Gastric cancer was classified as the third most deadly cancer among all other cancer types. The HSP90 and HER2 genes play essential roles in the stability and function of high-expression proteins that cause malignancy. The aim of this research was to investigate the influence of the alcoholic
Alkanna bracteosa
extract on the expression of HSP90α and HER2 genes in AGS cell line. Therefore, the methanolic extraction was isolated from aerial parts of the plant and AGS and HuGu cell lines were analyzed using 102.4–0.05 mg ml
−1
dose concentrations in serial dilution; to measure the cell toxicity by MTT assay. Furthermore, real-time PCR analysis measured the expression level of HSP90α and HER2 genes using the IC50 dose concentrations. Quantification of apoptosis was analyzed by Annexin/PI kit in flow cytometry and DNA fragmentation tests. The results of MTT assay represented the IC50 dose concentration of 0.8 and 3.2 mg ml
−1
for AGS and HuGu respectively. The rate of HER2 gene expression was significantly decreased in AGS cells treated with 0.8 mg ml
−1
dose concentration compared to control. The exposure of AGS treated cells with 0.8 mg ml
−1
dose concentration after 24 h represented 24.3% apoptosis and 13.3% necrosis. The agarose gel represented the DNA fragmentation pattern of apoptosis. This study demonstrated the significant differences between the cell viability rate, gene expression level, and apoptosis of the
Alkanna bracteosa
extract on AGS cells. These results demonstrated the first report of which the
Alkanna braceteosa
would be an effective candidate for possible treatment of Gastric cancer.
The moderate static magnetic fields (SMFs) have been used here as a non-invasive tool to study their manipulative effects on the olfactory ensheathing cells (OECs) activity, growth, morphology, and migration in culture. The OECs are involved in the regeneration of primary olfactory sensory neurons and migration into the central nervous system to repair axons damaged by infection, injury, etc., that play a pivotal role in complementary regenerative medicine. Here, OECs were isolated from the olfactory bulb and cultured to confluence. An in vitro wound healing model was formed and exposed to either parallel (PaSMF) or perpendicular (PeSMF) SMF at intensities of 30, 50, and 70 mT, and cells' morphology, podia formation, proliferation, and migration were studied by time-lapse recording. The SMFs were not cytotoxic at the intensity and exposure time applied here. The exposure of cells to 70 mT PaSMF and PeSMF increased the formation of lamellipodia and filopodia, cell migration speed, and direction of the scratch forefront cells, significantly. Treatment of cells with 70 mT PaSMF and PeSMF increased cell divisions, while 30 mT PaSMFdecreased it. SMF effects on OECs division, motility, migratory direction, and velocity indicate its effect on various aspects of cell physiology and signaling at atomic and molecular levels, and have a role in tissue regeneration that involves microtubules and actin filaments formation and rearrangements. Thus, the exposure of OECs with moderate SMF might be considered a promising noninvasive approach to remotely manipulate normal and stem cell activities for therapeutic regenerative purposes in various tissues including the central nervous system.
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