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The allelic diversity in seed storage proteins of 25 bread wheat cultivars grown in Hellas was investigated. In total, 15-20 seeds per cultivar were used for the determination of the alleles present at the loci coding for high-molecular-weight glutenin subunits and gliadins. For this purpose, acid polyacrylamide gel electrophoresis for gliadins and SDSelectrophoresis for glutenins were employed. Analysis of the electrophoretic patterns revealed that intravarietal selections obtained from the cultivar ÔNestosÕ, together with the cultivar ÔEurydiceÕ which was selected from the cultivar ÔNestosÕ, were identical to their original cultivar and to the cultivar ÔDodoniÕ. The cultivars ÔPeniosÕ, ÔSiette Cerros EÕ, ÔGorgonaÕ and ÔLourosÕ, although recorded to differ in descent, were found to be identical at all the loci examined. Finally, it was revealed that four of the Hellenic cultivars carry the wheat-rye 1BL/1RS translocation. These data could be beneficial for a better understanding of the existing differences in quality and stress-resistance between the cultivars examined.

Genotypes at the gliadin loci Gli

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Intron-specific DNA polymorphism is present among plant β-tubulin gene family members and is considered to be one of the molecular markers based on the difference of tubulin introns length assayed both separately (TBP: 1st intron) or in combination (h-TBP: 1st and 2nd introns). These two approaches are possibly useful for wheat breeding programs, since TBP and h-TBP help to differentiate between the accessions of Aegilops biuncialis Vis., a wild relative of wheat. PCR-derived polymorphic fragments were resolved by PAGE electrophoresis. The length of amplicons varied significantly (395-3900 bp for TBP and 466-3440 bp for h-TBP), while the numbers of polymorphic bands were 21 for TBP and 23 for h-TBP, respectively. PIC mean value was circa 0.3. Dendrograms constructed on the basis of the Nei and Li coefficient with the high bootstrap support reveal a similar order of hierarchy for the samples analyzed using both methods. Thus, both techniques uncover DNA polymorphism level sufficiently high to distinguish different accessions of Ae. biuncialis Vis.

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