Highlights d Comprehensive genetic and physical interactome of nuclear HSP90 in human cells d HSP90 stabilizes HCFC1 complex at chromatin to drive cellcycle gene expression d Synergistic killing of cancer cells by simultaneous inhibition of HSP90 and HCFC1

We studied the effect of inhibition of mitochondrial voltage-dependent anion channels with DIDS on radiosensitivity and mitochondrial status of K562 leukemic cells. The number of apoptotic and necrotic cells, mitochondrial transmembrane potential, and mitochondrial mass were evaluated after irradiation of cells in doses of 4 and 12 Gy in the presence and absence of the inhibitor. Inhibition of mitochondrial voltage-dependent anion channels increased radiosensitivity of K562 cells by 50-70% and decreased both mitochondrial transmembrane potential and mitochondrial mass. Inhibitors of voltage-dependent anion channels are promising agents capable of improving the effectiveness of cancer radiotherapy.

The dynamics of oxidative stress, DNA damage, changes in mitochondrial potential and mitochondrial mass are studied with the cancer cells of HELA line irradiated by a Raman laser at the wavelength of 1265 nm. It is demonstrated that Raman laser irradiation at this wavelength induces cell death causing an increase of the concentration of intracellular reactive oxygen species, an increase of DNA damage, and a decrease of mitochondrial potential and mitochondrial mass.