Family of human Na<sup>+</sup>,K<sup>+</sup>‐ATPase genes Structure of the gene for the catalytic subunit (αIII‐form) and its relationship with structural features of the protein

Ovchinnikov, Yu.A.; Monastyrskaya, G.S.; Broude, Natalia E.; Ushkaryov, Yuri A.; Melkov, A.M.; Smirnov, Yu.V.; Malyshev, I.V.; Allikmets, Rando; Костина, М. Б.; Dulubova, Irina; Kiyatkin, Nikita; Grishin, A. V.; Modyanov, Nikolaï N.; Свердлов, Е. Д.

doi:10.1016/0014-5793(88)81361-9

Cited by 69 publications

(32 citation statements)

References 34 publications

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“…The structure of 3 '-and 5 '-splice sites coincides with the consensus sequence described in [43], many introns of the ~~111 gene correspond to hydrophilic amino acids (table 1, which, in a different way, presents the data published in [40]). …”

Section: The Structure Of the Gene Of ~~111 Isoform In Connection Witmentioning

confidence: 53%

“…One of the genes belonging to the family of a-subunit genes was isolated in four overlapping clones [40,41]. The analysis of the primary structure of the exons of this gene revealed that it codes for a protein which is 99% homologous with the a111 isoform of the rat brain catalytic subunit [30].…”

Section: The Structure Of the Gene Of ~~111 Isoform In Connection Witmentioning

confidence: 99%

“…The analysis of the primary structure of the exons of this gene revealed that it codes for a protein which is 99% homologous with the a111 isoform of the rat brain catalytic subunit [30]. The reconstructed fragment of overall size of 30 kb contains the total coding sequence [40,41]. It consists of 23 exons separated by 22 introns.…”

Section: The Structure Of the Gene Of ~~111 Isoform In Connection Witmentioning

confidence: 99%

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Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein

Broude¹,

Modyanov²,

Monastyrskaya³

1989

FEBS Letters

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Complete primary stators of both submit of Na',K+-ATPase from various sources have been ~~biisbed by a ~mbination of the methods for molecular cloning and protein chemistry. The gene family homologo~ to the a-subunit cDNA of animal Na+,K*-ATPases has been found in the human genome. Some genes of this family encode the known isoforms (aI and aI1) of the Na+,K+-ATPase catalytic subunit. The proteins coded by other genes can be either new isoforms of the Na+,K+-ATPase catalytic subunit or other ion-transporting ATPases. Expression of the genes of this family proceeds in a tissue-specific manner and changes during the postnatal development and neoplastic transformation. The complete exon-intron structure of one of the genes of this family has been established. This gene codes for the form of the catalytic subunit, the existence of which has been unknown. Apparently, all the genes of the discovered family have a similar intron-exon structure. There is certain correlation between the gene structure and the proposed domain a~angement of the a-subunit. The results obtained have become the basis for the experiments which prove the existence of the earlier unknown afI1 isoform of the Na+,K+-ATPase catalytic subunit and have made possible the study of its function.

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Section: The Structure Of the Gene Of ~~111 Isoform In Connection Witmentioning

confidence: 53%

Section: The Structure Of the Gene Of ~~111 Isoform In Connection Witmentioning

confidence: 99%

Section: The Structure Of the Gene Of ~~111 Isoform In Connection Witmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein

Broude¹,

Modyanov²,

Monastyrskaya³

1989

FEBS Letters

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“…The comparison of the primary structures derived from the nucleotide sequences of o~ [11], + [12] and ~3 [4] revealed the notable difference in two regions ( fig. 1).…”

Section: Resultsmentioning

confidence: 99%

“…To date, the number of known genes for the Na,K-ATPase catalytic subunit [2] exceeds that of identified protein isoforms (only cr and a~+ subunits have been characterized by biochemical methods [3]). A complete nucleotide sequence of the gene for the ot3-isoform of the catalytic subunit is established [4], the gene expression in various organs and tissues is studied (a,3 mRNA is detected in rat brain [5], stomach, lung, liver and fetal heart [6], human kidney [7]). All these underlay the search for a pro- tein product of the a3 gene expression.…”

Section: Introductionmentioning

confidence: 99%

Immunodetection of Na,K‐ATPase α3‐isoform in renal and nerve tissues

1989

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At least three types of mRNA of the catalytic subunit of Na,K-ATPase namely ~t-,~t+-and ~t3-isoforms are identified in different tissues. Only two of them ~t and ~t + have well known structural and catalytic properties. Here we present immunochemical data indicating that the ~t3 protein really exists in pig and human kidney, and human brain. Crude membrane fractions and purified membrane-bound Na,K-ATPases were immunoblotted with ~t3-specific antibodies raised against the synthetic peptide corresponding to the unique sequence of this isoform. The mature ~t3-subunit is shown to include the sequence GDKKDDKSSPK followed by the initiating methionine residue. Nephron collecting tubules are proposed to specifically contain Na,K-ATPase ~t3-isoform.

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Ouabain sensitivity and expression of Na/K-ATPase α- and β-subunit isoform genes during bovine early development

et al. 1997

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The fluid movements that arise during blastocyst formation (cavitation) are, at least in part, driven by the Na/K‐ATPase. In this study, the reverse transcriptase‐polymerase chain reaction (RT‐PCR) was used to survey bovine pre‐attachment embryos for transcripts encoding known isoforms of the Na/K‐ATPase α‐ and β‐ subunits, including isoforms not previously detected during the first week of mammalian development. Transcripts encoding the Na‐K‐ATPase α1, α2, α3 and β2 isoforms were detected throughout bovine preattachment development. This is the first indication that α2, α3 and β2 mRNAs are expressed during this early developmental interval. As in the mouse, β1‐subunit transcripts were not detected until the morula stage and were also present in blastocysts. Thus, in two mammalian species an increase in abundance of β1 isoform transcripts in the morula stage is coincident with the onset of cavitation. Transcripts encoding the recently characterized α4 isoform were not detected. The sensitivity of bovine blastocysts to ouabain (a potent inhibitor of Na/K‐ATPase) was determined by assessing the ability of bovine blastocysts to recover in ouabain supplemental culture medium following cytochalasin‐induced blastocyst collapse. Re‐expansion of bovine blastocysts was inhibited in all ouabain concentrations down to 10 9 M. Mouse blastocysts, in contrast, were sensitive to ouabain at or above 10 3M. These results have established that transcripts encoding multiple isoforms of both the α and β subunits of the Na/K‐ATPase are expressed throughout early bovine development and that bovine blastocysts display a greater sensitivity to ouabain than murine blastocysts. Future analysis will determine the possible individual and collective roles of these isoforms during blastocyst formation. Mol Reprod Dev 46:114–126, 1997. © 1997 Wiley‐Liss, Inc.

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Family of human Na⁺,K⁺‐ATPase genes Structure of the gene for the catalytic subunit (αIII‐form) and its relationship with structural features of the protein

Cited by 69 publications

References 34 publications

Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein

Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein

Immunodetection of Na,K‐ATPase α3‐isoform in renal and nerve tissues

Ouabain sensitivity and expression of Na/K-ATPase α- and β-subunit isoform genes during bovine early development

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Family of human Na+,K+‐ATPase genes Structure of the gene for the catalytic subunit (αIII‐form) and its relationship with structural features of the protein

Cited by 69 publications

References 34 publications

Advances in Na+,K+‐ATPase studies: from protein to gene and back to protein

Advances in Na+,K+‐ATPase studies: from protein to gene and back to protein

Immunodetection of Na,K‐ATPase α3‐isoform in renal and nerve tissues

Ouabain sensitivity and expression of Na/K-ATPase α- and β-subunit isoform genes during bovine early development

Contact Info

Product

Resources

About

Family of human Na⁺,K⁺‐ATPase genes Structure of the gene for the catalytic subunit (αIII‐form) and its relationship with structural features of the protein

Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein

Advances in Na⁺,K⁺‐ATPase studies: from protein to gene and back to protein