Gamma (γ)-irradiation of plants at low doses can provoke a broad range of growth-stimulating effects. In order to reveal universal target genes that are involved in molecular pathways of radiation hormesis establishment, we studied nine barley cultivars for their tolerance to γ-irradiation of seeds. Four morphological traits were assessed in barley seedlings after γ-irradiation of seeds at 20 Gy. Nine cultivars were sorted according to the sensitivity to irradiation as γ-stimulated, “no morphological effect”, or γ-inhibited. Gene expression of 17 candidate genes was evaluated for the 7 most contrasting cultivars. Changes in expression of barley homologues of PM19L and CML31 were suggested as possible determinants of radiation hormesis effect. The possible role of jasmonate signaling in roots in radiation growth stimulations was revealed. Morphological analysis and gene expression study showed that the genetic background of a cultivar plays an important role in eustress responses to low-dose γ-irradiation of seeds.
Low-dose γ-irradiation can stimulate plant growth and development; however, the knowledge on the molecular mechanisms of such stimulation is yet fragmented. Irradiation of seeds leads to the mobilisation of endosperm resources and reallocation of available nitrogen to facilitate development. Based on the metabolomic analysis, several metabolites possibly involved in radiation stimulation were studied using the HPLC approach in barley cultivars after γ-irradiation of seeds. The comparison of changes in metabolite concentrations and changes in morphological traits after irradiation revealed seven metabolites that may be involved in the growth stimulation after γ-irradiation of barley seeds. Among them are free amino acids, such as γ-aminobutyric acid, β-alanine, arginine, lysine, glutamine, methionine, and a signalling compound methylglyoxal.
Net form of net blotch (NFNT) is the most dangerous and devastating disease of barley causing huge losses in Russia. Highly effective gene rpt 5 determining resistance to net blotch pathogen in Russia was used in the study. A set of 95 winter barley indigenous and exotic varieties were studied to identify the genotypes with the alleles of 153 and 155 bp for the locus bmag173 and the alleles of 186, 188, 192 bp for the locus hvm74 exhibiting resistance to the net blotch pathogen (Pyrenophora teres Drechs. f. teres) occurring in North Caucasus region of Russia.
The article presents the main biologic and economic advantages of winter barley, shows the main directions of its use in the industrial processing. The article informs about the dynamics of the sown area and productivity of winter barley in Russia, their distribution through Federal Districts. There has been established that the North-Caucasus and Southern Federal Districts are the main winter barley producers with 153.6–245.6 thousand hectares and 131.2–28.7 thousand hectares respectively. The use of great number of seeds of mass reproduction together with low sowing traits significantly reduces productivity and qualitative characteristics of the obtained products. The article gives the analysis of winter barley seed-growing from 2012 to 2017. There has been analyzed data on the amount of original sown (OS-1.2), basic (BS) and reproductive seeds (RS1, RS2, RS3). There has been determined a tendency to an increase in the volume of sown seeds of winter barley in the total area from 54.3 thousand hwt in 2013 up to 74.1 thousand hwt in 2016. The largest amount of sown seeds of the basic seeds (14.1 thousand hwt) took place in 2015, the smallest one (6.3 thousand hwt) occurred in 2017. It was found that the number of reproductive seeds remains high and ranges from 78.8 to 88.5% with an annual reduction in volumes in absolute terms. In this regard, there is a periodical need to update the seeds of the used winter barley varieties. In order to stabilize the winter barley productivity, it is necessary to sow the seeds of varieties recommended for sowing in the Rostov region by the State Commission for Variety Testing.
Barley is the main forage crop in the Russian Federation. Barley grain is also used for food and brewery (Filippov, 2013). It is common knowledge that low doses of gamma irradiation can have a stimulating effect on the growth and development of plants, in particular barley. However, there is a lack of knowledge about the molecular pathways responsible for the formation of the stimulation effect after low-dose seed irradiation. The use of varieties with different radio sensitivity to low-dose gamma irradiation will allow studying specific molecular mechanisms to form a stimulating irradiation effect. Such knowledge could further help in the development of varieties with a large stable productivity over the years and with high resistance to biotic and abiotic stressors. In 2019 there was conducted a gamma irradiation of the original seeds of nine winter and spring barley varieties developed in the FSBSI “Agricultural Research Center “Donskoy” in order to assess intensity of the response on low-dose ionizing irradiation based on changes in the morphological parameters of irradiated and unirradiated plants. The original seeds of each barley variety were irradiated at the FSBSI “All-Russian Research Institute of Radiology and Agroecology” by the gamma device “GUR-120” with 60Со radiation sources at a dose of 20 Gy (dose rate of 60 Gy/hour). The statistical data processing was performed by the Microsoft Office Excel 2019. The morphological analysis of the length and weight of roots and sprouts made it possible to establish the presence or absence of the stimulating irradiation effect on the studied indicators of the varieties and to conduct their subsequent ranking according to the value of the radiobiological effect. There have been identified the winter and spring barley varieties with different sensitivity to a dose of 20 Gy. The stimulating irradiation effect was established in such varieties as “Foks 1”, “Ratnik”, “Yerema” and “Master”. The variety “Leon” had an inhibitory irradiation effect.
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