The influence of a medicinal plant extract, immobilised by ligands, on the activity of antioxidant enzymes and photosynthetic pigment concentration of wheat and maize was studied. The object of study was seed of drought-resistant firm durum wheat (Triticum durum Desf.) and maize (Zea mays L.). Seeds were subjected to general uniform γ-radiation from a 60Co source on a Rkhund installation at average dose power of MD = 0.306 Gy/sec. Before radiation seeds were treated in modified extract from medicinal plants. The treatment of seeds with 0.1 and 0.01% solution of modified extract from Hypericum, Dandelion, and Calendula caused significant reduction in processes initiated by radiation and in formation of free radicals. On the basis of the obtained results it was concluded that the used modified plant extract collection had a protective effect, reducing the amount of free radicals produced by γ-irradiation.
Aim. In order to study the radioprotective activity were were obtained in the tryptophanates of cobalt (II), manganese (II), copper (II) and zinc. Methods. The composition and structure of the complexes were studied by elemental, thermogravimetric analyzes and IR infrared spectroscopy. To determine the presence of the Radioprotective Properties of the Co (II), Mn (II), Cu (II), Zn complexes with tryptophane, a test was conducted for the 30-day survival of irradiated animals. Results. The results of thermogravimetric studies have shown that the final product of the thermal decomposition of all compounds is metal oxide, respectively. The method of IR spectroscopy showed that the ligands in the composition of the metal (II) complexes enter the neutral form and coordinate with the complexing agent through the nitrogen atom. The results of experiments with complexes showed that they have noticeable radioprotective activity. The radioprotective activity of Co (II) complexes with tryptophan is 55%, Mn (II) with tryptophan 50%, Cu (II) with tryptophan 40%, and Zn 30%. They improve the survival and average life expectancy of lethally irradiated mice, not reaching the level of the known cystamine radioprotector, which is 80%. Conclusions. The data obtained by us testify to the prospects of using the complexes of Co (II), Mn (II), Cu (II), Zn with tryptophan for preventive purposes and in order to prevent local radiation injuries. Keywords: tryptophan-metal complexes, IR spectroscopy, thermogravimetry, complex compounds, radioprotective properties.
Aim. The synthesized and characterized of the complex nickel (II) with tryptophan [NiL2 (H2O)2]; L = Trp. It is shown that the composition of the complex obtained depends on the ratio of the initial components. Methods. The composition and structure of the complex were studied by elemental, TG/DrTG-DTA analysis and IR spectroscopy. The method of IR showed that the ligand in the composition of the metal complex enter the neutral form and coordinate with the complexing agent through the nitrogen atom. Results. The results of thermogravimetric studies have shown that the final product of the thermal decomposition of all compound is metal oxide, respectively. Conclusions. Have been studied the biological properties – the toxicity has been evaluated, the influence of the complex on the resistance to hypoxia and ant radiation activity has been determined. Keywords: tryptophan, nickel, tryptophan-metal complexes, amine groups, IR spectroscopy, thermogravimetry, toxicity, antiradiation activity.
Researches spent in the field of biocoordination chemistry are based on the use of the model biologically active molecules containing functional groups, characteristic for biological systems. It is known, that vital metals are mainly in the form of coordination connections in an organism. During influence of various stressful factors, including ionizing radiation permeability of cellular membrane changes, which leads to dysfunction of metalloenzymes. Therefore exogenous complexes are in a sense irreplaceable additional sources maintenance of organisms (including plant) with microelements -metals. There are significant amount of works, testifying interaction of complexes of metals with free radicals. It is shown, that complexes serve as traps of the free radicals, collecting in an organism, transforming them into non radical products. Presence of organic ligand gives lipophily to metallocomplexes and as a result their transport through the cellular membrane is strongly facilitated. Reasoning from the above-stated, creation of new biologically active preparations on the basis of coordination connections of the metals containing various organic ligands, including natural, is actual. Use of iron as a radioprotector is connected with its ability to complexing. The task of our researches was studying action of gamma irradiation on biosynthesis of chlorophylls and carotinoids, on an output of chromosomal aberrations at anaphase cells of root fibrils of wheat sprouts at use of complexes of iron with the purpose of revealing their radioprotective properties. As the object of researches were taken seeds of a drought-resistant sort of hard wheat Triticum durum L. Seeds were subjected to general proportional gamma irradiation from a source 60Co on gamma-installation "Ê-25" at the doze of 47,83 rad/sec. Before an irradiation seeds had been processed with solutions of pirocatechine, pirocatechinate of iron, thiocarbamide, thiocarbamide of iron, rutin and rutinate of iron in concentration of 10-2 M. Among the used complexes the most effective were pirocatechinate of iron, rutinate of iron which promoted significant reduction of chromosomal aberrations. It is necessary to note, that radioprotective ability of pirocatechinate of iron and rutinate of iron is also found out at processing seeds after an irradiation. It is revealed, that pirocatechinate and rutinate iron possess more expressed radioprotective properties and processing of seeds of hard wheat by solutions of these complexes stimulate formation of photosynthetic apparatus and considerably reduce number of chromosomal aberrations at an irradiation.
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