The objective of the study was to investigate the distribution of LPIN2 variants and haplotypes among breeds and perform an association analysis of the variants and haplotypes with the broiler traits in chickens. Six breeds were used to study the variation and distribution of chicken LPIN2, and an F2 resource population was used to measure growth traits, carcass traits, meat quality traits and serum biochemistry parameters. A c.-599G>A variant was located in the promoter region of LPIN2 and c.444G>A and c.1730A>T (E577D) coding variant mutations were detected. Linkage disequilibrium tests showed that these three variants were under moderate linkage disequilibrium in the 6 breeds and 7 haplotypes were constructed. The distribution of variation/haplotypes presented clear differences among breeds. Association analysis showed that c.-599G>A was associated with leg muscle weight, jejunum length, ileum length, leg muscle fibre density and leg muscle fibre diameter; c.444G>A was associated with spleen weight, ileum length, body weight at hatch and metatarsus length at 8 weeks; c.1730T>A had significant effects on chicken liver weight, heart weight, body weight at 10 weeks, serum albumin and glucose. Diplotypes were significantly associated with body weight at hatch, heart weight, pancreas weight, duodenum length, leg muscle fibre density and lactate dehydrogenase.
In the transforming growth factor-β(TGF-β) superfamily, bone morphogenetic proteins (BMPs) are one of the largest subfamily, among which BMP-2 and BMP-4 are the most powerful molecules in biological activities. They have been shown to be involved in follicle development of ovary in mammals. However, whether these factors are involved in folliculogenesis in rabbit is still unknown. This study was performed to determine the relationships between ovarian development and the expression of BMP (BMP-2,-4) mRNAs in rabbit. Ovaries were removed from different development stages of rabbits (30-, 60-, 120-, 180-day age and 6-day encyesis), and method of RT-PCR was used for the cloning. The relative transcript levels of BMP-2,-4 genes were measured by real-time quantitative PCR, with GAPDH as an internal control. Known from the results, the BMP-2,-4 genes showed similar but also different expression patterns during ovarian development; they both increased significantly (p < 0.05) in the ovary on 60-day compared with 120-day and 6-day encyesis, and the expression of BMP-2 was always higher than that of BMP-4. The expression law of BMP-2,-4 genes presented in this study may lay the theoretical foundation for the study of BMPs' biological function.
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