Variation at storage protein loci in winter common wheat cultivars of the central forest-steppe of Ukraine

Козуб, Н. А.; Созинов, И. А.; Sobko, T. A.; Kolyuchii, V. T.; Kuptsov, S. V.; Sozinov, A. A.

doi:10.3103/s0095452709010101

Cited by 35 publications

(22 citation statements)

References 17 publications

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“…However, it is interesting that the Gli-D1 b allele originates from a very different genepool of winter wheat, rather than spring wheat. This statement is based on published data showing a quite high distribution of the allele b among winter wheat, but not in spring wheat, in the former Soviet Union (Kozub et al, 2009; Novoselskaya-Dragovich et al, 2015). Therefore, we can speculate that the possible introgression of the Gli-D1 b allele from winter wheat can indicate for the wide adaptability of wheat genotypes, regardless of their responses to cold and vernalisation.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of gliadin-coding alleles in bread wheat (Triticum aestivum L.) from Northern Kazakhstan

Utebayev

Dashkevich

Bome

et al. 2019

PeerJ

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Background Spring bread wheat (Triticum aestivum L.) represents the main cereal crop in Northern Kazakhstan. The quality of wheat grain and flour strongly depends on the structure of gluten, comprised of gliadin and glutenin proteins. Electrophoresis spectra of gliadins are not altered by environmental conditions or plant growth, are easily reproducible and very useful for wheat germplasm identification in addition to DNA markers. Genetic polymorphism of two Gli loci encoding gliadins can be used for selection of preferable genotypes of wheat with high grain quality. Methods Polyacrylamide gel electrophoresis was used to analyse genetic diversity of gliadins in a germplasm collection of spring bread wheat from Northern Kazakhstan. Results The highest frequencies of gliadin alleles were found as follows, in Gli1: -A1f (39.3%), -B1e (71.9%), and -D1a (41.0%); and in Gli-2: -A2q (17.8%), -B2t (13.5%), and -D2q (20.4%). The combination of these alleles in a single genotype may be associated with higher quality of grain as well as better adaptation to the dry environment of Northern Kazakhstan; preferable for wheat breeding in locations with similar conditions.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity of gliadin-coding alleles in bread wheat (Triticum aestivum L.) from Northern Kazakhstan

Utebayev

Dashkevich

Bome

et al. 2019

PeerJ

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“…Gliadin electrophoresis was performed in an acidic medium in a polyacrylamide gel (Kozub, 2001). The gliadin allele Gli-B1l (Gli-B1-3) was a genetic marker of wheat-rye 1BL/1RS translocation (Kozub, 2009). The Gli-B1l allele was identified by the catalog (Metakovsky, 1991).…”

Section: Methodsmentioning

confidence: 99%

Identification of prospective sources of agronomically-valuable traits of bread wheat (Triticum aestivum L.) among breeding lines in the condition of Forest-Steppe of Ukraine

Kovalchuk¹,

Voloschuk²,

Козуб³

et al. 2020

Ukr J Ecol

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The aim of work was the estimation of valuable traits of bread wheat breeding lines, obtained from interspecies crosses with wild Aegilops and Triticum species growing in a condition of the Forest-Steppe of Ukraine. We used the seed proteins electrophoresis in PAAG for confirmation of the presence of rye seed storage components in the wheat parental lines genomes. The biochemical compositions of seeds had determined by the infrared spectroscopy method. As a result of researching from the set of 600 breeding lines were selected best lines with increased grain yield from 1 m2, with high protein content in grain, disease resistance, and winter hardiness significantly exceeded the standard variety Polesskaya-90. All lines have high and moderate resistance against diseases: Powdery Mildew, Brown Rust, Septoria Blotch. Based on obtained data had selected breeding lines, which were promising sources of single and complex agronomically valuable traits for bread wheat breeding and genetic researches.

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“…Встановлено високий рівень мінливості за гліадинкодуючими локусами хромосом першої гомеологічної групи. Найбільш поліморфними виявилися локуси Gli-A1 та Gli-B1 за якими було виявлено 11 Переважаючими ці алельні варіанти були і в групах сортів місцевих пшениць Степу і Лісостепу. Водночас спостерігаються певні відмінності між групами сортів за частотами інших алелей.…”

Section: матеріали і методиunclassified

“…Методологія використання генетично зумовленого поліморфізму локусів запасних білків для дослідження злакових культур та принципи генетичної класифікації гліадинів, які було розроблено українськими вченими майже пів сторіччя тому, сприяли появі нового напрямку у генетико-селекційних дослідженнях культури [4,5]. На сьогодні локуси запасних білків широко використовуються для дослідження сортового генофонду м"якої пшениці різного географічного походження, для ідентифікації генотипу та аналізу сортової чистоти, як маркери господарсько-цінних ознак у селекції культури [5][6][7][8][9][10][11]. Ефективними генетичними маркерами в цих дослідженнях є гліадинкодуючі локуси, зокрема локуси хромосом 1 гомеологічної групи.…”

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Genetic diversity of ukrainian wheat landraces Triticum aestivum L. at gliadin-coding loci

Sobko¹,

Lisova²,

Sirant³

2020

Fakt. eksp. evol. org.

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Aim. The aim of the study was to investigate genetic variability of gliadin-coding loci Gli-A1, Gli-B1, Gli-D1 in Ukrainian winter common wheat landraces and obsolete varieties. Methods. APAG electrophoresis of gliadin was used to identify genotypes at Gli-1 loci. Results. Diversity at the Gli-1 loci of 41 winter common wheat landraces, including local varieties Krymka, Banatka, Girka, Theyka, and 13 obsolete winter common wheat varieties, were studied. A total 31 gliadin alleles (including new 7 alleles) were revealed. There are 11 alleles at the Gli-A1 loci, 14 - at the Gli-B1, 6 -at the Gli-D1. Differences in frequencies of gliadin alleles were established. Conclusions. The high level of allelic variation at the Gli-1 loci was observed in Ukrainian winter common wheat landraces. Predominant alleles were revealed: Gli-A1m (46,8 %), Gli-A1d (21 %), Gli-B1b (47,1 %), Gli-D1g (45,8 %), Gli-D1a (33,3 %), Gli-D1j (13,9 %). In genotype of modern Ukrainian winter common wheat are present only 3 of them - Gli-B1b, Gli-D1g, Gli-D1j, which positively relate to dough quality. Keywords: Triticum aestivum L., winter wheat, landraces, gliadin , alleles , polymorphism

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Variation at storage protein loci in winter common wheat cultivars of the central forest-steppe of Ukraine

Abstract: Genotypes at the gliadin loci Gli

Cited by 35 publications

References 17 publications

Genetic diversity of gliadin-coding alleles in bread wheat (Triticum aestivum L.) from Northern Kazakhstan

Genetic diversity of gliadin-coding alleles in bread wheat (Triticum aestivum L.) from Northern Kazakhstan

Identification of prospective sources of agronomically-valuable traits of bread wheat (Triticum aestivum L.) among breeding lines in the condition of Forest-Steppe of Ukraine

Genetic diversity of ukrainian wheat landraces Triticum aestivum L. at gliadin-coding loci

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