The primary structure of a gene of the Na +,K+-ATPase multigenic family in the human genome has been determined. The gene corresponds to a hypothetical ~dlI-form of the enzyme catalytic subunit. The gene comprises over 25000 bp, and its protein coding region includes 23 exons and 22 introns. Possible correlation between structural features of the protein and location ofintrons in the gene are discussed.

Five different nucleotide sequences have been found in the human genome homologous to the gene of the α‐subunit of Na+,K+‐ATPase. A comparative analysis of the primary structure of these genes in the region 749–1328 (in coordinates of cDNA from the pig α‐subunit) is presented.

cDNA libraries enriched with sequences that are differentially transcribed in normal and tumor tissues were prepared using the subtractive hybridization of mixtures of cDNAs from ten patients with squamous cell lung cancer and the corresponding mixtures of cDNAs from normal tissues of the same patients. An analysis of the libraries revealed two genes, NOLA2 and RPS3A, whose expression in patients with squamous cell lung cancer increased by 70%. A high frequency of enhanced expression of these genes in the cancer makes them highly informative markers of squamous cell lung cancer, which, together with other markers, can be used for reliable diagnosis of the disease.

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