Cell-to-cell interactions in changed gravity: Ground-based and flight experiments

Буравкова, Л. Б.; Romanov, Yu. A.; Рыкова, М. П.; Grigorieva, Olga; Мерзликина, Н. В.

doi:10.1016/j.actaastro.2005.03.012

Cited by 51 publications

(31 citation statements)

References 17 publications

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“…Specifically, one study determined that a 7–day incubation in a 3D– с linostat caused an increase of the population ratio of human MMSC cells expressing stromal cell antigens CD44+, CD90+, CD29+ [39]. Another study showed that a 6–day incubation in a horizontal с linostat decreased the number of cells bearing the CD105 and HLA A,B,C antigens in a culture of human bone marrow MMSC [45]. Our own studies show that a 5–day incubation of MMSC on a RPM causes an increase in the number of cells expressing integrin CD49b, but does not affect the percentage of cells, expressing CD29 [46].…”

Section: The Role Of Adhesion Receptors In Regulating Precursor Cell mentioning

confidence: 99%

Mechanisms of Gravitational Sensitivity of Osteogenic Precursor Cells

Буравкова

Gershovich

et al. 2010

Acta Naturae

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This report is a detailed review of the current data on the mechanic and gravitational sensitivity of osteoblasts and osteogenic precursor cells in vitro. It summarizes the numerous responses of cells with an osteoblastic phenotype and osteogenic precursor cells and especially their responses to the alteration of their mechanic or gravitational surroundings. The review also discusses the osteogenic cell’s pathways of signal transduction and the mechanisms of gravitational sensitivity. It was shown that the earliest multipotent stromal precursor cells of an adult organism’s bone marrow can sense changes of intensity in a gravitational or mechanic field in model conditions, which may play a certain role in the development of osteopenia in microgravity.

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Section: The Role Of Adhesion Receptors In Regulating Precursor Cell mentioning

confidence: 99%

Mechanisms of Gravitational Sensitivity of Osteogenic Precursor Cells

Буравкова

Gershovich

et al. 2010

Acta Naturae

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“…11,13,17 Endothelial cells are one type of cells which are affected by weightlessness. 22,23 They form the endothelium, which is located at the interface between the blood and the vessel wall. The endothelium of all blood vessels is highly specialized with endocrine, exocrine, cell adhesion, clotting and transport functions.…”

Section: Introductionmentioning

confidence: 99%

Induction of three-dimensional assembly and increase in apoptosis of human endothelial cells by simulated microgravity: Impact of vascular endothelial growth factor

et al. 2006

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Endothelial cells play a crucial role in the pathogenesis of many diseases and are highly sensitive to low gravity conditions. Using a three-dimensional random positioning machine (clinostat) we investigated effects of simulated weightlessness on the human EA.hy926 cell line (4, 12, 24, 48 and 72 h) and addressed the impact of exposure to VEGF (10 ng/ml). Simulated microgravity resulted in an increase in extracellular matrix proteins (ECMP) and altered cytoskeletal components such as microtubules (alpha-tubulin) and intermediate filaments (cytokeratin). Within the initial 4 h, both simulated microgravity and VEGF, alone, enhanced the expression of ECMP (collagen type I, fibronectin, osteopontin, laminin) and flk-1 protein. Synergistic effects between microgravity and VEGF were not seen. After 12 h, microgravity further enhanced all proteins mentioned above. Moreover, clinorotated endothelial cells showed morphological and biochemical signs of apoptosis after 4 h, which were further increased after 72 h. VEGF significantly attenuated apoptosis as demonstrated by DAPI staining, TUNEL flow cytometry and electron microscopy. Caspase-3, Bax, Fas, and 85-kDa apoptosis-related cleavage fragments were clearly reduced by VEGF. After 72 h, most surviving endothelial cells had assembled to three-dimensional tubular structures. Simulated weightlessness induced apoptosis and increased the amount of ECMP. VEGF develops a cell-protective influence on endothelial cells exposed to simulated microgravity.

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“…The expression of various surface adhesion factors was changed, but despite these effects, no critical physiological processes were compromised. 95 Zhang et al reported earlier that mesenchymal stem cells showed an increased endothelial differentiation potential when cultured on a clinostat for 72 h. 96 Further, clinorotation also has some detrimental consequences for ECs. Kang et al observed that prolonged growth on a clinostat induced among others apoptosis in microvascular ECs.…”

Section: 83mentioning

confidence: 99%

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

Grimm

Wehland

Pietsch

et al. 2014

Tissue Engineering Part B: Reviews

121

137

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Tissue engineering in simulated (s-) and real microgravity (r-mg) is currently a topic in Space medicine contributing to biomedical sciences and their applications on Earth. The principal aim of this review is to highlight the advances and accomplishments in the field of tissue engineering that could be achieved by culturing cells in Space or by devices created to simulate microgravity on Earth. Understanding the biology of three-dimensional (3D) multicellular structures is very important for a more complete appreciation of in vivo tissue function and advancing in vitro tissue engineering efforts. Various cells exposed to r-mg in Space or to s-mg created by a random positioning machine, a 2D-clinostat, or a rotating wall vessel bioreactor grew in the form of 3D tissues. Hence, these methods represent a new strategy for tissue engineering of a variety of tissues, such as regenerated cartilage, artificial vessel constructs, and other organ tissues as well as multicellular cancer spheroids. These aggregates are used to study molecular mechanisms involved in angiogenesis, cancer development, and biology and for pharmacological testing of, for example, chemotherapeutic drugs or inhibitors of neoangiogenesis. Moreover, they are useful for studying multicellular responses in toxicology and radiation biology, or for performing coculture experiments. The future will show whether these tissue-engineered constructs can be used for medical transplantations. Unveiling the mechanisms of microgravity-dependent molecular and cellular changes is an up-to-date requirement for improving Space medicine and developing new treatment strategies that can be translated to in vivo models while reducing the use of laboratory animals.

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Cell-to-cell interactions in changed gravity: Ground-based and flight experiments

Cited by 51 publications

References 17 publications

Mechanisms of Gravitational Sensitivity of Osteogenic Precursor Cells

Mechanisms of Gravitational Sensitivity of Osteogenic Precursor Cells

Induction of three-dimensional assembly and increase in apoptosis of human endothelial cells by simulated microgravity: Impact of vascular endothelial growth factor

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

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