Studying Gene Expression in Irradiated Barley Cultivars: PM19L-like and CML31-like Expression as Possible Determinants of Radiation Hormesis Effect

Abstract

Gamma (γ)-irradiation of plants at low doses can provoke a broad range of growth-stimulating effects. In order to reveal universal target genes that are involved in molecular pathways of radiation hormesis establishment, we studied nine barley cultivars for their tolerance to γ-irradiation of seeds. Four morphological traits were assessed in barley seedlings after γ-irradiation of seeds at 20 Gy. Nine cultivars were sorted according to the sensitivity to irradiation as γ-stimulated, “no morphological effect”, or γ-inhibited. Gene expression of 17 candidate genes was evaluated for the 7 most contrasting cultivars. Changes in expression of barley homologues of PM19L and CML31 were suggested as possible determinants of radiation hormesis effect. The possible role of jasmonate signaling in roots in radiation growth stimulations was revealed. Morphological analysis and gene expression study showed that the genetic background of a cultivar plays an important role in eustress responses to low-dose γ-irradiation of seeds.

Zeocin-induced DNA damage response in barley and its dependence on ATR

“…SOG1 is a member of a large NAC (NAM, ATAF1/2, and CUC2) family of transcription factors in plants and a list of 167 barley NAC domain containing proteins was published, highlighting HORVU.MOREX.r3.7HG0670800 (alias HORVU7Hr1G042420) as the most likely homolog of Arabidopsis ANAC008 ( At SOG1) 24 . On contrary, another gene HORVU.MOREX.r3.6HG0590960 (alias HORVU6Hr1G053540) was suggested as HvSOG1 based on upregulation upon DNA damage treatment 25 . Therefore, we analyzed SOG1 in barley in more detail.…”

Machine Learning for Plant Stress Modeling: A Perspective towards Hormesis Management

“…Therefore, transcriptomics is the key to investigating gene function in targeted physiological mechanisms qualitatively and quantitatively [ 57 ]. Detecting hormetic stimulation at the transcript level can be achieved by analyzing the differential expression of known genes on small (~20) [ 58 , 59 , 60 ] and very large scales using microarray technology (~50,000) [ 61 ] or by completely sequencing the RNA from a sample as in next-generation and third-generation sequencing [ 62 , 63 , 64 ].…”

Comparative Analysis of the Effect of Gamma-, Electron, and Proton Irradiation on Transcriptomic Profile of Hordeum vulgare L. Seedlings: In Search for Molecular Contributors to Abiotic Stress Resilience

“…Radiobiological studies of barley responses to IR showed that this stress factor can be successfully used to identify molecular pathways involved in barley adaptation to abiotic stressors [23][24][25]. Numerous papers are focused on barley's adaptive responses to γ-radiation, as it is the radiation type more available for research [9,[23][24][25][26][27][28], where growthstimulating and growth-inhibiting dose-dependent effects are considered in molecular details.…”

“…Radiobiological studies of barley responses to IR showed that this stress factor can be successfully used to identify molecular pathways involved in barley adaptation to abiotic stressors [23][24][25]. Numerous papers are focused on barley's adaptive responses to γ-radiation, as it is the radiation type more available for research [9,[23][24][25][26][27][28], where growthstimulating and growth-inhibiting dose-dependent effects are considered in molecular details. Several studies of proton and electron radiation effects on barley plants are also available in the literature, demonstrating both positive and negative impacts on its growth, development, and productivity, alone or in combination with other stress factors [29,30].…”