Evaluation to the antifungal activity of total flavonoids extract from Patrinia Villosa Juss and optimization by response surface methodology

Ni, Zheng

doi:10.5897/ajmr11.1393

Cited by 14 publications

(3 citation statements)

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“…Here, we identify tripartite ClyA family toxins in the genomes of medically and economically important pathogenic Gram-negative bacteria, including Serratia marcescens , a causative agent of nosocomial infections 25 ; Erwinia mallotivora , responsible for papaya dieback disease 26 , resulting in $58 million/annum damage to crops in Malaysia; and highly virulent strains of Aeromonas hydrophila , which cause fatal haemorrhagic septicaemia in a number of important farmed fish species with epidemics in US and Asian aquaculture causing multimillion dollar losses 27,28 , as well as being a contributor to opportunistic human disease 29 . We show that the orthologue identified in A. hydrophila (AhlABC) is a haemolytic tripartite α-PFT, requiring all three components for maximal lysis, and we present the structures of the soluble forms of monomeric AhlB and tetrameric AhlC and the decameric pore form of AhlB.…”

Section: Introductionmentioning

confidence: 99%

Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila

et al. 2019

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The alpha helical CytolysinA family of pore forming toxins (α-PFT) contains single, two, and three component members. Structures of the single component Eschericia coli ClyA and the two component Yersinia enterolytica YaxAB show both undergo conformational changes from soluble to pore forms, and oligomerization to produce the active pore. Here we identify tripartite α-PFTs in pathogenic Gram negative bacteria, including Aeromonas hydrophila (AhlABC). We show that the AhlABC toxin requires all three components for maximal cell lysis. We present structures of pore components which describe a bi-fold hinge mechanism for soluble to pore transition in AhlB and a contrasting tetrameric assembly employed by soluble AhlC to hide their hydrophobic membrane associated residues. We propose a model of pore assembly where the AhlC tetramer dissociates, binds a single membrane leaflet, recruits AhlB promoting soluble to pore transition, prior to AhlA binding to form the active hydrophilic lined pore.

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Section: Introductionmentioning

confidence: 99%

Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila

et al. 2019

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“…Optimal conditions for the extraction of phenolic compounds from M. vulgare leaves were obtained using response surface methodology (RSM) [41] [42]. This method is adopted for each type of phenolic compound with the solvent giving the highest experimental concentration.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Extraction Conditions of Some Phenolic Compounds from White Horehound (<i>Marrubium vulgare</i> L.) Leaves

Bouterfas¹,

Mehdadi²,

Benmansour³

et al. 2014

IJOC

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This research was aimed to optimize the extraction conditions of three phenolic compounds: total phenolics, flavonoids and condensed tannins, from White Horehound's leaves (Marrubium vulgare L.). Distilled water and different organic solvents such as: methanol, ethanol and acetone, were used, with various concentrations (20% -80%, v/v), temperatures (20˚C -60˚C) and extraction times (30 -450 min). Results showed that the maximum total phenolics amounts (293.34 ± 14.60 mg gallic acid equivalent/g dry weigh), were obtained with 60% aqueous methanol at 25˚C for 180 min; total flavonoids (79.52 ± 0.55 mg catechin equivalent/g dry weigh) with 80% aqueous methanol at 20˚C for 450 min, and condensed tannins (28.15 ± 0.80 mg catechin equivalent/g dry weigh) with 60% aqueous acetone at 50˚C and for 180 min. ANOVA test showed the significant effect ( *** P < 0.001) of the extraction conditions tested on phenolic compounds. The Principal Component Analysis (PCA) exhibited the positive effect of low temperatures on total phenolics and flavonoids extraction, and the effect of high temperatures on the condensed tannins extraction. The Response Surface Methodology (RSM) provided predicted values of extraction conditions and maximum polyphenols amounts similar to those obtained experimentally. IntroductionPolyphenols constitute one of the most common and widespread groups of substances in flowering plants, occurring in all vegetative organs, as well as in flowers and fruits. These molecules are involved in many physiological processes such as cell growth, root formation, seed germination and fruit ripening [1]. Moreover, these compounds are considered secondary metabolites involved in the chemical defense of plants against predators, pathogens, environmental stresses and in plant-plant interferences [2].Nowadays, phenolic compounds represent a unique and a functional place, composed of bioactive products, present in plant-derived foods and beverages and included in the formulations of well-marketed cosmetic and parapharmaceutical products [3]. Furthermore, polyphenols exhibit various biological activities such as anticancer [4], antioxidant [5], antimicrobial [6] and anti-inflammatory activities [7]. Therefore, in recent years, the determination of phenolic compounds concentrations in fruits [8] [9], vegetables [10] and some aromatic and medicinal plants [11] [12] has been of increasing interest in the scientific community as well as among health professionals and business partners. It is well known that the content of phenolic compounds could be influenced by environmental conditions, such as season [13] [14], sampling period and geographic origin [15], precipitations and temperatures [16], and soil type [17]. Additionally, there are several experimental factors that can influence the rate of extraction and the quality of extracted bioactive phenolic compounds. These factors include extraction method, solvent type used and concentration [18], particle size of medicinal plants, temperature and pH of extraction [19], extraction t...

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“…The content of flavonoids was determined by using a colorimetric method described by Zheng et al (2012) with some modifications. Briefly, the extraction solution and different concentrations of the rutin standards solution were diluted appropriately and mixed with …”

Section: Determination Of Flavonoid Contentmentioning

confidence: 99%

In vitroantioxidant and antimicrobial activities of flavonoids fromPanax notoginsengflowers

Hong

Liu

et al. 2014

Natural Product Research

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Panax notoginseng and its flowers are both well-known traditional Chinese medicinal herbs. To date, antimicrobial and antioxidant activities of flavonoids from P. notoginseng flowers (PNF) remain unclear. In this study, antimicrobial and antioxidant activities of flavonoids from PNF were investigated. The crude flavonoids were purified using a column (25 cm × 1.5 cm) packed with AB-8 macroporous adsorption resin. Compared with ascorbic acid, the purified flavonoids excelled in scavenging activities on 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid, superoxide anion and hydroxyl radicals at 0.2-1.0 mg/mL concentration. However, flavonoids exhibited weaker reducing power than ascorbic acid at 20-100 μg/mL concentration. In addition, the flavonoids exhibited obvious inhibitory effects on Staphylococcus aureus, Aeromonas hydrophila and Pseudomonas aeruginosa. These results clearly indicate that flavonoids from PNF are effective in scavenging free radicals and have the potential to be used as antioxidants and antimicrobial agents, and also provide the theoretical data for supporting the use of PNF in food, pharmaceutical and cosmetics industries.

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Evaluation to the antifungal activity of total flavonoids extract from Patrinia Villosa Juss and optimization by response surface methodology

Cited by 14 publications

References 14 publications

Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila

Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila

Optimization of Extraction Conditions of Some Phenolic Compounds from White Horehound (<i>Marrubium vulgare</i> L.) Leaves

In vitroantioxidant and antimicrobial activities of flavonoids fromPanax notoginsengflowers

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Evaluation to the antifungal activity of total flavonoids extract from Patrinia Villosa Juss and optimization by response surface methodology

Cited by 14 publications

References 14 publications

Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila

Identification and structural analysis of the tripartite α-pore forming toxin of Aeromonas hydrophila

Optimization of Extraction Conditions of Some Phenolic Compounds from White Horehound (&lt;i&gt;Marrubium vulgare&lt;/i&gt; L.) Leaves

In vitroantioxidant and antimicrobial activities of flavonoids fromPanax notoginsengflowers

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Optimization of Extraction Conditions of Some Phenolic Compounds from White Horehound (<i>Marrubium vulgare</i> L.) Leaves