Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cytofluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).
It is shown that normalizing the thickness of coatings in the design of products and finding the actual value of the thickness of the coating layer with a given accuracy in manufacturing is a pressing task. An analysis of the regulatory documentation was carried out, which showed that there are recommendations for normalizing the thickness of the coating to select only the minimum thickness of the coating. The maximum thickness of the coatings is not regulated, which leads to unnecessary over expenditure of the coating material, energy and other costs and does not allow selecting the means of measuring the thickness of the coatings depending on accuracy.
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