In this paper, we proposed a substrate rotation‐enhanced Raman scattering (SRERS) effect. The substrate, prepared by the technology of texturization and electroplating, is fixed on the motor and rotates with it while collecting Raman signal. The relative standard deviation (RSD) of Raman intensity in multiple measurements is less than 9.1%, which indicates that the Raman signal is stable and reproducible based on the SRERS effect. What is more, this method eliminates the fluctuation of the local electric field intensity caused by the inhomogeneity of the substrate and makes the “equivalent hot spots” distributed uniformly on the whole surface. The quantitative analysis with the Rhodamine 6G (R6G) and the lipid‐soluble drug Oxaprozin (OXA) as the probe respectively has demonstrated that the Raman intensity of R6G at 612 cm−1 with respect to concentration tend to linear ranging from 10−10 to 10−8 mol/L with the correlation coefficient (R2) is more than 0.96, and the Raman intensity of OXA at 1,003 cm−1 with respect to concentration tend to linear ranging from 3.4 * 10−6 to 3.4 * 10−4 mol/L (prepared with 50% v/v ethanol) with the correlation coefficient (R2) is more than 0.97. The mechanism of SRERS is given, and the theory analysis indicates that the Raman signal obtained is the mean of a large number of laser‐scanning points' contributions.
In sports events, the rapid recovery after high-intensity training or sport competition performance is very important for athletes’ performance and health. The aim of this study is to evaluate the effect of laser acupuncture and electrical stimulation on the recovery from exercise fatigue, using mice with swimming fatigue as experimental model and the electromyography (EMG) and the Raman spectroscopy of blood as evaluation indicators. Root mean square (RMS) and mean power frequency (MPF) of EMG were analyzed after laser acupuncture and electrical stimulation. The amplitude frequency combined analysis (JASA) showed that the proportion of muscles in the fatigue recovery area of the control group, the laser acupuncture group, the multi-channel laser acupuncture group and the laser combined with electrical stimulation group were 34.78%, 39.13%, 39.13% and 43.48%, respectively. Raman spectroscopy of the mice blood during fatigue recovery showed there is a significant difference between the multi-channel laser acupuncture group and the laser combined with electric stimulation group compared with the recovery period and fatigue period ([Formula: see text]) at the peak of 997[Formula: see text]cm[Formula: see text] and the laser combined electrical stimulation group had a statistical difference in the recovery period compared with the fatigue period ([Formula: see text]) at the peak of 1561[Formula: see text]cm[Formula: see text]. The results showed that laser acupuncture combined with electrical stimulation was beneficial to fatigue recovery in mice, and had the potential value in sports fatigue recovery.
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