On the model of Achilles tendon injury in rats, the antinociceptive and regenerative effect of allogeneic transplantation of various doses of adipose-derived mesenchymal stem cells (ADMSCs) into the lesion area was studied. It was found that netiher of tested regimens of allogeneic transplantation of ADMSCs contributed to a decrease in the edema of the injured limb. Among the studied regimens, only a twofold administration of 0.25 • 106 ADMSCs exhibited an antinociceptive effect. Administration of 0.50 • 106 ADMSCs promoted the accelerated onset of neovascularization of tendon tissue, while enhancing inflammation and the formation of granulation tissue. Double transplantation of 0.25 • 106 ADMSCs led to accelerated reorganization of collagen fibers, later neovascularization, however, there was an absence of inflammatory infiltrate, lipomatosis, and massive formation of granulation tissue in the lesion area.
Республика БеларусьЦель. Изучить ранозаживляющие свойства N-пальмитоилэтаноламина и N-стеароилэтаноламина в липосомальной форме, сравнить их действие с лекарственным средством с доказанной эффективностью.Материал и методы. Ранозаживляющее влияние N-пальмитоилэтаноламина и N-стеароилэтаноламина изучено на 214 белых рандомбредных крысах-самцах с тремя типами кожных ран: линейные, лоскутные и ожоговые. Для исследования каждого типа ран животные были разделены на группы: контрольная (раны не обрабатывались), отрицательный контроль (или растворитель), положительный контроль (мазь Метилу-рацил), пальмитоилэтаноламин и стеароилэтаноламин. Ежедневно проводили мониторинг общего состо-яния животных, фотосъемку ран. После этого поверхность ран обрабатывали исследуемыми веществами. Обработку ран продолжали до полного заживления с фиксированием суток заживления. Выводы делали на основании результатов визуального наблюдения, компьютерной планиметрии, данных морфологического исследования.Результаты. В случае применения обоих N-ацилэтаноламинов сокращение сроков заживления ли-нейных ран не зафиксировано, длительность периода заживления составила 8-10 суток. Лоскутные раны в контрольных группах заживали в течение 21-25 суток. В случае применения N-стеароилэтаноламина этот показатель составил 22-25 суток (p>0,05). Напротив, ежедневная аппликация пальмитоилэтаноламина на поверхность лоскутных ран приводила к их заживлению на 18-19 сутки (p<0,05). Термические ожоги IIIА степени в контрольных группах заживали в течение 30-32 суток. Применение обоих N-ацилэтаноламинов приводило к уменьшению этого срока до 27-28 суток, в случае N-стеароилэтаноламина эти изменения были достоверными (p<0,05).Заключение. N-пальмитоилэтаноламин и N-стеароилэтаноламин в липосомальной форме оказывают стимулирующее воздействие на регенерацию раневых дефектов кожи. Пальмитоилэтаноламин сокращает сроки заживления лоскутных кожных ран, N-стеароилэтаноламин ускоряет заживление ожоговых ран. N-ацилэтаноламины в липосомальной форме являются перспективными для использования в качестве ранозаживляющих средств. Дальнейшие исследования в этом направлении могут стать научной основой создания новых фармацевтических средств. Ключевые слова: ацилэтаноламин, липосомы, кожные раны, регенерация, эпидермис, дерма, ранозажив-ляющие свойства, создание новых фармацевтических средствObjectives. To study wound-healing properties of N-palmitoylethanolamine and N-stearoylethanolamine in liposomes and to compare their action with the drug has also proven to be an effective and safe.Methods. The wound-healing effects of N-palmitoylethanolamine and N-stearoylethanolamine were studied on white male rats (n=214) with three types of skin wounds: incision, excision and burn wounds. To study each type of wounds the animals were divided into groups: control (untreated wounds), negative control (or solvent), positive control (Methyluracil ointment), N-palmitoylethanolamine and N-stearoylethanolamine. The general state of animals was monitored daily; all wounds were photographed and then treat...
The adhesion and proliferation of rat adipose-derived mesenchymal stem cells (MSCs) as part of biocomposite with haemostatic drug Spongostan Absorbable Haemostatic Gelatin Powder as well as its regenerative efficiency after its introduction into the periurethral region in rats with experimental urinary incontinence were studied. It was found that MSCs rapidly adhere to the surface of the carrier and actively proliferate. The periurethral injection of biocomposite (Spongostan + MSCs) quickly and effectively recovers the urine continence function in animals that have undergone modeling of incontinence by urethrolysis. Spongostan hydrated in the ratio of 1 :24 inphosphate-saline buffer has a volume-forming effect in the urethra of experimental animals.
The derivatives of fatty acids are considered as promising bases for the development of medicinal and cosmetic agents. In this regard, it is necessary to study their potential toxicity, including chronic. In repeated toxicity experiments on mice of C57BL6 line, the administration of N-palmitoyl-5-aminolevulinic acid (within liposomes) for 62 days was less toxic than N-palmitoylglycine injected in the same regimen, which resulted in lower mortality of animals. The toxic effects of high doses, both PalGly and Pal-5-ALA, are characterized by a gender dependence: males were more sensitive to PalGly, whereas females - to Pal-5-Ala. Chronic administration of liposomes of phosphatidylcholine (without N-acylamino acids) caused the death of several mice, suggesting that the toxic effects of PalGly and Pal-5-ALA in liposomal form are at least partly due to the influence of the liposomes themselves.
The study of wound-healing effect of the gel substances with 2 and 4 % (w/w) concentrations of the dry extracts of the everlasting flowers and of the littlewale leaves in the ratio 1:1 were carried out.It was found that the skin application of the studied gel substances promoted the formation of the structural and functional regenerate in the area of the wound defect. The studied gel contributed to the significant reduction of the healing duration of the full-thickness linear wounds in rats compared to the control group of animals (without treatment) and to the group of animals that received treatment with the reference listed drug. The use of the studied gels, regardless of the content of the plant extracts in them (2 or 4 % (w/w)), was not accompanied by toxic effects.
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