Changes in the Agglutinability of Red Cells and the Inhibition of Specific Agglutination by Plasma from Human Blood Taken into ACD and Stored at 4 4DgC

Longster, G. H.; Tovey, L. A. D.; Barnes, Yvonne W.; Rumsby, Martin G.

doi:10.1111/j.1423-0410.1978.tb02467.x

Vox Sanguinis

1978

DOI: 10.1111/j.1423-0410.1978.tb02467.x

|View full text |Cite

Changes in the Agglutinability of Red Cells and the Inhibition of Specific Agglutination by Plasma from Human Blood Taken into ACD and Stored at 4 4DgC

G. H. Longster

L. A. D. Tovey

Yvonne W. Barnes

et al.

Abstract: The effect of storage in ACD at 4 degrees C on red cell agglutinability and the inhibitory properties of the plasma to blood group reagents has been studied. It was found that the variability demonstrated was related to the origin of the reagent used, particularly between human and on-human sources. The significance of these findings with regard to biochemical and morphological changes in blood on storage is discussed.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1979

2000

Publication Types

Select...

Article5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 5 publications

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Topo-optical investigations of the human erythrocyte glycocalyx-age related changes

Gliesing

Stibenz

et al. 1984

Histochemistry

View full text Add to dashboard Cite

The conformational state of the glycocalyx of the intact and altered erythrocyte membrane was studied by means of the topo-optical toluidine blue reaction, i.e. induced membrane birefringence. High membrane anisotropy represents the normal glycocalyx structure and its decline represents their perturbation. The results show that the glycocalyx structure is changed during ageing of the erythrocytes in vivo as well as in vitro. During fluid preservation, in vitro ageing and vesiculation of cells in vitro, a subpopulation of cells showed a decline of membrane anisotropy, but other cells demonstrated abnormally high values. In the latter cases, there is usually a correlation to spherocytes. From this point of view, it is to be assumed that spherogenesis during cell ageing is induced by cell vesiculation. This leads to a remodelling of an intact plasmalemma. In contrast, the cell fractions which are probably non-vesiculating seem to be more or less damaged by membrane and/or plasmic hydrolases. This can be mimicked by neuraminidase and protease treatment of erythrocytes in vitro. Membrane lesions caused by freeze preservation of red blood cells are rare. The topo-optical results are interpreted according to the assumptions of the theory of membrane anisotropy, i.e. the formation of dye-stuff micelles at distinct, clustered, sialylated carbohydrate chains of the glycophorin A.

show abstract

Topo-optical investigations of the human erythrocyte glycocalyx-age related changes

Gliesing

Stibenz

et al. 1984

Histochemistry

View full text Add to dashboard Cite

show abstract

Untitled

Rusnak

Coghlan

Zelinski

et al. 2000

Molecular and Cellular Biochemistry

View full text Add to dashboard Cite

The incorporation of [1-14C]palmitic or [1-14C]oleic acid into phosphatidylcholine and the effect on blood group antigen expression were examined in human erythrocytes stored at 4 degrees C for 0-3 weeks. Blood drawn into EDTA was obtained by venepuncture from healthy volunteers. A 50% suspension of washed erythrocytes was incubated in buffer containing [1-14C]fatty acid for up to 60 min at 37 degrees C with moderate shaking. Phosphatidylcholine was extracted and analyzed for uptake of radiolabelled fatty acid and phospholipid phosphorus content. Incorporation of [1-14C]palmitic or [1-14C]oleic acid into phosphatidylcholine was reduced during storage. The mechanism for the reduction in radiolabelled fatty acid incorporation into phosphatidylcholine was a 64% (p < 0.05) reduction in membrane phospholipase A2 activity. Although human erythrocyte membranes isolated from freshly drawn blood are capable of reacylating lysophosphatidylcholine to phosphatidylcholine, with storage, a markedly different substrate preference between palmitoyl-Coenzyme A and oleoyl-Coenzyme A was observed. Lysophosphatidylcholine acyltransferase activity assayed with oleoyl-Coenzyme A was unaltered with storage. In contrast, lysophosphatidylcholine acyltransferase activity assayed with palmitoyl-Coenzyme A was elevated 5.5-fold (p < 0.05). Despite these changes, storage of erythrocytes for up to 3 weeks did not result in altered expression of the various blood group antigens investigated. We conclude that the incorporation of palmitate and oleate into phosphatidylcholine is dramatically reduced during storage of human erythrocytes. The observed differential in vitro substrate utilization suggests that distinct acyltransferases are involved in the acylation of lysophosphatidylcholine to phosphatidylcholine in human erythrocytes.

show abstract

Duffy Antigen Changes on Red Blood Cells Stored at Low Temperature

1981

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Changes in the Agglutinability of Red Cells and the Inhibition of Specific Agglutination by Plasma from Human Blood Taken into ACD and Stored at 4 4DgC

Cited by 5 publications

References 13 publications

Topo-optical investigations of the human erythrocyte glycocalyx-age related changes

Topo-optical investigations of the human erythrocyte glycocalyx-age related changes

Untitled

Duffy Antigen Changes on Red Blood Cells Stored at Low Temperature

Contact Info

Product

Resources

About