Advancing sensing technology with CRISPR: From the detection of nucleic acids to a broad range of analytes – A review

Xie, Siying; Ji, Zhirun; Suo, Tiying; Li, Bingzhi; Zhang, Xing

doi:10.1016/j.aca.2021.338848

Cited by 48 publications

(21 citation statements)

References 128 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cas12 is divided into three subtypes: Cas12a (Cpf1), Cas12b (C2c1), and Cas12c (C2c3) (Shmakov et al., 2015). Of these, Cas12a is the most common (Xie et al., 2021). It is a single RNA‐required effector protein, and does not require tracrRNA.…”

Section: Classification Of Crispr/cas Systemsmentioning

confidence: 99%

“…CRISPR biosensing has been applied in diverse fields including biosecurity, food safety, life science, environmental monitoring, and medical diagnostics; and despite being in its early stages of development, it is widely expected to take food‐safety monitoring to the next level (Xie et al., 2021). For the food industry, in particular, the potential of this novel biosensing method is enormous, because in addition to bacterial pathogens it can be used to detect viruses, genetically modified organisms (GMOs), toxins, and food additives (Abnous et al., 2021; D. Huang et al., 2020; Jiao et al., 2021; Qiao et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in CRISPR‐based systems for the detection of foodborne pathogens

Shin

Miller

Wang

2022

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

There has long been a need for more advanced forms of pathogen detection in the food industry. Though in its infancy, biosensing based on clustered regularly interspaced short palindromic repeats (CRISPR) has the potential to solve many problems that cannot be addressed using conventional methods. In this review, we briefly introduce and classify the various CRISPR/Cas protein effectors that have thus far been used in biosensors. We then assess the current state of CRISPR technology in food-safety contexts; describe how each Cas effector is utilized in foodborne-pathogen detection; and discuss the limitations of the current technology, as well as how it might usefully be applied in other areas of the food industry. We conclude that, if the limitations of existing CRISPR/Cas-based detection methods are overcome, they can be deployed on a wide scale and produce a range of positive food-safety outcomes.

show abstract

Section: Classification Of Crispr/cas Systemsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recent advances in CRISPR‐based systems for the detection of foodborne pathogens

Shin

Miller

Wang

2022

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…In future development, these issues can be addressed by exploiting the most recent advances in the technologies related to the different components of the sensors and biosensors. For example, the latest results in biotechnology are opening to the possibility of designing highly selective biosensors by tuning the affinity of the biological receptors to selected VOCs thanks to gene editing techniques [ 203 , 204 ]. Additionally, progress in microfabrication can lead to a substantial decrease in production costs, to large scale fabrication of nominally identical structures, and to the possibility of integrating different sensors and biosensors [ 205 ].…”

Section: Conclusion: Challenges and Opportunitiesmentioning

confidence: 99%

Paving the Way for a Green Transition in the Design of Sensors and Biosensors for the Detection of Volatile Organic Compounds (VOCs)

et al. 2022

View full text Add to dashboard Cite

The efficient and selective detection of volatile organic compounds (VOCs) provides key information for various purposes ranging from the toxicological analysis of indoor/outdoor environments to the diagnosis of diseases or to the investigation of biological processes. In the last decade, different sensors and biosensors providing reliable, rapid, and economic responses in the detection of VOCs have been successfully conceived and applied in numerous practical cases; however, the global necessity of a sustainable development, has driven the design of devices for the detection of VOCs to greener methods. In this review, the most recent and innovative VOC sensors and biosensors with sustainable features are presented. The sensors are grouped into three of the main industrial sectors of daily life, including environmental analysis, highly important for toxicity issues, food packaging tools, especially aimed at avoiding the spoilage of meat and fish, and the diagnosis of diseases, crucial for the early detection of relevant pathological conditions such as cancer and diabetes. The research outcomes presented in the review underly the necessity of preparing sensors with higher efficiency, lower detection limits, improved selectivity, and enhanced sustainable characteristics to fully address the sustainable manufacturing of VOC sensors and biosensors.

show abstract

“…CRISPR/Cas system, which normally plays the role of a bacterial immune systems [ 14 , 15 ], has been developed into powerful tools for genome editing [ 16 , 17 ], genome imaging [ 18 ], cell imaging [ [19] , [20] , [21] ], and disease treatment [ [22] , [23] , [24] ]. The CRISPR/Cas system is also a promising platform for next-generation molecular detection technologies with high sensitivity and specificity that require no special instrumentation [ 25 , 26 ]. The trans-cleavage activity of Cas effector proteins (such as Cas12, Cas13, Cas14, etc.)…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode

Wang²,

Wu³

et al. 2022

Analytica Chimica Acta

View full text Add to dashboard Cite

Advancing sensing technology with CRISPR: From the detection of nucleic acids to a broad range of analytes – A review

Cited by 48 publications

References 128 publications

Recent advances in CRISPR‐based systems for the detection of foodborne pathogens

Recent advances in CRISPR‐based systems for the detection of foodborne pathogens

Paving the Way for a Green Transition in the Design of Sensors and Biosensors for the Detection of Volatile Organic Compounds (VOCs)

Ultrasensitive SARS-CoV-2 diagnosis by CRISPR-based screen-printed carbon electrode

Contact Info

Product

Resources

About