In recent years, interest in mountain savory is increasing and this is due to the rich biochemical composition, useful properties and decorative qualities of this plant. The materials were sortopopulyatsiya savory mountain. The study was conducted in the open ground in Moscow region (2012-2018) on experimental plots of the laboratory green, spicy taste and floral crops of FSBSI FSVC. The work on improving the source material by applying individual selection, the most aligned with economically valuable traits form ЧГ32-12 with high potential productivity of inflorescences and green mass, decorative, flavour and hardiness. Conducted testing and pre-multiplication of promising accessions, which was transferred into the "GOSSORTKOMISSIA" in 2018 called “Bobrik”. The yield of green increased in vegetation years in the first year of vegetation was 0,650 kg/m2, in the second – 3.0 kg/m2, and in the third year reached 5.4 kg/m2. Indicator sign "plant height" increased over the years of vegetation (11.4 cm, 25.5 cm and 40.2 cm, respectively). The values of the sign "number of shoots" in the first year of vegetation was 11.6 PCs., in the second year – sharply increased (255.1 PCs.), in the third year – reached its maximum (787.3). The accumulation of dry matter in the years of vegetation increased: the first year contained 20.08%; the second year – 26.95%; and the third year of vegetation – 29.71%. The content of ascorbic acid in the flowering phase in the second year of vegetation increased compared to the first year (8.21 mg/% and 5.87 mg (%). The amount of water-soluble antioxidants in the second year of the growing season has increased almost two times in comparison with the first year (to 22.7 mg/g and AAE 42.39 mg/g of AAE), in the third year there is a decrease in rate – 28.6 mg/g of AAE. Thus, the green of the mountain variety "Bobrik" is a source of biologically active substances, such as ascorbic acid and carotenoids, so the culture can be recommended for the use of young shoots and leaves in fresh and dry form, as a seasoning for salads, sauces, marinades, meat and fish dishes, as well as spicy and aromatic additives for the preparation of drinks.
In this work, we studied in vitro propagation of three cultivars of Mentha × piperita L. Murashige and Skoog medium (MS) supplemented with 0.5 mg·L−1 BAP was the most optimal medium for micropropagation of the cultivars studied. The ability of peppermint plants field-acclimated after in vitro micropropagation to produce essential oils (EOs) was investigated. EO was obtained by hydrodistillation from dried leaves and flowering shoots from control (field grown) plants and plants acclimated in field conditions after in vitro propagation. The samples were collected at the first and second year of vegetation, and their chemical composition was investigated using gas chromatography-mass spectrometry (GC-MS). Differences were observed in the yield, as well as in the quantitative and qualitative composition of the EOs extracted from the control plants and field-acclimated plants after in vitro propagation. Menthol was the main component of the EO in control plants, while pulegone and menthone were dominant in the EO pattern in field-acclimated in vitro regenerants in the first year of the growing season. However, in the second year of vegetation, the content of the main EO components in field-acclimated peppermint plants was approximately the same as in control plants. The antioxidant activity of EOs extracted from field-acclimated after in vitro micropropagation plants was found to be the same as in control field-grown M. × piperita plants.
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