Rapid and high‐precision quantitative analysis based on substrate rotation‐enhanced Raman scattering effect

Abstract

In this paper, we proposed a substrate rotation‐enhanced Raman scattering (SRERS) effect. The substrate, prepared by the technology of texturization and electroplating, is fixed on the motor and rotates with it while collecting Raman signal. The relative standard deviation (RSD) of Raman intensity in multiple measurements is less than 9.1%, which indicates that the Raman signal is stable and reproducible based on the SRERS effect. What is more, this method eliminates the fluctuation of the local electric field intensity caused by the inhomogeneity of the substrate and makes the “equivalent hot spots” distributed uniformly on the whole surface. The quantitative analysis with the Rhodamine 6G (R6G) and the lipid‐soluble drug Oxaprozin (OXA) as the probe respectively has demonstrated that the Raman intensity of R6G at 612 cm−1 with respect to concentration tend to linear ranging from 10−10 to 10−8 mol/L with the correlation coefficient (R2) is more than 0.96, and the Raman intensity of OXA at 1,003 cm−1 with respect to concentration tend to linear ranging from 3.4 * 10−6 to 3.4 * 10−4 mol/L (prepared with 50% v/v ethanol) with the correlation coefficient (R2) is more than 0.97. The mechanism of SRERS is given, and the theory analysis indicates that the Raman signal obtained is the mean of a large number of laser‐scanning points' contributions.

Ag Nanoparticles for the Direct Detection of Oxaprozin in the Blood Using Surface-Enhanced Raman Spectroscopy

“…The substrate rotation Raman technique means that the SERS substrate is fixed on the motor and rotates with it while collecting Raman signal. The RSD of the Raman intensity in multiple measurements is less than 6.5% when using the substrate rotation technique to detect OXA solution, 41 which indicates that the Raman signal is stable and reproducible.…”

Unveiling practical considerations for reliable and standardized SERS measurements: lessons from a comprehensive review of oblique angle deposition-fabricated silver nanorod array substrates

“…To address these issues, a rastering/translating method can be employed, where the laser spot rapidly moves across the substrate surface during excitation/measurement without altering the optical signal collection configuration. [182][183][184][185][186] This method increases the measuring area by dynamically rastering or translating the laser spot during the measurement, while keeping the SERS instrument configuration intact. By reducing the residence time of the laser at a particular location and simultaneously generating a signal that is essentially a summed average of multiple locations on the substrate, this approach significantly improves quantitative SERS analysis.…”