Blood sera of 12 severe Covid-19 patients and 14 healthy human donors were subjected to original TCA-extraction/acetone-precipitation followed by SDS-PAAG electrophoresis and mass-spectrometry. 76 kDa protein was detected as one of the differentially expressed proteins in the samples of Covid-19 patients. This 76 kDa protein was identified with mass-spectrometry as human serum albumin. Such molecular form of albumin was absent in blood serum of healthy human donors. The potential ways of generation of the unusual form of human serum albumin and its probable diagnostic value were discussed. Keywords: acetone-precipitation, biomarker proteins, COVID-19, electrophoresis, mass-spectrometry, TCA-extraction, unusual form of human serum albumin
The body of PLHIV undergoes profound anthropometric changes in adipose tissue distribution, which develop under the influence of pathogenetic mechanisms caused by HIV and due to impaired side effects of ART. ART leads to the development of lipoatrophy / lipohypertrophy syndrome. This phenomenon is described as a syndrome characterized by the loss and/or accumulation of fat and has three types: lipohypertrophy, lipoatrophy, and mixed lipodystrophy. These changes in body shape are very important to determine because they are associated with negative disease dynamics and high mortality. Therefore, it is extremely important to detect this syndrome early to ensure a better quality of life for this population, as the clinical approach is not easy. Potential approaches to treatment, including lifestyle changes, with adequate eating habits, exercise, and some medical interventions, showed little effect in PLHIV. In this case can be effectively reduced through exercise. Despite the existence of established exercise guidelines, the effective dosage of exercise to reduce HRT requires verification. The aim. Study the anthropometric parameters in PLHIV and establish the relationship between the parameters and the degree of viral load (HV) of HIV, the level of CD4 + T-lymphocytes. Materials and methods: 60 patients aged 18 to 60 years were examined. We used standard methods of measuring the following anthropometric indicators: body weight, height, chest circumference, waist and hips. The waist-to-thigh ratio index was also determined. The calculation of the body mass index was performed according to the standard formula by dividing body weight (in kilograms) by the square of height (in square meters). The indicator 18.5-24.9 is considered normal. The level of CD4 + T-lymphocytes was determined by flow cytofluorometry. The viral load of HIV was determined by real-time PCR. Statistical indicators, median, Pearson's coefficient, Student's t-test, were calculated using Microsoft Excel 2016. Results. The average waist circumference was smaller in both the men of the experimental group (74.5 ± 10.3 cm) and the women of the experimental group (61.9 ± 11.0 cm) than in the control groups of men (90.4 ± 9.4 cm) and women (67.5 ± 7.3 cm (p <0.05)). The average ratio of waist circumference to hip circumference in the group of male PLHIV was 0.94 ± 0.08 cm in the control group of men - 0.97 ± 0.8 cm (p <0.05). In the group of female PLHIV, this indicator reached 0.86 ± 0.1 cm, and in the control group - 0.75 ± 0.3 cm (p <0.05). The mean forearm circumference was lower in the two research groups. However, in men with HIV (18.5 ± 3.1 cm) this figure was lower than in women with HIV (19.9 ± 3.9 cm (p <0.05)). Also, the circumference of the tibia was larger in female PLHIV (26.9 ± 3.9 cm) than in male PLHIV (20.8 ± 3.8 cm (p <0.05)). Conclusions. PLHIV at the stage of AIDS leads to the recomposition of the body. It is manifested by a decrease in BMI, waist circumference, hip, mid-upper arm, and calf, which indicates lipodystrophy. Increase in the index of the waist circumference to hip circumference ratio indicates a probable redistribution of adipose tissue. BMI cannot be used as an accurate method to determine obesity or lipodystrophy. A positive correlation was found between the value of the anthropometric profile and the level of viral load and CD4 + T-lymphocytes. Early administration of ART and dosed exercise is likely to have a positive effect on the body structure of PLHIV in the AIDS stage, although this requires further research.
Introduction. People living with HIV (PLWH) have a great need in physical therapy (PT), but there are many barriers to accessing it. Specific recommendations on how to integrate PT into multidisciplinary teams and outpatient care programs PLWH is currently in short supply. The aim of the study. Identify factors to consider when integrating PT into the work of a multidisciplinary team in providing assistance to PLWH. Materials and methods. A descriptive sociological study consisting of interviews with health workers (n=12) and with adults living with HIV (n=13). Research results and their discussion. Guided by the principles of biopsychosocial models in the study of barriers and factors to be considered when integrating PT in multidisciplinary team for the management of PLWH, identified 8 contextual factors that may affect the integration of PT in the care of PLWH. Conclusion. The role of PT in the care of PLWH is multifactorial and patient-oriented, a special role belongs to the contextual factors. Prospects for further research. Structuring the PT mode with orientation on a specific patient; development of methods for assessing PT as an interprofessional component assistance.
Coronavirus disease 19 (COVID-19) is a global health crisis on a planetary scale. COVID-19 in many people has mild or moderate manifestation, although significant number of people, especially the elderly, suffer heavy from this illness, which often resulting in death. There are reports of similarities in immune response between COVID-19 and some autoimmune diseases. Earlier, we have demonstrated that fraction of TCA-soluble blood serum proteins containing a 48 kDA fragment of unconvential Myosin C1 have linked with development of multiple sclerosis and rheumatoid arthritis. Here we analyze use of these proteins in determining the severity of disease in COVID-19 patients. We found that blood serum of COVID-19 patients in acute disease manifestation contains, in contrast to healthy individuals, the TCA-soluble proteins with molecular masses 48 kDa and 76 kDA which were identified as a short form of unconventional myosin 1c and a modified form of human serum albumin.
Purpose: to assess the functional state of patients with human immunodeficiency virus with different levels of T-lymphocytes for use in the practice of physical therapy. Materials and methods. We examined 24 patients with human immunodeficiency virus at the stage of acquired immunodeficiency syndrome at the outpatient stage of treatment. The patients were divided into 2 groups. Group 1 included 11 patients with CD4+ T-lymphocyte levels <100 cells ml-1, and group 2 included 13 patients with CD4+ T-lymphocyte levels above 150 cells ml-1 (р<0.01). The clinical and anamnestic data, the main anthropometric parameters and physiological indices, the results of carpal dynamometry, the 6-minute walk test, characterizing the functional state of people living with the human immunodeficiency virus, were studied. The results of immunological, virological and biochemical parameters were also studied. Results. Anthropometric indicators: Group 1 – body mass index 48.9±7.4 kg m2-1, waist to hip ratio ratio 0.92±0.17 cm, leg circumference 16.6±0.8 cm; Group 2 – body mass index 23.1±2.8 kg m2 -1, waist to hip ratio ratio 1.14±0.11 cm, leg circumference 15.9±1.4 cm (р>0.05 ). Indicators of physiological indices: Reed - group 1 - 17.3±7.2%, group 2 - 18.3±11.8% (p>0.05); Hobbes - group I 70.9 ± 5.5%, group 2 - 93.6 ± 11.8% (p<0.05), Kerdo - group 1 was 26.6 ± 14.2%, group 2 - 10 .8 ± 14.7% (р<0.05). Index of functional changes group 2 - 2.44±0.2 points, group 2 - 2.59±0.3 points (p>0.05). Dynamometry indicators - group I - 17.5 ± 6.8 kg, group 2 - 28.1 ± 9.4 kg (р>0.05); strength index – group 1 35.3±-9.6%, group 2 40.5±10.4% (р>0.05); 6 min walk test in group 1 - 402.8±40.04 m, group 2 459.7±56.1 m (р<0.05). Indicators of biochemical studies were in reference values ??in patients of both groups. Conclusions. There is a decrease in the functional state and the predominance of catabolic processes in people with human immunodeficiency virus with a significant deficiency of CD4+ T-lymphocytes.
Резюме. Цілі: дослідити зв'язок втоми у людей що живуть з ВІЛ (ЛЖВ), побутової фізичної активності та факторами, які можуть на неї впливати. Матеріали і методи: Обстежено 60 ЛЖВ, 40 чоловіків і 20 жінок у віці від 23 до 61 року. Втому оцінювали за допомогою шкали FAS. Фізичну активність вивчали за допомогою щоденника фізичної активності, якість життя (ЯЖ) – опитувальником SF 36. Показник рівня CD4+ T-лімфоцитів визначали методом проточної цитофлуориметрії, вірусне навантаження (ВН) - за допомогою ПЛР. Результати дослідження та їх аналіз. Ступінь втоми у ЛЖВ коливалася від 17 до 47 балів, середнє значення – 36,4 ± 6,4 балів. До першої групи увійшло 46 пацієнтів з вираженою втомою, а до другої – 14 пацієнтів з помірною. У I групі встановлено: пряму кореляцію з вірусним навантаженням (r = 0,16, р = 0,02), позитивну кореляцію між психічним компонентом ЯЖ та тривалістю сну (r = 0,24, р = 0,06); зворотній зв'язок з фізичною побутовою активністю (r = -0,43, р = 0,0001), загальним показником ЯЖ (r = -0,16, p = 0,02), фізичним та психічним компонентами ЯЖ – r = -0,19, р = 0,03 та r = -0,37, р = 0,13 відповідно, рівнем CD4+ T-лімфоцитами та ВН (r = -0,25, р = 0,06). У II групі встановлено: пряму кореляцію між втомою та тривалістю сну r = 0.46, р = 0.22, ВН r = 0.24, р = 0.28; зворотна кореляція була між ступенем втоми та загальним показником ЯЖ r = -0.18, р = 0.03, фізичним компонентом ЯЖ r = -0.15, р = 0.02., рівнем CD4+ Т-лімфоцитів r = -0.27, р = 0.17, фізичною побутовою активністю r = -0.35, р = 0.005, ВН r = 0.53, р = 0.28. Висновки. Побутова фізична активність може зменшити втому у ЛЖВ. Тривалість сну позитивно впливає на досліджувані нами патерни здоров’я у ЛЖВ. Встановлено, що втома пов’язана з високим ВН, низькою ЯЖ та ІМТ. Виникає потреба у розробці математичної моделі оцінки втоми.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.