Evaluation of the antioxidant activity of Coreopsis tinctoria Nuff. and optimisation of isolation by response surface methodologyThe aim of the present study was to determine the antioxidant activity of Coreopsis tinctoria flowering tops (CTFT). Studies were conducted to obtain suitable extraction conditions for chlorogenic acid, quercetin, luteolin, apigenin and kaempferol, which were identified and quantified by HPLC. Response surface methodology was employed to optimise the ultrasound-assisted extraction conditions including extraction time, ethanol concentration and liquid-solid ratio. The antioxidant activity of the extracts was analysed using various antioxidant models, such as DPPH, ABTS and hydroxyl radical scavenging assay. CTFT extracted for 15.0 min with ethanol at a concentration of 60.4 % and with liquidsolid ratio 27.5:1 possessed a considerable amounts of total flavonoids and polyphenols (18.9 %). This extract showed higher scavenging activity of ABTS and hydroxyl radical activity than rutin, however not in the DPPH test. We may assume that CTFT possess antioxidant and free radical scavenging potentials.
The purpose of this work was to assess the antifungal activity of total flavonoids extract from Patrinia Villosa Juss. Response surface methodology was employed to optimize the main extraction conditions including extraction time, ethanol concentration and solid-liquid ratio. The effect of total flavonoids extract from P. villosa on Microsporum gypseum, Trichophyton mentagrophytes and Trichophyton rubrun were studied. We found that under the conditions of extraction time 25.51 min, solvent concentration 62.82% and solid-liquid ratio to be 27.39:1, it possesses considerable amounts of flavonoids of 8.1987 mg/g rutin equivalent of extract. The effects of antifungal activity of P. villosa on Trichophyton were more sensitive than Microsporum, but less effective than the positive control ketoconazole. In conclusion, total flavonoids extract from P. villosa possess potent anti-dermatophyte activities. These activities could contribute, at least in part, to the traditionally claimed therapeutic benefits of P. villosa.
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