A CRISPR-enabled fluorometric biosensor for the sensitive detection of heparin antidote protamine based on programmable nuclease Cas12a

Ji, Zhirun; Shang, Zhaoyang; Sohail, Muhammad; Wang, Pei; Li, Bingzhi; Zhang, Xing; Chen, Guoxiang

doi:10.1016/j.snb.2022.132709

Cited by 15 publications

(5 citation statements)

References 44 publications

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“…Due to these efforts, polysaccharides-which have distinct and particular properties-have been the preferred option in several applications. [63][64][65]…”

Section: Advantages and Disadvantages Of Polysaccharides In Sensors A...mentioning

confidence: 99%

Polysaccharides Based Biosensors for Medical Applications: Prospective and Future Aspects

2023

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Biopolymers have attracted much of interest in various applications needed for sustainable and biodegradable solutions. Indeed, biopolymers lessen the requirement for petroleum‐based resources, municipal solid waste output, and carbon dioxide emissions. Polysaccharides are a unique category of biopolymers that are especially characteristic such as biocompatibility and editability. Polysaccharides functionalization plays a significant role in various applications and their suitability for additive applications. Sensor‐based polysaccharides are widely used with many drawbacks localized in durability, moldability, and sensitivity. Otherwise, biosensors are devices monitoring a specimen's biological phenomena or measurements, usually not disposable devices. For that, biosensors are needed urgently to address the high cost of disposable supplies in the medical diagnostic industry, which span many elements of medical diagnostic applications. Additionally, manipulating of polysaccharide‐based materials is considered accessible and usable and requires low equipment requirements. Herein the main features of polysaccharides will be discussed, along with potential applications as biosensors for medical diagnosis and new lines of inquiry with such focus on the main required to formulate polysaccharides biosensors.This article is protected by copyright. All rights reserved

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“…Due to these efforts, polysaccharides-which have distinct and particular properties-have been the preferred option in several applications. [63][64][65]…”

Section: Advantages and Disadvantages Of Polysaccharides In Sensors A...mentioning

confidence: 99%

Polysaccharides Based Biosensors for Medical Applications: Prospective and Future Aspects

2023

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“…Cas12a can be guided to cut almost any sequence of interest by altering the crRNA sequences. − After RNP binds to the target DNA, Cas12a sequentially cuts each strand of DNA through the RuvC nuclease domain by cis cleavage and, meanwhile, nonspecifically cuts nearby ssDNA through trans cleavage . The target DNA-induced cleavage of Cas12a provides a flexible and convenient way for both genome editing and bioanalysis. , In particular, CRISPR/Cas12-based analytical systems possess high specificity, sensitivity, and programmability, revolutionizing the field of diagnostics and biosensing. − …”

Section: Introductionmentioning

confidence: 99%

Heparin Specifically Inhibits CRISPR/Cas12 Activation, Enabling Ultrasensitive Heparin Detection and Gene Editing Regulation

Cao,

Bian,

et al. 2024

Anal. Chem.

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Heparin is a highly sulfated linear glycosaminoglycan that is used as an anticoagulant to prevent and treat thrombotic diseases. Herein, we find that heparin specifically inhibits the activation of the Cas12 protein through the competitive binding of heparin and crRNA to Cas12. Studies illustrate that heparin's high molecular weight and strong negative charge are critical parameters for its inhibitory effect. This unexpected finding was engineered for the detection of heparin, affording a low detection limit of 0.36 ng/mL for fluorometric quantification. We further developed a rapid lateral flowbased system named HepaStrip (heparin strip), allowing heparin monitoring in clinical samples within 20 min. Finally, in vivo investigations revealed that heparin can regulate gene editing with the clusters of the regularly spaced short palindromic repeat (CRISPR)/Cas12 system in Escherichia coli. Heparin blocks the formation of Cas12-crRNA ribonucleoprotein, allowing the application of CRISPR for rapid and field-deployable detection of heparin and unleashing the potential use of heparin in future anti-CRISPR applications.

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“…25 African swine fever virus (ASFV), 26 human immunodeciency virus (HIV), 27 displaying obvious advantages of mild reaction conditions, operational simplicity, great sensitivity, and potent signal amplication. 28,29 Although some CRISPR-based MPXV assays have been reported, 30,31 the lack of systematic design and screening of primers and crRNA has resulted in insufficient sensitivity, leaving the potential of CRISPR techniques in MPXV prevention insufficiently explored.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas12a-mediated ultrasensitive and on-site monkeypox viral testing

Zhao

Wang

et al. 2023

Anal. Methods

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A CRISPR-enabled fluorometric biosensor for the sensitive detection of heparin antidote protamine based on programmable nuclease Cas12a

Cited by 15 publications

References 44 publications

Polysaccharides Based Biosensors for Medical Applications: Prospective and Future Aspects

Polysaccharides Based Biosensors for Medical Applications: Prospective and Future Aspects

Heparin Specifically Inhibits CRISPR/Cas12 Activation, Enabling Ultrasensitive Heparin Detection and Gene Editing Regulation

CRISPR/Cas12a-mediated ultrasensitive and on-site monkeypox viral testing

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