Filamentous fungi in 36 samples of Antarctic permafrost sediments were studied. The samples collected during the Russian Antarctic expedition of 2007-2009 within the framework of the Antarctic Permafrost Age Project (ANTPAGE) were recovered from different depths in ice-free oases located along the perimeter of the continent. Fungal diversity was determined by conventional microbiological techniques combined with a culture-independent method based on the analysis of internal transcribed spacer (ITS2) sequences in total DNA of the samples. The study revealed a rather low fungal population density in permafrost, although the diversity found was appreciable, representing more than 26 genera. Comparison of the data obtained by different techniques showed that the culture-independent method enabled the detection of ascomycetous and basidiomycetous fungi not found by culturing. The molecular method failed to detect members of the genera Penicillium and Cladosporium that possess small-sized spores known to have a high resistance to environmental changes.

Introduction. In patients with chronic loco-motor system diseases the pain often persists after arthroplasty and does not respond to symptomatic therapy.Purpose of study: to evaluate the efficacy of perioperative use of Meloxicam in patients after hip arthroplasty. Patients and methods. The follow-up covered 120 patients (mean age 64.4±5.23 years) after hip arthroplasty. In the main group (n=60) Meloxicam was given 7 days prior to and 3 weeks after surgery; after intervention narcotic analgesic was used on the request. In control group (n=60) the patients were only on narcotic analgesic on request after operation. Treatment results were assessed by 100-millimeter visual analog scale (VAS) and D’Aubigné-Postel Score.Results. Seven days before the surgery the pain severity in the main and control groups was comparable: 85±2.3 and 84±2.1, respectively. In 2 days after operation the pain relief was more pronounced in the main group — 69±2.1 mm versus 82±3.4 mm in the control group (p<0.05). In 3 months those indices made up 10±2.1 and 35±12.6 mm (p<0.001), respectively. In the main group the result was assessed as the excellent and good in 22 patients, in the rest of patients as satisfactory by d’Aubigné-Postel Score. In the control group the good result was recorded in 9, satisfactory — in 47 and poor — in 4 cases.Conclusion. Meloxicam may be considered as an effective perioperative analgesic in large joints arthroplasty.

Analysis of safety and efficacy of the currently marked peroral anticoagulants was performed in long term follow up of patients after large joints arthroplasty. Total number of 5025 patients after total knee arthroplasty and 5216 patients after total hip arthroplasty were examined. All patients were divided into groups depending on the prescribed anticoagulant. The course of anticoagulant therapy made up 35 days and 6 weeks after surgery, respectively. US examination of the veins of lower extremities was performed to all patients prior to and on day 5 after operation. In patients on Dabigatran the rate of clinically significant deep vein thrombosis was lower than in group of patients on Rivaroxaban and Apixaban, but the rate of postoperative hematomas in lust two groops was higher.

The article has emphasized the problem of injuries related to alcohol consumption. An analysis of contemporary research on this topic and own data have been given. Intoxicated patients had more severe injuries, a more serious course of illness, a greater incidence of complications and mortality. Alcohol intoxication provoked formation of delayed intracranial hematomas. The tendency to form multiple intracranial hematomas and subarachnoid hemorrhages in alcohol abusers was explained by atrophic processes in the brain, subdural spaces extension, toxic lesions of the cerebral vessels. Intoxicated patients should be regarded as a high-risk group in terms of availability of severe traumatic brain injuries, as their condition

No abstract

Существует необходимость разработки алгоритмов лечебной и восстановительной тактики при травме на фоне алкоголизации. Алкогольное потребление создает ряд дополнительных повреждающих факторов, связанных с метаболическими и стрессогенными эффектами этанола. Основной задачей интенсивной терапии становится нормализация нарушений, характеризующих состояние гомеостаза и энергетического обмена, и восстановление нарушенных функций и систем. Это коррекция нарушений микроциркуляции и эндотелиальной дисфункции, нейропротекция с помощью субстратных антигипоксантов, препаратов лития, обладающих антиапоптозным действием, заместительная иммунокоррекция и купирование гипоальбуминемии. При травме в сочетании с алкоголизмом для коррекции энергетического обмена использование аминокислот как энергетического субстрата более предпочтительно, так как они напрямую включаются в цикл трикарбоновых кислот и их биохимические пути превращения -короткие и энергетически наиболее выгодные, т.е. в экстремальных условиях для организма оптимально использовать аминокислоты, а не углеводы и липиды. Таким образом, терапевтическая тактика при травме, осложненной алкоголизмом, вызывает необходимость учета и купирования дополнительных повреждающих факторов. Ключевые слова: сочетанная черепно-мозговая и скелетная травма, ассоциированная с алкогольным потреблением, интенсивная терапия травматической болезни на фоне алкоголизации. There is a need to develop algorithms for medical and rehabilitation tactics trauma on the background of alcohol abuse. Alcohol consumption poses a number of other damaging factors related to the metabolic effects of ethanol and stressful. The main objective of intensive therapy becomes normalization of disorders characterized by a state of homeostasis and energy metabolism, and the restoration of the disturbed functions and systems. This correction of microcirculation and endothelial dysfunction, neuroprotection via substrate antihypoxants, lithium drugs with anti-apoptotic effect, replacement immunotherapy and relief hypoalbuminemia. When trauma in combination with the use of alcohol for correcting amino acids as an energy substrate and more preferably the energy metabolism, since they directly enter the TCA and their biochemical conversion path -short and the energetically most favorable, i.e. in extreme conditions optimal for the organism is-polzovat amino acids rather than carbohydrates and lipids. Thus, the therapeutic tactics in trauma complicated by alcoholism, is the need to integrate relief and other damaging factors. Keywords: combined craniocerebral and skeletal injury associated with alcohol consumption, intensive therapy of traumatic disease on the background of alcohol abuse. SIGNIFICANT PATHOGENETIC THERAPY IN TRAUMATIC DISEASES ASSOCIATED

Analysis of the safety and efficacy of the currently marked oral anticoagulants in patients after large joints arthroplastyat outpatient step was presented for the first time in Russian clinical practice. Total number of 5025 patients after total knee and 5216 patients after total hip arthroplasty were examined. All patients were divided into 3 groups depending on prescribed anticoagulant (dabigatran, rivaroxaban or apixaban). Duration of therapy with corresponding drug after hip and knee arthroplasty was initiated after surgery and made up 35 and 45 days, respectively. Deep vein US examination was performed to all patients in 3 and 6 months after discharge. In patients on dabigatran the rate of clinically significant deep vein thrombosis development was lower than in patients on rivaroxaban and apixaban, and made up 5% vs 7.7 and 16%, respectively. Rate of nonfatal pulmonary embolism (PE) was comparable. Relapse of deep vein embolism development was diagnosed only in patients who received rivaroxaban. A suggestion on the expediency of a longer thromboprophylaxis after surgical interventions on large joints was made that would require extensive studies.

Analysis of the safety and efficacy of the currently marked oral anticoagulants in patients after large joints arthroplastyat outpatient step was presented for the first time in Russian clinical practice. Total number of 5025 patients after total knee and 5216 patients after total hip arthroplasty were examined. All patients were divided into 3 groups depending on prescribed anticoagulant (dabigatran, rivaroxaban or apixaban). Duration of therapy with corresponding drug after hip and knee arthroplasty was initiated after surgery and made up 35 and 45 days, respectively. Deep vein US examination was performed to all patients in 3 and 6 months after discharge. In patients on dabigatran the rate of clinically significant deep vein thrombosis development was lower than in patients on rivaroxaban and apixaban, and made up 5% vs 7.7 and 16%, respectively. Rate of nonfatal pulmonary embolism (PE) was comparable. Relapse of deep vein embolism development was diagnosed only in patients who received rivaroxaban. A suggestion on the expediency of a longer thromboprophylaxis after surgical interventions on large joints was made that would require extensive studies.