Flap
endonuclease 1 (FEN1), an endogenous nuclease with the ability
to cleave the 5′ overhang of branched dsDNA, is of significance
in DNA replication and repair. The overexpression of FEN1 is common
in cancer because of the ubiquitous upregulation of DNA replication;
thus, FEN1 has been recognized as a potential biomarker in oncological
investigations. However, few analytical methods targeting FEN1 with
high sensitivity and simplicity have been developed. This work developed
a signal-amplified detection of FEN1 based on the cleavage-induced
ligation of a dumbbell DNA probe and rolling circle amplification
(RCA). A flapped dumbbell DNA probe (FDP) was rationally designed
with a FEN1 cleavable flap at the 5′ end. The cleavage generated
a nick site with juxtaposed 5′ phosphate and 3′ hydroxyl
ends, which were linkable by T4 DNA ligase to form a closed dumbbell
DNA probe (CDP) with a circular conformation. The CDP functioned as
a template for RCA, which produced abundant DNA that could be probed
using SYBR Green I. The highly sensitive detection of FEN1 with a
limit of detection of 15 fM was achieved, and this method showed high
specificity, which enabled the quantification of FEN1 in real samples.
The inhibitory effects of chemicals on FEN1 were also evaluated. This
study represents the first attempt to develop an FEN1 assay that involves
signal amplification, and the novel biosensor method enriches the
tools for FEN1-based diagnostics.
Nitrobenzene compounds are highly toxic pollutants with good stability, and they have a major negative impact on both human health and the ecological environment. Herein, it was found for the first time that fluorescent DNA-silver nanoclusters (DNA-AgNCs) can catalyze the reduction of toxic and harmful nitro compounds into less toxic amino compounds with excellent tolerance to high temperature and organic solvents. In this study, the reduction of p-nitrophenol (4-NP) as a model was systematically investigated, followed by expending the substrate to disclose the versatility of this reaction. This report not only expanded the conditions for utilizing catalytic reduction conditions of DNA-AgNCs as an efficient catalyst in the control of hazardous chemicals but also widened the substrate range of DNA-AgNCs reduction, providing a new angle for the application of noble metal nanoclusters.
CRISPR-Cas12a is an accurate and responsive biosensing technique, but its limited stability has restricted its widespread applications. To address this, we propose a strategy using metal−organic frameworks (MOFs) to protect Cas12a from harsh environments. After screening multiple candidate MOFs, it was found that hydrophilic MAF-7 is highly compatible with Cas12a, and the as-formed Cas12a-on-MAF-7 (COM) not only retains high enzymatic activity but also possesses excellent tolerance to heat, salt, and organic solvents. Further investigation showed that COM can serve as an analytical component for nucleic acid detection, resulting in an ultrasensitive assay for SARS-CoV-2 RNA detection with a detection limit of 1 copy. This is the first successful attempt to create an active Cas12a nanobiocomposite that functions as a biosensor without the need for shell deconstruction or enzyme release.
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