RNA interference (RNAi) is one of the most widely used techniques to study gene functions. There is still a lack of RNAi techniques that can be applied in Phytoseiidae conveniently and efficiently. Star Polycation is a new nanomaterial commonly used as a carrier of dsRNA in RNAi. Five genes of P. persimilis (PpATPb, PpATPd, PpRpL11, PpRpS2, and Pptra-2) were selected to verify whether SPc promotes the delivery of dsRNA into P. persimilis through soaking. When each of the five genes were interfered using SPc-mediated dsRNA, the total number of success offspring produced per female in six days decreased by ca. 92%, 92%, 91%, 96%, and 64%. When PpATPb, PpATPd, PpRpL11, or PpRpS2 was interfered, both the fecundity and egg hatching rate decreased. In contrast, when Pptra-2 was interfered, reduction in the reproductive capability was mainly the result of the decreased egg hatching rate. Correspondingly, when the target gene was interfered, P. persimilis expression of PpRpL11 reduced by 63.95%, while that of the other four genes reduced by at least 80%. Our studies showed that nanomaterials, such as SPc, have the potential to be used in RNA interference of phytoseiid mites.
In the context of digitalization, it is particularly effective to export and disseminate cultural content in the way of game interaction. In particular, the emergence of HTML5 new communication carrier is favored by the digital display and dissemination of intangible cultural heritage in a more lightweight way and accepted easily by users. It can not only show difficult and old content, but also add interest and audio-visual interaction through small games. This chapter discusses this topic. Firstly, this chapter analyzes the interactive characteristics of the current functional intangible cultural heritage games applet based on HTML5,and summarizes its technical characteristics. Secondly, it makes a case analysis about the interactive framework of intangible cultural heritage games based on HTML5. Finally, it refines and summarizes the main art design process of HTML5. The chapter is committed to giving full play to the advantages of HTML5-based large user base, convenient communication, and open operation mode, so as to meet the users’ experience of intangible cultural heritage and game interactive experience, so as to promote the inheritance of intangible cultural heritage.
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