The family of human Na⁺,K⁺‐ATPase genes No less than five genes and/or pseudogenes related to the α‐subunit

Abstract

Five different nucleotide sequences have been found in the human genome homologous to the gene of the α‐subunit of Na+,K+‐ATPase. A comparative analysis of the primary structure of these genes in the region 749–1328 (in coordinates of cDNA from the pig α‐subunit) is presented.

Advances in Na+,K+‐ATPase studies: from protein to gene and back to protein

“…Only one sequence was found in the kidney of sheep [15] and pigs [16,19]. However, further investigations of chromosomal genes for the a-subunit of human Na+,K+-ATPase [28,29], as well as of the screening of cDNA library from rat brain [30] have led to the discovery of the gene family. At present, probably, about seven sequences, highly homologous to the gene for the a-subunit, were cloned in the human genome [29,31].…”

“…However, further investigations of chromosomal genes for the a-subunit of human Na+,K+-ATPase [28,29], as well as of the screening of cDNA library from rat brain [30] have led to the discovery of the gene family. At present, probably, about seven sequences, highly homologous to the gene for the a-subunit, were cloned in the human genome [29,31]. Out of these genes, three correspond to the three mRNAs (aI, ~~11, aIII), found in rat brain which code for three isoforms of the enzyme a-subunit [30].…”

“…Out of these genes, three correspond to the three mRNAs (aI, ~~11, aIII), found in rat brain which code for three isoforms of the enzyme a-subunit [30]. One of the genes appeared to be the gene encoding H+,K+-ATPase [29,32]. The other genes are not identified [29,31].…”

Family of human Na+,K+‐ATPase genes Structure of the putative regulatory region of the α+‐gene

“…At least five genes and/or pseudogenes, highly homologous to the a-subunit of Na+,K+-ATPase [3], have been shown to exist in the human genome, the expression of which is tissue-specific [4]. The tissue specificity of their expression can be regulated at different levels including the regulation of transcription [5].…”

Immunodetection of Na,K‐ATPase α3‐isoform in renal and nerve tissues

“…The enzyme molecule consists of two polypeptides, catalytic a-subunit and glycoprotein ~' of unknown function (for review see [1]). To date, the number of known genes for the Na,K-ATPase catalytic subunit [2] exceeds that of identified protein isoforms (only cr and a~+ subunits have been characterized by biochemical methods [3]). A complete nucleotide sequence of the gene for the ot3-isoform of the catalytic subunit is established [4], the gene expression in various organs and tissues is studied (a,3 mRNA is detected in rat brain [5], stomach, lung, liver and fetal heart [6], human kidney [7]).…”