Mesenchymal Stem Cells from Human Bone Marrow and Adipose Tissue: Isolation, Characterization, and Differentiation Potentialities

Romanov, Yu. A.; Darevskaya, A. N.; Мерзликина, Н. В.; Буравкова, Л. Б.

doi:10.1007/s10517-005-0430-z

Cited by 161 publications

(116 citation statements)

References 18 publications

(43 reference statements)

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“…As indicated in Table 1, mRNA expression of several of these protein markers was confirmed by real-time quantitative PCR analysis (data not shown). These findings are in agreement with reports made for adipose-derived stromal cells from humans, rodents, and non-human primates (Safford, Hicok et al 2002;Zuk, Zhu et al 2002;Ashjian, Elbarbary et al 2003;Safford, Safford et al 2004;Fujimura, Ogawa et al 2005;Romanov, Darevskaya et al 2005;Ning, Lin et al 2006). …”

Section: Morphological Differentiation and Expression Of Neuro-glial supporting

confidence: 93%

Autologous transplants of Adipose-Derived Adult Stromal (ADAS) cells afford dopaminergic neuroprotection in a model of Parkinson's disease

McCoy

Martinez

Ruhn

et al. 2008

Experimental Neurology

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Adult adipose contains stromal progenitor cells with neurogenic potential. However, the stability of neuronal phenotypes adopted by Adipose-Derived Adult Stromal (ADAS) cells and whether terminal neuronal differentiation is required for their consideration as alternatives in cell replacement strategies to treat neurological disorders is largely unknown. We investigated whether in vitro neural induction of ADAS cells determined their ability to neuroprotect or restore function in a lesioned dopaminergic pathway. In vitro-expanded naïve or differentiated ADAS cells were autologously transplanted into substantia nigra 1-week after an intrastriatal 6-hydroxydopamine injection. Neurochemical and behavioral measures demonstrated neuroprotective effects of both ADAS grafts against 6-hydroxydopamine-induced dopaminergic neuron death, suggesting that pre-transplantation differentiation of the cells does not determine their ability to survive or neuroprotect in vivo. Therefore, we investigated whether equivalent protection by naïve and neurally-induced ADAS grafts resulted from robust in situ differentiation of both graft types into dopaminergic fates. Immunohistological analyses revealed that ADAS cells did not adopt dopaminergic cell fates in situ, consistent with the limited ability of these cells to undergo terminal differentiation into electrically active neurons in vitro. Moreover, re-exposure of neurally-differentiated ADAS cells to serum-containing medium in vitro confirmed ADAS cell phenotypic instability (plasticity). Lastly, given that gene expression analyses of in vitro-expanded ADAS cells revealed that both naïve and differentiated ADAS cells express potent dopaminergic survival factors, ADAS transplants may have exerted neuroprotective effects by production of trophic factors at the lesion site. ADAS cells may be ideal for ex vivo gene transfer therapies in Parkinson's disease treatment.

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Section: Morphological Differentiation and Expression Of Neuro-glial supporting

confidence: 93%

Autologous transplants of Adipose-Derived Adult Stromal (ADAS) cells afford dopaminergic neuroprotection in a model of Parkinson's disease

McCoy

Martinez

Ruhn

et al. 2008

Experimental Neurology

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“…First, ASCs can be easily obtained from adipose tissues and maintained in stable growth and proliferation (42,44). Second, they can be differentiated into adipogenic, osteogenic, chondrogenic, neurogenic, or myogenic lineages (21,38,39,44). Third, ASCs secrete various cytokines such as interleukin-6 and growth factors such as basic fibroblast growth factor (bFGF), hepatocyte growth factor, and insulin-like growth factor (IGF), which are involved in muscle satellite cell activation and regeneration (7).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Mouse Adipose-Derived Stem Cells and Losartan in the Skeletal Muscle of Injured Mdx Mice

et al. 2015

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Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder caused by mutations in the dystrophin gene. Adipose-derived stem cells (ASCs) are an attractive source of cells for stem cell therapy. Losartan has been reported to improve ASC transplantation in injured mouse muscles. In the present study, we investigated whether the combined treatment of losartan and ASCs in the injured muscles of mdx mice improves regeneration. The combined treatment of ASCs and losartan remarkably improved muscle regeneration and induced muscle hypertrophy. In addition, ASCs and losartan treatment downregulated transforming growth factor-b and inhibited muscle fibrosis. We observed cells coexpressing green fluorescent protein (GFP) and dystrophin in the muscle samples of mice transplanted with GFP-positive ASCs. In the coculture in vitro experiment, we also observed that the GFP ASCs differentiated into dystrophin-expressing myotubes. The present study shows that the combination of transplanted ASCs and treatment with losartan ameliorated muscle fibrosis and improved muscle regeneration in injured mdx mice. Thus, we suggest that combined treatment with losartan and ASCs could help to improve muscle regeneration in the muscles of injured patients, including DMD patients.

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“…It was easy to separate the slurry of tissue into adipose and stromal cells during the process of centrifugation. As early as in the first stages of the study, it was shown that the capability of the cells to adhere was higher with this method than with enzyme incubation [17]. Researchers are still looking for more efficient methods of obtaining stem cells from the stromal fraction.…”

Section: Methods Of Isolating Adscsmentioning

confidence: 99%

“…Furthermore, they are positively stained for the presence of vimentin, ASMA, collagen (collagen-1) and fibronectin, but they do not include HLA-DR, CD117 or hematopoietic cell markers [17]. ADSCs are generally similar but not identical to BMSCs.…”

Section: The Phenotype Of Adscsmentioning

confidence: 99%

Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications

Harasymiak-Krzyżanowska

Niedojadło

Karwat

et al. 2013

Cellular and Molecular Biology Letters

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The stromal-vascular cell fraction (SVF) of adipose tissue can be an abundant source of both multipotent and pluripotent stem cells, known as adipose-derived stem cells or adipose tissue-derived stromal cells (ADSCs). The SVF also contains vascular cells, targeted progenitor cells, and preadipocytes. Stromal cells isolated from adipose tissue express common surface antigens, show the ability to adhere to plastic, and produce forms that resemble fibroblasts. They are characterized by a high proliferation potential and the ability to differentiate into cells of meso-, ecto- and endodermal origin. Although stem cells obtained from an adult organism have smaller capabilities for differentiation in comparison to embryonic and induced pluripotent stem cells (iPSs), the cost of obtaining them is significantly lower. The 40 years of research that mainly focused on the potential of bone marrow stem cells (BMSCs) revealed a number of negative factors: the painful sampling procedure, frequent complications, and small cell yield. The number of stem cells in adipose tissue is relatively large, and obtaining them is less invasive. Sampling through simple procedures such as liposuction performed under local anesthesia is less painful, ensuring patient comfort. The isolated cells are easily grown in culture, and they retain their properties over many passages. That is why adipose tissue has recently been treated as an attractive alternative source of stem cells. Essential aspects of ADSC biology and their use in regenerative medicine will be analyzed in this article.

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Mesenchymal Stem Cells from Human Bone Marrow and Adipose Tissue: Isolation, Characterization, and Differentiation Potentialities

Cited by 161 publications

References 18 publications

Autologous transplants of Adipose-Derived Adult Stromal (ADAS) cells afford dopaminergic neuroprotection in a model of Parkinson's disease

Autologous transplants of Adipose-Derived Adult Stromal (ADAS) cells afford dopaminergic neuroprotection in a model of Parkinson's disease

Therapeutic Effects of Mouse Adipose-Derived Stem Cells and Losartan in the Skeletal Muscle of Injured Mdx Mice

Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications

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