

Photodynamic therapy (PDT) is a method of the treatment of localized cancers, based on a photochemical reaction between a light-activated molecule or photosensitizer (PS), light, and molecular oxygen. Correct choice of PS is of fundamental importance for PDT efficacy. Despite numerous studies in this field, most known PS have some drawbacks, e.g. lack of specificity and aggregation in aqueous media. Consequently, the search for an ideal PS is essential for further development of PDT. Here we review classification and analyse main features of different generations of PS and describe the mechanisms of their action. Various methods of targeted delivery of PS to tumor cells are discussed. The advantages of PS nanoparticles with the effect of aggregation-induced emission (AIE) over the classic photosensitizers are presented. A possibility of practical application of such light-emitting structures in cancer phototherapy is shown. K e y w o r d s: photodynamic therapy (PDT), photosensitizer, aggregation-induced emission ABBrEVIATIONS AEMA -2-aminoethyl methacrylate ALA -5-aminolevulinic acid AlPc -aluminum-phthalocyanine chloride AlPcS4 -aluminium-phthalocyanine tetrasulphonated (Photosens) AlS2Pc -disulfonated phthalocyanine BDP -borondipyrromethene BHQ3 -black hole quencher3 BSA -bovine serum albumin c(rGDfc) -Arg-Gly-Asp-d-Phe-Cys cyclicpeptide crGD -cyclic arginine-glycine-aspartic acid DCF-DA -dichlorofluoresceindiacetate DMSO -Dimethylsulfoxide DPBA-TPE -(3,3'-(2,5-dimethoxy-1,4-phenylene)bis(2-(4bromophenyl)acrylonitrile)-tetraphenylethene DSPE-PEG-Mal -1,2-distearoyl-sn-glycero3-phosphoethanolamine-N-[maleimide(polyethylene glycol)] DTPEBBTD -Donor-tetraphenylethene-benzo[1,2-c:4,5-c']bis([1,2,5]thiadiazole) Fr/NIr -far-red/ near-infrared GFLG -Gly-Phe-Leu-Gly-peptide HPMA -N-(2-hydroxypropyl)methacrylamide m-THPP -meta-tetra(hydroxyphenyl)porphyrin (Foscan)

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Aim. To study the impact of complex preparations, containing humic, fulvic and ulmic acids in combination with herbicides and nitrogen fertilizers, on winter wheat yield. Methods. Field, laboratory, statistical methods. Results. The impact of herbicides with different active substances and their mixtures with humic preparations Humifi eld and Foliar concentrate on weed infestation and productivity of Kryzhynka winter variety was investigated. It was determined that the application of herbicide Prima (400 g/ha) and the mixture of preparations Triger (25 g/ha) + Tomigan (0.5 l/ha) in combination with humic preparation Humifi eld in the dose of 200 g/ha had practically no impact on the crop productivity. A considerable increase in the yield, for instance, by 15.6–20.3 %, was observed in case of spraying the fi elds with the same preparation forms of herbicides in the tank mixture with humic stimulator 4R Foliar concentrate in the dose of 2.0 kg/ha. The application of humates in combination with nitrogen fertilizers with the purpose of optimizing the nutrition system for winter wheat via their introduction superfi cially and by spraying the leaf-stem mass of plants was studied. It was demonstrated that the application of the growth regulator 5R SoilBoost in the amount of 11 kg/ha in the mixture with 200 kg/ha of ammonia nitrate led to the increase of productivity for Smuhlianka and Slavna varieties by 11.2 and 8.5 % respectively, and double foliar application of 4R Foliar concentrate (2+2 kg/ha) in the mixture with ammonia nitrate – by 15.5 %. The maximal increase in productivity by 20–23 % was obtained after combined application of humic stimulators 5R SoilBoost (11 kg/ha) and 4R Foliar concentrate (2+2 kg/ha) on the background of ammonia nitrate (200 kg/ha of physical weight). The effi ciency of foliar fertilization for wheat fi elds of Kubus and Mulan varieties using the mixtures of humates and carbamide-ammonia mixture in different phases of crop development was analyzed. The application of such combinations also promoted the productivity increase by 10.0–21.4 %. Conclusions. The increase in productivity of Kryzhynka winter wheat variety by 0.64–0.84 t/ha was determined after spraying crop fi elds with the tank mixture of herbicides and humic stimulator 4R Foliar concentrate in the dose of 2.0 kg/ha. The effi ciency of optimizing the nutrition system of plants via separate or combined application of humic preparations, in particular, granulated 5R SoilBoost (11 kg/ha), superfi cially, and 4R Foliar concentrate (2 kg/ha+2 kg/ ha) in case of foliar fertilization for fi elds in different phases of crop development on the background of early spring introduction of ammonia nitrate (200 kg/ha) to frozen-thawed soil was proven. The increase in wheat productivity was observed in all variants of applying these mixtures. However, the maximal increase in the winter wheat yield was obtained due to the fertilization technology, envisaging the use of humates 5R SoilBoost and 4R Foliar concentrate on the background of ammonia nitrate. There was a noted increase in grain productivity of winter wheat varieties Kubus and Mulan by 0.50–0.94 and 0.41–1.08 t/ha respectively in case of superfi cial introduction of humic preparation 5R SoilBoost (11 kg/ha) and foliar fertilization of wheat fi elds with 4R Foliar concentrate (2+2 kg/ha) in combination with carbamide-ammonia mixture (200 + 100 kg/ha).