We
describe a hand-held sensing system using a transistor based
multiplexed platform and a detector that couples the electrochemical
information wirelessly to a smartphone. The custom disposable platform
exploits the ion-sensitive FET (ISFET) technology. Via simple surface
modifications the design allows a broad range of analytes to be tested
with low cost. We compared our read-out device to a commercial potentiometer
using K+ as an example species analyte. The developed sensing
system has a slightly better limit of detection and is notably less
susceptible to external noise which is commonly observed with potentiometers.
The designed platform is fabricated using standard electronic processes
with gold surface and we used commercial discrete transistors as the
transducing element. It can be mass produced with high yield and low
cost. To circumvent the drift that typically occurs with modified
solid state electrodes we incorporated a transducing layer between
the electric conductor (gold pad) and the ionically conducting ion-selective
membrane. The polyaniline doped with dinonylnaphtalene sulfonic acid
(PANI-DNNSA) was used as a transducing layer for the first time. The
PANI-DNNSA layer significantly reduces the drift of the electrodes
compared to a configuration without the transducing layer. The system
is easy to use with a transistor based detection that can be modified
for a vast variety of existing potentiometric tests.
Out of 463 million people currently with diabetes, 232 million remain undiagnosed. Diabetes is a threat to human health, which could be mitigated via continuous self-monitoring of glucose. In addition to blood, interstitial fluid is considered to be a representative sample for glucose monitoring, which makes it highly attractive for wearable on-body sensing. However, new technologies are needed for efficient and noninvasive sampling of interstitial fluid through the skin. In this report, we introduce the use of Lorentz force and magnetohydrodynamics to noninvasively extract dermal interstitial fluid. Using porcine skin as an ex-vivo model, we demonstrate that the extraction rate of magnetohydrodynamics is superior to that of reverse iontophoresis. This work seeks to provide a safe, effective, and noninvasive sampling method to unlock the potential of wearable sensors in needle-free continuous glucose monitoring devices that can benefit people living with diabetes.
We demonstrate an electrochemical sensor for detection of unlabeled single-stranded DNA using peptide nucleic acid (PNA) probes coupled to the field-effect transistor (FET) gate. The label-free detection relies on the intrinsic charge of the DNA backbone. Similar detection schemes have mainly concentrated on sensitivity improvement with an emphasis on new sensor structures. Our approach focuses on using an extended-gate that separates the FET and the sensing electrode yielding a simple and mass fabricable device. We used PNA probes for efficient hybridization in low salt conditions that is required to avoid the counter ion screening. As a result, significant part of the target DNA lies within the screening length of the sensor. With this, we achieved a wash-free detection where typical gate potential shifts are more than 70 mV with 1 µM target DNA. We routinely obtained a real-time, label- and wash-free specific detection of target DNA in nanomolar concentration with low-cost electronics and the responses were achieved within minutes after introducing targets to the solution. Furthermore, the results suggest that the sensor performance is limited by specificity rather than by sensitivity and using low-cost electronics does not limit the sensor performance in the presented sensor configuration.
Electrically
conductive composite nanofibers were fabricated using
poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate)
(PEDOT–PSS) and cellulose nanofibrils (CNFs) via the electrospinning
technique. Poly(ethylene oxide) (PEO) was used to assist the electrospinning
process, and poly(ethylene glycol) diglycidyl ether was used to induce
chemical cross-linking, enabling stability of the formed fibrous mats
in water. The experimental parameters regarding the electrospinning
polymer dispersion and electrospinning process were carefully studied
to achieve a reproducible method to obtain bead-free nanofibrous mats
with high stability after water contact, with an electrical conductivity
of 13 ± 5 S m–1, thus making them suitable
for bioelectrochemical applications. The morphology of the electrospun
nanofibers was characterized by scanning electron microscopy, and
the C/S ratio was determined with energy dispersive X-ray analysis.
Cyclic voltammetric studies showed that the PEDOT–PSS/CNF/PEO
composite fibers exhibited high electroactivity and high stability
in water for at least two months. By infrared spectroscopy, the slightly
modified fiber morphology after water contact was demonstrated to
be due to dissolution of some part of the PEO in the fiber structure.
The biocompatibility of the PEDOT–PSS/CNF/PEO composite fibers
when used as an electroconductive substrate to immobilize microalgae
and cyanobacteria in a photosynthetic bioelectrochemical cell was
also demonstrated.
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