The lecithin:cholesterol acyltransferase (LCAT) activity and lysolecithin content of human blood stored under standard blood transfusion service conditions at 4 degrees C for 6 weeks has been investigated. Cooling the blood to 4 degrees C rapidly inactivates the LCAT reaction, but the enzyme is not denatured during storage under these conditions. Citrate in the anticoagulant did not activate the LCAT reaction in freshly-taken whole blood. The total phospholipid and total lysolecithin content of whole blood decreased during storage at 4 degrees C for 6 weeks. The lysolecithin content of fresh red cells (2.0-3.0 mumol lysolecithin x 10(-11) per cell) showed no significant change during the storage period.
The lecithin:cholesterol acyltransferase (LCAT) activity and lysolecithin
content of human blood stored under standard blood transfusion service
conditions at 4 °C for 6 weeks has been investigated. Cooling the blood to 4 °C
rapidly inactivates the LCAT reaction, but the enzyme is not denatured during
storage under these conditions. Citrate in the anticoagulant did not activate the
LCAT reaction in freshly-taken whole blood. The total phospholipid and total
lysolecithin content of whole blood decreased during storage at 4 °C for 6 weeks.
The lysolecithin content of fresh red cells (2.0-3.0 µmol lysolecithin x 10^-11 per
cell) showed no significant change during the storage period.
The effect of storage in ACD at 4 degrees C on red cell agglutinability and the inhibitory properties of the plasma to blood group reagents has been studied. It was found that the variability demonstrated was related to the origin of the reagent used, particularly between human and on-human sources. The significance of these findings with regard to biochemical and morphological changes in blood on storage is discussed.
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